Purification of aldehyde oxidase from bovine ciliary body.

Ocular aldehyde oxidase was purified for the first time from bovine ciliary body cytosol by ammonium sulfate fractionation and successive HPLC using DEAE anion-exchange and hydroxyapatite columns. The purified enzyme was homogeneous by the criterion of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular weight of the enzyme was estimated to be about 150,000 by electrophoresis and to be about 300,000 by gel filtration HPLC on a TSK gel G3000SWXL column, indicating that the enzyme consists of two subunits with the same molecular weight. On the other hand, nicotinamide N-oxide reductase activity was associated with aldehyde oxidase activity throughout the purification steps of the latter enzyme. This fact indicated that nicotinamide N-oxide reductase activity of the ciliary body cytosol is due to aldehyde oxidase present in the tissue preparation.

[1]  S. Kitamura,et al.  Metabolism of drugs in the eye. Drug-reducing activity of preparations from bovine ciliary body. , 1988, Current eye research.

[2]  S. Kitamura,et al.  Nicotinamide N-oxide reductase activity in bovine and rabbit eyes. , 1987, Investigative ophthalmology & visual science.

[3]  K. Sugihara,et al.  Participation of liver aldehyde oxidase in reductive metabolism of hydroxamic acids to amides. , 1986, Archives of biochemistry and biophysics.

[4]  S. Kitamura,et al.  Reductive metabolism of aromatic nitro compounds including carcinogens by rabbit liver preparations. , 1986, Cancer research.

[5]  K. Tatsumi,et al.  Guinea pig liver aldehyde oxidase as a sulfoxide reductase: its purification and characterization. , 1985, Archives of biochemistry and biophysics.

[6]  S. Kitamura,et al.  Reduction of tertiary amine N-oxides by liver preparations: function of aldehyde oxidase as a major N-oxide reductase. , 1984, Biochemical and biophysical research communications.

[7]  S. Kitamura,et al.  Involvement of liver aldehyde oxidase in the reduction of nicotinamide N-oxide. , 1984, Biochemical and biophysical research communications.

[8]  S. Kitamura,et al.  Reductive metabolism of N-nitrosodiphenylamine to the corresponding hydrazine derivative. , 1983, Archives of biochemistry and biophysics.

[9]  S. Kitamura,et al.  Evidence for reduction of hydroxamic acids to the corresponding amides by liver aldehyde oxidase. , 1983, Chemical & pharmaceutical bulletin.

[10]  S. Kitamura,et al.  Sulfoxide reductase activity of liver aldehyde oxidase. , 1983, Biochimica et biophysica acta.

[11]  S. Kitamura,et al.  Azoreductase activity of liver aldehyde oxidase. , 1983, Chemical & pharmaceutical bulletin.

[12]  S. Kitamura,et al.  Involvement of liver aldehyde oxidase in conversion of N-hydroxyurethane to urethane. , 1983, Journal of pharmacobio-dynamics.

[13]  S. Sakiyama,et al.  Purification and characterization of N1-methylnicotinamide oxidases I and II separated from rat liver. , 1983, Archives of biochemistry and biophysics.

[14]  S. Kitamura,et al.  Rabbit liver enzymes responsible for reduction of nitropolycyclic aromatic hydrocarbons. , 1983, Chemical & pharmaceutical bulletin.

[15]  S. Kitamura,et al.  Involvement of liver aldehyde oxidase in sulfoxide reduction. , 1982, Chemical & pharmaceutical bulletin.

[16]  K. Rajagopalan,et al.  Properties of the prosthetic groups of rabbit liver aldehyde oxidase: a comparison of molybdenum hydroxylase enzymes. , 1982, Biochemistry.

[17]  H. Shichi,et al.  Enzymes of mercapturate synthesis and other drug-metabolizing reactions-specific localization in the eye. , 1981, Experimental eye research.

[18]  K. Rajagopalan,et al.  Mechanisms of inactivation of molybdoenzymes by cyanide. , 1980, The Journal of biological chemistry.

[19]  V. Massey,et al.  Preparation of aldehyde oxidase in its native and deflavo forms. Comparison of spectroscopic and catalytic properties. , 1974, The Journal of biological chemistry.

[20]  V. Massey,et al.  Evidence for an active site persulfide residue in rabbit liver aldehyde oxidase. , 1974, The Journal of biological chemistry.

[21]  R. L. Felsted,et al.  Purification and properties of the aldehyde oxidases from hog and rabbit livers. , 1973, The Journal of biological chemistry.

[22]  P. Handler,et al.  Preparation of bovine xanthine oxidase and the subunit structures of some iron flavoproteins. , 1968, The Journal of biological chemistry.

[23]  K. Rajagopalan,et al.  HEPATIC ALDEHYDE OXIDASE. 3. THE SUBSTRATE-BINDING SITE. , 1964, The Journal of biological chemistry.

[24]  K. Rajagopalan,et al.  HEPATIC ALDEHYDE OXIDASE. II. DIFFERENTIAL INHIBITION OF ELECTRON TRANSFER TO VARIOUS ELECTRON ACCEPTORS. , 1964, The Journal of biological chemistry.

[25]  I. Fridovich,et al.  Hepatic aldehyde oxidase. I. Purification and properties. , 1962, The Journal of biological chemistry.

[26]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.