Forces and Bond Dynamics in Cell Adhesion

Adhesion of a biological cell to another cell or the extracellular matrix involves complex couplings between cell biochemistry, structural mechanics, and surface bonding. The interactions are dynamic and act through association and dissociation of bonds between very large molecules at rates that change considerably under stress. Combining molecular cell biology with single-molecule force spectroscopy provides a powerful tool for exploring the complexity of cell adhesion, that is, how cell signaling processes strengthen adhesion bonds and how forces applied to cell-surface bonds act on intracellular sites to catalyze chemical processes or switch molecular interactions on and off. Probing adhesion receptors on strategically engineered cells with force during functional stimulation can reveal key nodes of communication between the mechanical and chemical circuitry of a cell.

[1]  Kenneth M. Yamada,et al.  Transmembrane crosstalk between the extracellular matrix and the cytoskeleton , 2001, Nature Reviews Molecular Cell Biology.

[2]  David R Critchley,et al.  The structure and regulation of vinculin. , 2006, Trends in cell biology.

[3]  K. Schulten,et al.  Single-Molecule Experiments in Vitro and in Silico , 2007, Science.

[4]  M. Arnaout,et al.  Integrin structure, allostery, and bidirectional signaling. , 2005, Annual review of cell and developmental biology.

[5]  A. Chadli THE CANCER CELL , 1924, La Presse medicale.

[6]  Michael P. Sheetz,et al.  Two-piconewton slip bond between fibronectin and the cytoskeleton depends on talin , 2003, Nature.

[7]  Richard O Hynes,et al.  Integrins Bidirectional, Allosteric Signaling Machines , 2002, Cell.

[8]  David A Calderwood,et al.  Integrin β cytoplasmic domain interactions with phosphotyrosine-binding domains: A structural prototype for diversity in integrin signaling , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[9]  Michael P. Sheetz,et al.  Force Sensing by Mechanical Extension of the Src Family Kinase Substrate p130Cas , 2006, Cell.

[10]  David L Stokes,et al.  Untangling Desmosomal Knots with Electron Tomography , 2003, Science.

[11]  Brian P Helmke,et al.  Mechanisms of mechanotransduction. , 2006, Developmental cell.

[12]  R. Liddington,et al.  Structural determinants of integrin recognition by talin. , 2003, Molecular cell.

[13]  M. Davis,et al.  A receptor/cytoskeletal movement triggered by costimulation during T cell activation. , 1998, Science.

[14]  E. Evans Probing the relation between force--lifetime--and chemistry in single molecular bonds. , 2001, Annual review of biophysics and biomolecular structure.

[15]  S. Sen,et al.  Matrix Elasticity Directs Stem Cell Lineage Specification , 2006, Cell.

[16]  Claire M Brown,et al.  Probing the integrin-actin linkage using high-resolution protein velocity mapping , 2006, Journal of Cell Science.

[17]  Gaudenz Danuser,et al.  Differential Transmission of Actin Motion Within Focal Adhesions , 2007, Science.

[18]  P. Sperryn,et al.  Blood. , 1989, British journal of sports medicine.

[19]  C. Carman,et al.  Integrin avidity regulation: are changes in affinity and conformation underemphasized? , 2003, Current opinion in cell biology.

[20]  L. Wilkinson Immunity , 1891, The Lancet.

[21]  Evan Evans,et al.  Nano- to microscale dynamics of P-selectin detachment from leukocyte interfaces. I. Membrane separation from the cytoskeleton. , 2005, Biophysical journal.

[22]  R. McEver Selectins: lectins that initiate cell adhesion under flow. , 2002, Current opinion in cell biology.

[23]  Benjamin Geiger,et al.  Exploring the Neighborhood Adhesion-Coupled Cell Mechanosensors , 2002, Cell.

[24]  M. Sixt,et al.  Beta1 integrins: zip codes and signaling relay for blood cells. , 2006, Current opinion in cell biology.

[25]  Deborah Leckband,et al.  Mechanism and dynamics of cadherin adhesion. , 2006, Annual review of biomedical engineering.

[26]  D. Leckband,et al.  Lifetime measurements reveal kinetic differences between homophilic cadherin bonds. , 2006, Biophysical journal.

[27]  T. Springer,et al.  Integrin structures and conformational signaling. , 2006, Current opinion in cell biology.

[28]  E. Evans,et al.  Nano- to microscale dynamics of P-selectin detachment from leukocyte interfaces. II. Tether flow terminated by P-selectin dissociation from PSGL-1. , 2005, Biophysical journal.

[29]  Cynthia A. Reinhart-King,et al.  Tensional homeostasis and the malignant phenotype. , 2005, Cancer cell.

[30]  M. Ginsberg,et al.  Talin forges the links between integrins and actin , 2003, Nature Cell Biology.

[31]  I. Campbell,et al.  The molecular basis of filamin binding to integrins and competition with talin. , 2006, Molecular cell.

[32]  Evan Evans,et al.  Nano-to-micro scale dynamics of P-selectin detachment from leukocyte interfaces. III. Numerical simulation of tethering under flow. , 2005, Biophysical journal.

[33]  R. Liddington,et al.  Talin Binding to Integrin ß Tails: A Final Common Step in Integrin Activation , 2003, Science.

[34]  Grégory Giannone,et al.  Substrate rigidity and force define form through tyrosine phosphatase and kinase pathways. , 2006, Trends in cell biology.

[35]  宁北芳,et al.  疟原虫var基因转换速率变化导致抗原变异[英]/Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A , 2005 .

[36]  Michael P. Sheetz,et al.  Talin1 is critical for force-dependent reinforcement of initial integrin–cytoskeleton bonds but not tyrosine kinase activation , 2003, The Journal of cell biology.

[37]  B. Gumbiner,et al.  Crosstalk between different adhesion molecules. , 2006, Current opinion in cell biology.

[38]  Cheng Zhu,et al.  Mechanical switching and coupling between two dissociation pathways in a P-selectin adhesion bond. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[39]  Cheng Zhu,et al.  Direct observation of catch bonds involving cell-adhesion molecules , 2003, Nature.

[40]  B. Gumbiner,et al.  Regulation of cadherin-mediated adhesion in morphogenesis , 2005, Nature Reviews Molecular Cell Biology.

[41]  R. Rosenfeld Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[42]  E. Evans,et al.  Dynamic strength of molecular adhesion bonds. , 1997, Biophysical journal.

[43]  R. Tsien,et al.  The Fluorescent Toolbox for Assessing Protein Location and Function , 2006, Science.

[44]  M. Sheetz,et al.  Local force and geometry sensing regulate cell functions , 2006, Nature Reviews Molecular Cell Biology.

[45]  M. Ginsberg,et al.  Integrin cytoplasmic domain-binding proteins. , 2000, Journal of cell science.

[46]  E. Evans,et al.  Trans-bonded pairs of E-cadherin exhibit a remarkable hierarchy of mechanical strengths. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[47]  G. I. Bell Models for the specific adhesion of cells to cells. , 1978, Science.

[48]  David A. Schultz,et al.  A mechanosensory complex that mediates the endothelial cell response to fluid shear stress , 2005, Nature.

[49]  Michael Loran Dustin,et al.  Force as a facilitator of integrin conformational changes during leukocyte arrest on blood vessels and antigen-presenting cells. , 2007, Immunity.