Effect of Oxygen Tension on the Developmental Potential of Parthenogenetic Oocytes and Nuclear-Transferred Porcine Oocytes Receiving Fetal Fibroblast Cells

The effects of oxygen tension (5% and 20%) during in vitro culture of oocytes and fetal fibroblast cells on the developmental potential of parthenogenetic and nuclear-transferred porcine oocytes were examined. The potential of parthenogenetic oocytes matured and cultured under different oxygen tensions to develop into blastocysts was not significantly different (5 to 16%). The proportions of enucleated oocytes receiving fetal fibroblast cells that developed into blastocysts were significantly lower when somatic cells were cultured under low oxygen tension (2 and 3%) than under high oxygen tension (7%). The effects of oxygen tension during culture of oocytes and somatic cells on the developmental potential of parthenogenetic and nuclear-transferred oocytes is discussed.

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