Maturity of human bone estimated by FTIR spectroscopy analysis: implications for ostheoporosis

This work studies the possible variations of the properties of mineral and organic bone components with regard to the anatomical position and the patient's age. Autopsies of healthy human iliac crest have been analyzed within a wide range of ages (26-88), measuring different anatomical positions in trabecular bone by means of FT-IR spectroscopy. The study was focused on the analysis of ν1, ν3 phosphate, ν2 carbonate amida I and amida II bands. From the resulting spectra the cristallinity/maturity index, the collagen cross-links ratio and the carbonate/phosphate ratio were calculated. All of them provide information of bone mineral and collagen maturity. The results show a trend in the spatial distribution of mineral and collagen maturity in most of the samples. The most mature mineral and collagen of the bone were found to be located in the trabecular center, while the youngest were situated in the peripheral regions. However, this behavior has exceptions that seem to be related with the patient's age.

[1]  A. Boskey,et al.  Bone Fragility and Collagen Cross‐Links , 2004, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[2]  C. Turner.  Biomechanics of Bone: Determinants of Skeletal Fragility and Bone Quality , 2002, Osteoporosis International.

[3]  M. Chance,et al.  In situ analysis of mineral content and crystallinity in bone using infrared micro-spectroscopy of the nu(4) PO(4)(3-) vibration. , 2001, Biochimica et biophysica acta.

[4]  A. Boskey,et al.  FTIR Microspectroscopic Analysis of Human Iliac Crest Biopsies from Untreated Osteoporotic Bone , 1997, Calcified Tissue International.

[5]  Himadri S. Gupta,et al.  Structure and mechanical quality of the collagen–mineral nano-composite in bone , 2004 .

[6]  D. Donley,et al.  Bone mineral and collagen quality in iliac crest biopsies of patients given teriparatide: new results from the fracture prevention trial. , 2005, The Journal of clinical endocrinology and metabolism.

[7]  R Mendelsohn,et al.  FTIR microscopic imaging of collagen and proteoglycan in bovine cartilage. , 2001, Biopolymers.

[8]  R. Recker,et al.  Distribution of Collagen Cross‐Links in Normal Human Trabecular Bone , 2003, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[9]  Georges Boivin,et al.  Infrared imaging of calcified tissue in bone biopsies from adults with osteomalacia. , 2005, Bone.

[10]  R Mendelsohn,et al.  Spectroscopic Characterization of Collagen Cross‐Links in Bone , 2001, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[11]  A. Boskey,et al.  FTIR Microspectroscopic Analysis of Normal Human Cortical and Trabecular Bone , 1997, Calcified Tissue International.

[12]  R. Mendelsohn,et al.  Infrared Microscopic Imaging of Bone: Spatial Distribution of CO32− , 2001, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.