Retention of transcription initiation factor sigma70 in transcription elongation: single-molecule analysis.

[1]  Nam Ki Lee,et al.  Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation. , 2005, Biophysical journal.

[2]  R. Ebright,et al.  The interaction between σ70 and the β-flap of Escherichia coli RNA polymerase inhibits extension of nascent RNA during early elongation , 2005 .

[3]  R. Ebright,et al.  The interaction between sigma70 and the beta-flap of Escherichia coli RNA polymerase inhibits extension of nascent RNA during early elongation. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[4]  K. Struhl,et al.  Association of RNA polymerase with transcribed regions in Escherichia coli. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[5]  Nam Ki Lee,et al.  Fluorescence-aided molecule sorting: Analysis of structure and interactions by alternating-laser excitation of single molecules , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[6]  Jayanta Mukhopadhyay,et al.  The σ70 subunit of RNA polymerase mediates a promoter-proximal pause at the lac promoter , 2004, Nature Structural &Molecular Biology.

[7]  R. Ebright,et al.  Promoter unwinding and promoter clearance by RNA polymerase: detection by single-molecule DNA nanomanipulation. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[8]  D. Swigon,et al.  Catabolite activator protein: DNA binding and transcription activation. , 2004, Current opinion in structural biology.

[9]  N. Zenkin,et al.  The sigma 70 subunit of RNA polymerase induces lacUV5 promoter-proximal pausing of transcription. , 2004, Nature structural & molecular biology.

[10]  Jayanta Mukhopadhyay,et al.  The sigma 70 subunit of RNA polymerase mediates a promoter-proximal pause at the lac promoter. , 2004, Nature structural & molecular biology.

[11]  R. Landick,et al.  Tethering σ70 to RNA polymerase reveals high in vivo activity of σ factors and σ70-dependent pausing at promoter-distal locations , 2003 .

[12]  R. Ebright,et al.  Fluorescence resonance energy transfer (FRET) in analysis of transcription-complex structure and function. , 2003, Methods in enzymology.

[13]  R. Landick,et al.  Tethering sigma70 to RNA polymerase reveals high in vivo activity of sigma factors and sigma70-dependent pausing at promoter-distal locations. , 2003, Genes & development.

[14]  K. Severinov,et al.  Role of the RNA polymerase sigma subunit in transcription initiation. , 2002, Research in microbiology.

[15]  Jeffrey W. Roberts,et al.  The σ70 Subunit of RNA Polymerase Is Contacted by the λQ Antiterminator during Early Elongation , 2002 .

[16]  S. Yokoyama,et al.  Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 Å resolution , 2002, Nature.

[17]  K. Murakami,et al.  Structural Basis of Transcription Initiation: RNA Polymerase Holoenzyme at 4 Å Resolution , 2002, Science.

[18]  K. Murakami,et al.  Structural Basis of Transcription Initiation: An RNA Polymerase Holoenzyme-DNA Complex , 2002, Science.

[19]  Jennifer L. Knight,et al.  Structural Organization of Bacterial RNA Polymerase Holoenzyme and the RNA Polymerase-Promoter Open Complex , 2002, Cell.

[20]  Jeffrey W. Roberts,et al.  The sigma(70) subunit of RNA polymerase is contacted by the (lambda)Q antiterminator during early elongation. , 2002, Molecular cell.

[21]  R. Ebright,et al.  Mean DNA bend angle and distribution of DNA bend angles in the CAP-DNA complex in solution. , 2001, Journal of molecular biology.

[22]  E. Nudler,et al.  Isolation and Characterization of σ70-Retaining Transcription Elongation Complexes from Escherichia coli , 2001, Cell.

[23]  R. Ebright,et al.  Translocation of σ70 with RNA Polymerase during Transcription Fluorescence Resonance Energy Transfer Assay for Movement Relative to DNA , 2001, Cell.

[24]  R. Ebright RNA polymerase: structural similarities between bacterial RNA polymerase and eukaryotic RNA polymerase II. , 2000, Journal of molecular biology.

[25]  S. Darst,et al.  A Structural Model of Transcription Elongation , 2000 .

[26]  Michelle D. Wang,et al.  Force and velocity measured for single molecules of RNA polymerase. , 1998, Science.

