Ameliorating effect of the biological Zinc nanoparticles in abamectin induced hepato-renal injury in a rat model: Implication of oxidative stress, biochemical markers and COX-2 signaling pathways

Extensive use of abamectin (ABM) as an anthelmintic in veterinary systems adversely affects the health and welfare of animals and humans. Zinc nanoparticles (ZnNPs) have therapeutic benefits and ameliorate the effect of environmental pollutants. In this study, we assessed the ameliorative effect of ZnNPs against the sub-lethal toxicity of ABM in rats. Forty healthy rats were randomly selected into four groups (n = 10); the control received normal saline and test rats were treated orally twice weekly with ABM (1 mg/kg bwt), ZnNPs (10 mg/kg bwt) and ABM + ZnNPs for 28 days. Upon completion of the study period, blood and tissue samples were collected and prepared for hematological, biochemical, pathological, and immunohistochemical analysis. Our results showed that ABM treatment significantly decreased body weight gain (BWG), red blood cells (RBCs), hemoglobin (Hb), hematocrit (HC), and platelet (PLT); while it significantly increased white blood cells (WBCs) and lymphocytes. ABM also significantly decreased antioxidant enzyme activities: superoxide dismuthase (SOD), glutathione peroxidase (GPx), and catalase (CAT) and increased hydrogen peroxide and malondialdehyde levels compared with other groups. ABM significantly raised alanine aminotransferase (ALT), aspartate amino transaminase (AST), and alkaline phosphatase (ALP) levels, which was restored by co-administration of ZnNPs. Moreover, ZnNPs ameliorated ABM-mediated negative histopathological changes in the liver and kidney tissues, exhibiting a significant protective effect. Cyclooxygenase 2 (COX-2) + immuno-expression were reduced after pretreatment with ZnNPs. These findings suggested that co-administration of ZnNPs with ABM mitigated its toxicity by combating oxidative stress and boosting antioxidant capacity, indicating the efficacy of ZnNPs in attenuating ABM toxicity. Graphical Abstract

[1]  Aili Gao,et al.  Doramectin inhibits glioblastoma cell survival via regulation of autophagy in vitro and in vivo , 2022, International journal of oncology.

[2]  B. Oyinloye,et al.  Nephroprotective and anti-inflammatory potential of aqueous extract from Persea americana seeds against cadmium-induced nephrotoxicity in Wistar rats , 2021, BioMetals.

[3]  Rania S. M. Eid,et al.  Biological silicon nanoparticles improve Phaseolus vulgaris L. yield and minimize its contaminant contents on a heavy metals-contaminated saline soil. , 2021, Journal of environmental sciences.

[4]  A. El‐kott,et al.  Ephedra sinica mitigates hepatic oxidative stress and inflammation via suppressing the TLR4/MyD88/NF-κB pathway in fipronil-treated rats , 2021, Environmental Science and Pollution Research.

[5]  Synan F. AbuQamar,et al.  Selenium nanoparticles from Lactobacillus paracasei HM1 capable of antagonizing animal pathogenic fungi as a new source from human breast milk , 2021, Saudi Journal of Biological Sciences.

[6]  M. Alagawany,et al.  Nanominerals: Fabrication Methods, Benefits and Hazards, and Their Applications in Ruminants with Special Reference to Selenium and Zinc Nanoparticles , 2021, Animals : an open access journal from MDPI.

[7]  M. A. Abd El-Hack,et al.  Impacts of Supplementing Broiler Diets with Biological Curcumin, Zinc Nanoparticles and Bacillus licheniformis on Growth, Carcass Traits, Blood Indices, Meat Quality and Cecal Microbial Load , 2021, Animals : an open access journal from MDPI.

[8]  M. Moustafa,et al.  Polyphenolic extracts from pomegranate and watermelon wastes as substrate to fabricate sustainable silver nanoparticles with larvicidal effect against Spodoptera littoralis , 2021, Saudi journal of biological sciences.

[9]  E. Esimbekova,et al.  Pesticides: formulants, distribution pathways and effects on human health – a review , 2021, Toxicology reports.

[10]  M. Soliman,et al.  Impact of mycogenic zinc nanoparticles on performance, behavior, immune response, and microbial load in Oreochromis niloticus , 2021, Saudi journal of biological sciences.

