Isolation and characterization of microsatellite markers in the liver fluke ( Fasciola hepatica )

Six microsatellite markers were isolated from Fasciola hepatica , a re-emerging parasite that causes important veterinary and public health problems. In a sample of 52 liver flukes from a region of hyperendemicity (Bolivian Altiplano), five microsatellite were polymorphic. Our results showed that liver flukes present important genetic variability, suggesting a preferential outcrossing reproduction mode for this hermaphroditic parasite.