[27]  C. Gross,et al.  The functional and regulatory roles of sigma factors in transcription. , 1998, Cold Spring Harbor symposia on quantitative biology.

[28]  C. Turnbough,et al.  Transcription regulation by initiating NTP concentration: rRNA synthesis in bacteria. , 1997, Science.

[29]  E. Nudler,et al.  The RNA–DNA Hybrid Maintains the Register of Transcription by Preventing Backtracking of RNA Polymerase , 1997, Cell.

[30]  M. Kashlev,et al.  Transcriptional arrest: Escherichia coli RNA polymerase translocates backward, leaving the 3' end of the RNA intact and extruded. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[31]  Jeffrey W. Roberts,et al.  Function of E. coli RNA Polymerase σ Factor- σ70 in Promoter-Proximal Pausing , 1996, Cell.

[32]  J. Roberts,et al.  Function of E. coli RNA polymerase sigma factor sigma 70 in promoter-proximal pausing. , 1996, Cell.

[33]  P. V. von Hippel,et al.  RNA displacement pathways during transcription from synthetic RNA-DNA bubble duplexes. , 1994, Biochemistry.

[34]  H. Heumann,et al.  Nucleation of RNA chain formation by Escherichia coli DNA-dependent RNA polymerase. , 1993, Journal of molecular biology.

[35]  O. Miller,et al.  rRNA transcription rate in Escherichia coli , 1991, Journal of bacteriology.

[36]  M. Sheetz,et al.  Transcription by single molecules of RNA polymerase observed by light microscopy , 1991, Nature.

[37]  P. V. von Hippel,et al.  Escherichia coli sigma 70 and NusA proteins. I. Binding interactions with core RNA polymerase in solution and within the transcription complex. , 1991, Journal of molecular biology.

[38]  M. Chamberlin,et al.  RNA chain initiation by Escherichia coli RNA polymerase. Structural transitions of the enzyme in early ternary complexes. , 1989, Biochemistry.

[39]  A. Woody,et al.  Transcription initiation by Escherichia coli RNA polymerase at the gene II promoter of M13 phage: stability of ternary complex, direct photocrosslinking to nascent RNA, and retention of sigma subunit. , 1987, Biochimica et biophysica acta.

[40]  D. Crothers,et al.  A stressed intermediate in the formation of stably initiated RNA chains at the Escherichia coli lac UV5 promoter. , 1987, Journal of molecular biology.

[41]  N. Shimamoto,et al.  Release of the sigma subunit of Escherichia coli DNA-dependent RNA polymerase depends mainly on time elapsed after the start of initiation, not on length of product RNA. , 1986, The Journal of biological chemistry.

[42]  Donald M. Crothers,et al.  Intermediates in transcription initiation from the E. coli lac UV5 promoter , 1985, Cell.

[43]  J. Gralla,et al.  Interaction of RNA polymerase with lacUV5 promoter DNA during mRNA initiation and elongation. Footprinting, methylation, and rifampicin-sensitivity changes accompanying transcription initiation. , 1985, Journal of molecular biology.

[44]  W. Reznikoff,et al.  Abortive initiation and long ribonucleic acid synthesis. , 1981, Biochemistry.

[45]  W R McClure,et al.  Role of the sigma subunit of Escherichia coli RNA polymerase in initiation. I. Characterization of core enzyme open complexes. , 1980, The Journal of biological chemistry.

[46]  M. Jacquet,et al.  Initiation, elongation and inactivation of lac messenger RNA in Escherichia coli studied by measurement of its β-galactosidase synthesizing capacity in vivo☆ , 1971 .

[47]  M. Jacquet,et al.  Initiation, elongation and inactivation of lac messenger RNA in Escherichia coli studied studied by measurement of its beta-galactosidase synthesizing capacity in vivo. , 1971, Journal of Molecular Biology.

[48]  C. Yanofsky,et al.  Tryptophan messenger ribonucleic acid elongation rates and steady-state levels of tryptophan operon enzymes under various growth conditions. , 1970, Journal of molecular biology.

[49]  R. Burgess,et al.  Cyclic Re-use of the RNA Polymerase Sigma Factor , 1969, Nature.