[11]  M. Refat,et al.  Antioxidant, Antigenotoxic, and Hepatic Ameliorative Effects of Quercetin/Zinc Complex on Cadmium-Induced Hepatotoxicity and Alterations in Hepatic Tissue Structure , 2021, Coatings.

[12]  Amira M. El-Tahan,et al.  The use of biological selenium nanoparticles to suppress Triticum aestivum L. crown and root rot diseases induced by Fusarium species and improve yield under drought and heat stress , 2021, Saudi journal of biological sciences.

[13]  M. Alagawany,et al.  Paenibacillus polymyxa (LM31) as a new feed additive: Antioxidant and antimicrobial activity and its effects on growth, blood biochemistry, and intestinal bacterial populations of growing Japanese quail , 2021, Animal Feed Science and Technology.

[14]  M. Sitohy,et al.  Green nanotechnology for preserving and enriching yogurt with biologically available iron (II) , 2021 .

[15]  M. Alagawany,et al.  Use of biological nano zinc as a feed additive in quail nutrition: biosynthesis, antimicrobial activity and its effect on growth, feed utilisation, blood metabolites and intestinal microbiota , 2021, Italian Journal of Animal Science.

[16]  M. Alagawany,et al.  The stress of abamectin toxicity reduced water quality, growth performance, immunity and antioxidant capacity of Oreochromis niloticus fish: Modulatory role of Simmondsia chinensis extract as a dietary supplement , 2020 .

[17]  Uswatun Hasanah Zaidan,et al.  Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties , 2020, Scientific Reports.

[18]  G. Ricevuti,et al.  Ozone: a natural bioactive molecule with antioxidant property as potential new strategy in aging and in neurodegenerative disorders , 2020, Ageing Research Reviews.

[19]  Zhiqiu Huang,et al.  Abamectin at environmentally-realistic concentrations cause oxidative stress and genotoxic damage in juvenile fish (Schizothorax prenanti). , 2020, Aquatic toxicology.

[20]  H. Dahms,et al.  Mitigative effects of zinc on cadmium-induced reproductive toxicity in the male freshwater crab Sinopotamon henanense , 2020, Environmental Science and Pollution Research.

[21]  M. Fouda,et al.  Ecofriendly Synthesis and Insecticidal Application of Copper Nanoparticles against the Storage Pest Tribolium castaneum , 2020, Nanomaterials.

[22]  E. Bezirtzoglou,et al.  Environmental and Health Impacts of Air Pollution: A Review , 2020, Frontiers in Public Health.

[23]  R. Taheri,et al.  Effect of astaxanthin nanoparticles in protecting the post-thawing quality of rooster sperm challenged by cadmium administration , 2020, Poultry science.

[24]  L. Aleya,et al.  The effects of abamectin on oxidative stress and gene expression in rat liver and brain tissues: Modulation by sesame oil and ascorbic acid. , 2019, The Science of the total environment.

[25]  O. El-tawil,et al.  Nanoparticles of zinc oxide defeat chlorpyrifos-induced immunotoxic effects and histopathological alterations , 2019, Veterinary world.

[26]  N. Chandrasekaran,et al.  Safety of Natural Insecticides: Toxic Effects on Experimental Animals , 2018, BioMed research international.

[27]  E. Omara,et al.  Role of zinc oxide nanoparticles in alleviating hepatic fibrosis and nephrotoxicity induced by thioacetamide in rats. , 2018, Canadian journal of physiology and pharmacology.

[28]  Sung Ryul Lee,et al.  Critical Role of Zinc as Either an Antioxidant or a Prooxidant in Cellular Systems , 2018, Oxidative medicine and cellular longevity.

[29]  R. Pasricha,et al.  Supplementing zinc oxide nanoparticles to cryopreservation medium minimizes the freeze-thaw-induced damage to spermatozoa. , 2017, Biochemical and biophysical research communications.

[30]  S. Mansour,et al.  Zinc Ameliorate Oxidative Stress and Hormonal Disturbance Induced by Methomyl, Abamectin, and Their Mixture in Male Rats , 2017, Toxics.

