Pineal opsin: a nonvisual opsin expressed in chick pineal

Pineal opsin (P-opsin), an opsin from chick that is highly expressed in pineal but is not detectable in retina, was cloned by the polymerase chain reaction. It is likely that the P-opsin lineage diverged from the retinal opsins early in opsin evolution. The amino acid sequence of P-opsin is 42 to 46 percent identical to that of the retinal opsins. P-opsin is a seven-membrane spanning, G protein-linked receptor with a Schiff-base lysine in the seventh membrane span and a Schiff-base counterion in the third membrane span. The primary sequence of P-opsin suggests that it will be maximally sensitive to approximately 500-nanometer light and produce a slow and prolonged phototransduction response consistent with the nonvisual function of pineal photoreception.

[1]  Toshiyuki Okano,et al.  Pinopsin is a chicken pineal photoreceptive molecule , 1994, Nature.

[2]  J B Hurley,et al.  Transduction mechanisms of vertebrate and invertebrate photoreceptors. , 1994, The Journal of biological chemistry.

[3]  M. Zatz.  Photoendocrine Transduction in Cultured Chick Pineal Cells: IV. What Do Vitamin A Depletion and Retinaldehyde Addition Do to the Effects of Light on the Melatonin Rhythm? , 1994, Journal of neurochemistry.

[4]  H. Khorana,et al.  Structure and function in rhodopsin: replacement by alanine of cysteine residues 110 and 187, components of a conserved disulfide bond in rhodopsin, affects the light-activated metarhodopsin II state. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[5]  McLaughlin Sk,et al.  33P is preferable to 35S for labeling probes used in In situ hybridization , 1993 .

[6]  C. Zuker,et al.  Arrestin function in inactivation of G protein-coupled receptor rhodopsin in vivo. , 1993, Science.

[7]  H. Khorana,et al.  Structure and function in rhodopsin. Studies of the interaction between the rhodopsin cytoplasmic domain and transducin. , 1992, The Journal of biological chemistry.

[8]  Y. Fukada,et al.  Primary structures of chicken cone visual pigments: vertebrate rhodopsins have evolved out of cone visual pigments. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[9]  R. Margolskee,et al.  Gustducin is a taste-cell-specific G protein closely related to the transducins , 1992, Nature.

[10]  J Nathans,et al.  A visual pigment from chicken that resembles rhodopsin: amino acid sequence, gene structure, and functional expression. , 1992, Biochemistry.

[11]  J. Mollon,et al.  Sequence divergence and copy number of the middle- and long-wave photopigment genes in old world monkeys , 1992, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[12]  Y. Fukada,et al.  Differentiation of both rod and cone types of photoreceptors in the in vivo and in vitro developing pineal glands of the quail. , 1992, Brain research. Developmental brain research.

[13]  H. Khorana.  Rhodopsin, photoreceptor of the rod cell. An emerging pattern for structure and function. , 1992, The Journal of biological chemistry.

[14]  G D Schuler,et al.  A workbench for multiple alignment construction and analysis , 1991, Proteins.

[15]  J. Flannery,et al.  Uptake, processing and release of retinoids by cultured human retinal pigment epithelium. , 1990, Experimental eye research.

[16]  S. Yokoyama,et al.  Convergent evolution of the red- and green-like visual pigment genes in fish, Astyanax fasciatus, and human. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[17]  Elliott M. Ross,et al.  Signal sorting and amplification through G protein-coupled receptors , 1989, Neuron.

[18]  J. Takahashi,et al.  The avian pineal, a vertebrate model system of the circadian oscillator: cellular regulation of circadian rhythms by light, second messengers, and macromolecular synthesis. , 1989, Recent progress in hormone research.

[19]  M. Zatz,et al.  Two mechanisms of photoendocrine transduction in cultured chick pineal cells: pertussis toxin blocks the acute but not the phase-shifting effects of light on the melatonin rhythm , 1988, Brain Research.

[20]  J. Takahashi,et al.  Circadian clock in cell culture: I. Oscillation of melatonin release from dissociated chick pineal cells in flow-through microcarrier culture , 1988, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[21]  J. Takahashi,et al.  Circadian clock in cell culture: II. In vitro photic entrainment of melatonin oscillation from dissociated chick pineal cells , 1988, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[22]  J. Felsenstein.  Phylogenies from molecular sequences: inference and reliability. , 1988, Annual review of genetics.

[23]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[24]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[25]  D. Melton,et al.  In vitro RNA synthesis with SP6 RNA polymerase. , 1987, Methods in enzymology.

[26]  J. Nathans,et al.  Molecular genetics of human color vision: the genes encoding blue, green, and red pigments. , 1986, Science.

[27]  S. W. Hall,et al.  Phosphodiesterase activation by photoexcited rhodopsin is quenched when rhodopsin is phosphorylated and binds the intrinsic 48-kDa protein of rod outer segments. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[28]  J. Felsenstein.  CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP , 1985, Evolution; international journal of organic evolution.

[29]  G. Church,et al.  Genomic sequencing. , 1993, Methods in molecular biology.

[30]  J. Devereux,et al.  A comprehensive set of sequence analysis programs for the VAX , 1984, Nucleic Acids Res..

[31]  H. Kühn.  Chapter 5 Interactions between photoexcited rhodopsin and light-activated enzymes in rods , 1984 .

[32]  R. Doolittle,et al.  A simple method for displaying the hydropathic character of a protein. , 1982, Journal of molecular biology.

[33]  T. Deguchi.  Rhodopsin-like photosensitivity of isolated chicken pineal gland , 1981, Nature.

[34]  T. Deguchi.  Circadian rhythm of serotonin N-acetyltransferase activity in organ culture of chicken pineal gland. , 1979, Science.