[31]  F. Hassan,et al.  Growth Performance, Serum Biochemical, Economic Evaluation and IL6 Gene Expression in Growing Rabbits Fed Diets Supplemented with Zinc Nanoparticles , 2017 .

[32]  Noha M Meligi,et al.  Protective effects of Eruca sativa (rocket) on abamectin insecticide toxicity in male albino rats , 2017, Environmental Science and Pollution Research.

[33]  A. Karaduman,et al.  Identification by using MALDI-TOF mass spectrometry of lactic acid bacteria isolated from non-commercial yogurts in southern Anatolia, Turkey. , 2017, International microbiology : the official journal of the Spanish Society for Microbiology.

[34]  Ashwani Kumar Thukral,et al.  Seed pre-soaking with 24-epibrassinolide reduces the imidacloprid pesticide residues in green pods of Brassica juncea L. , 2017 .

[35]  M. Ali,et al.  Efficacy of Zinc Oxide Nanoparticles and Curcumin in Amelioration the Toxic Effects in Aflatoxicated Rabbits , 2016 .

[36]  A. H. El-Sebae,et al.  Hepatotoxicity and Nephrotoxicity in Mice Induced by Abamectin and Ameliorating Effect of Quercetin , 2015 .

[37]  M. Daam,et al.  Toxicity of Vertimec® 18 EC (active ingredient abamectin) to the neotropical cladoceran Ceriodaphnia silvestrii. , 2015, Chemosphere.

[38]  S. Hewitt,et al.  Controls for Immunohistochemistry , 2014, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[39]  A. Prasad.  Zinc is an Antioxidant and Anti-Inflammatory Agent: Its Role in Human Health , 2014, Front. Nutr..

[40]  M. Kesmati,et al.  Effects of nano and conventional Zinc Oxide on anxiety-like behavior in male rats , 2013, Indian journal of pharmacology.

[41]  F. Gentile,et al.  Interaction of aldehydes derived from lipid peroxidation and membrane proteins , 2013, Front. Physiol..

[42]  Hany Abdelhady,et al.  Abamectin Induced Biochemical and Histopathological Changes in the Albino Rat, Rattus Norvegicus , 2013 .

[43]  F. Arechavaleta-Velasco,et al.  Differential Effect of DDT, DDE, and DDD on COX‐2 Expression in the Human Trophoblast Derived HTR‐8/SVneo Cells , 2012, Journal of biochemical and molecular toxicology.

[44]  Ja Gilbertson,et al.  Bancroft's Theory and Practice of Histological Techniques , 2012 .

[45]  Xiaolong Wang,et al.  Effects of atrazine and chlorpyrifos on the mRNA levels of IL-1 and IFN-γ2b in immune organs of common carp. , 2011, Fish & shellfish immunology.

[46]  M. Adzic,et al.  Pulmonary, Gastrointestinal and Urogenital Pharmacology Fluoxetine affects antioxidant system and promotes apoptotic signaling in Wistar rat liver , 2011 .

[47]  N. El-Shenawy,et al.  Effects of insecticides fenitrothion, endosulfan and abamectin on antioxidant parameters of isolated rat hepatocytes. , 2010, Toxicology in vitro : an international journal published in association with BIBRA.

[48]  F. Qadri,et al.  Zinc influences innate immune responses in children with enterotoxigenic Escherichia coli-induced diarrhea. , 2010, The Journal of nutrition.

[49]  N. Zidan,et al.  Haematological, Biochemical and Histopathological Alterations Induced by Abamectin and Bacillus Thuringiensis in Male Albino Rats , 2010, Acta biologica Hungarica.

[50]  M. Hejazi,et al.  Isolation and phenotypic characterization of Lactobacillus species from various dairy products. , 2010 .

[51]  Jing Kong,et al.  Superwetting nanowire membranes for selective absorption. , 2008, Nature nanotechnology.

[52]  S. Ōmura.  Ivermectin: 25 years and still going strong. , 2008, International journal of antimicrobial agents.

[53]  I. Leclercq,et al.  COX‐2 induction in mice with experimental nutritional steatohepatitis: Role as pro‐inflammatory mediator , 2006, Hepatology.

[54]  V. Jenčič,et al.  Abamectin effects on rainbow trout (Oncorhynchus mykiss) , 2006, Ecotoxicology.

[55]  Steven A. Bailey,et al.  Relationships Between Organ Weight and Body/Brain Weight in the Rat: What Is the Best Analytical Endpoint? , 2004, Toxicologic pathology.

[56]  Y. Yoon,et al.  Avermectin: biochemical and molecular basis of its biosynthesis and regulation , 2004, Applied Microbiology and Biotechnology.

[57]  M. Panemangalore,et al.  Exposure to Low Doses of Endosulfan and Chlorpyrifos Modifies Endogenous Antioxidants in Tissues of Rats , 2003, Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes.

[58]  M. Abdollahi,et al.  Induction of oxidative stress and acetylcholinesterase inhibition in organophosphorous pesticide manufacturing workers , 2002, Human & experimental toxicology.

[59]  Y. Surh,et al.  Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. , 2001, Mutation research.

[60]  S. Yasar,et al.  In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats , 2001, Archives of Toxicology.

[61]  C. Packman.  The spherocytic Haemolytic Anaemias , 2001, British journal of haematology.

[62]  C. Shaha,et al.  Role of glutathione S-transferases in oxidative stress-induced male germ cell apoptosis. , 2000, Free radical biology & medicine.

[63]  M. E. Hahn,et al.  The evolution of aryl hydrocarbon signaling proteins: diversity of ARNT isoforms among fish species. , 2000, Marine environmental research.

[64]  J. Cerón,et al.  Cholinesterase activity and hematological parameters as biomarkers of sublethal molinate exposure in Anguilla anguilla. , 2000, Ecotoxicology and environmental safety.

[65]  R. Herd,et al.  Endectocidal drugs: ecological risks and counter-measures. , 1995, International journal for parasitology.

[66]  B. H. Ali.  THE EFFECT OF IVERMECTIN ON SOME HAEMATOLOGICAL INDICES IN RABBITS: INFLUENCE OF VITAMIN K TREATMENT , 1990, Clinical and experimental pharmacology & physiology.

[67]  S. Hsu,et al.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. , 1981, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[68]  E. Pick,et al.  A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. , 1980, Journal of immunological methods.

[69]  K. Yagi,et al.  Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. , 1979, Analytical biochemistry.

[70]  C. Harper.  Wernicke's encephalopathy: a more common disease than realised. A neuropathological study of 51 cases. , 1979, Journal of neurology, neurosurgery, and psychiatry.

[71]  K. Yagi,et al.  The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. , 1972, Biochemical and biophysical research communications.

[72]  G. Ellis,et al.  Colorimetric determination of serum acid phosphatase activity using adenosine 3′-monophosphate as substrate , 1971, Journal of clinical pathology.

[73]  W. Valentine,et al.  Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. , 1967, The Journal of laboratory and clinical medicine.

[74]  S REITMAN,et al.  A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. , 1957, American journal of clinical pathology.

[75]  S. Mahgoub,et al.  Biosynthesis, Optimization and Characterization of Silver Nanoparticles Using a Soil Isolate of Bacillus pseudomycoides MT32 and their Antifungal Activity Against some Pathogenic Fungi , 2019, Advances in Animal and Veterinary Sciences.

[76]  Jigang Wu,et al.  Cytotoxic effects of Avermectin on human HepG2 cells in vitro bioassays. , 2017, Environmental pollution.

[77]  H. Nasr,et al.  Neuro and renal toxicity induced by chlorpyrifos and abamectin in rats , 2015, Environmental Science and Pollution Research.

[78]  M. Lhermitte,et al.  Effect of sub-acute exposure to abamectin “insecticide” on liver rats (Rattus norvegicus) , 2013 .

[79]  S. Mansour,et al.  Oxidative damage, biochemical and histopathological alterations in rats exposed to chlorpyrifos and the antioxidant role of zinc , 2010 .

[80]  C. A. V. van Gestel,et al.  Toxicity of abamectin and doramectin to soil invertebrates. , 2008, Environmental pollution.

[81]  Isaac Ishaaya,et al.  Biochemical Sites of Insecticide Action and Resistance , 2001, Springer Berlin Heidelberg.

[82]  E. Stapley,et al.  Isolation and Characterization of the Producing Organism , 1989 .

[83]  H. Aebi,et al.  Catalase in vitro. , 1984, Methods in enzymology.