Development of a hybrid Raman spectroscopy and optical coherence tomography technique for real-time in vivo tissue measurements.

We report on the development of a unique sideview handheld hybrid Raman spectroscopy (RS) and optical coherence tomography (OCT) technique for real-time in vivo tissue measurements. A sideview handheld RS-OCT optical probe is designed to coalign the optical paths of RS and OCT sampling arms, whereby a compact long-pass dichroic mirror (LPDM) is utilized to transmit the OCT signal through a gradient index rod lens and a reflection mirror, whereas the LPDM deflects the tissue Raman signal by 90°, leading to coaligned RS/OCT optical samplings on the tissue. Further study shows that the hybrid RS and OCT technique developed is capable of simultaneously acquiring both morphological and biochemical information about the oral tissue in vivo, facilitating real-time, in vivo tissue diagnoses and characterizations in the oral cavity.

[1]  Mads S. Bergholt,et al.  Characterizing variability of in vivo Raman spectroscopic properties of different anatomical sites of normal colorectal tissue towards cancer diagnosis at colonoscopy. , 2015, Analytical chemistry.

[2]  F. McNeillie,et al.  The imaging properties of gradient index optical fibres , 2004 .

[3]  Khek Yu Ho,et al.  Integrated Raman spectroscopy and trimodal wide-field imaging techniques for real-time in vivo tissue Raman measurements at endoscopy. , 2009, Optics letters.

[4]  Mads S. Bergholt,et al.  Characterizing variability in in vivo Raman spectroscopic properties of different anatomical sites of normal tissue in the oral cavity , 2012 .

[5]  Mads S. Bergholt,et al.  Simultaneous fingerprint and high‐wavenumber fiber‐optic Raman spectroscopy enhances real‐time in vivo diagnosis of adenomatous polyps during colonoscopy , 2016, Journal of biophotonics.

[6]  K Divakar Rao,et al.  Depth-sensitive Raman spectroscopy combined with optical coherence tomography for layered tissue analysis. , 2014, Journal of biophotonics.

[7]  Matthew D. Keller,et al.  Combined Raman spectroscopy and optical coherence tomography device for tissue characterization. , 2008, Optics letters.

[8]  D I McLean,et al.  Rapid near-infrared Raman spectroscopy system for real-time in vivo skin measurements. , 2001, Optics letters.

[9]  Bahar Davoudi,et al.  Noninvasive in vivo structural and vascular imaging of human oral tissues with spectral domain optical coherence tomography , 2012, Biomedical optics express.

[10]  Daniel Fried,et al.  Imaging caries lesions and lesion progression with polarization-sensitive optical coherence tomography , 2002, SPIE BiOS.

[11]  Khek Yu Ho,et al.  Rapid Fiber-optic Raman Spectroscopy for Real-Time In Vivo Detection of Gastric Intestinal Metaplasia during Clinical Gastroscopy , 2016, Cancer Prevention Research.

[12]  Anita Mahadevan-Jansen,et al.  A clinical instrument for combined raman spectroscopy‐optical coherence tomography of skin cancers , 2011, Lasers in surgery and medicine.

[13]  J. Fujimoto,et al.  Optical Coherence Tomography , 1991 .

[14]  V A Parsegian,et al.  Raman spectral evidence for hydration forces between collagen triple helices. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[15]  G. Gelikonov,et al.  In vivo OCT imaging of hard and soft tissue of the oral cavity. , 1998, Optics express.

[16]  H. Tsuda,et al.  Raman Spectroscopy in Dental Research: A Short Review of Recent Studies , 1997, Advances in dental research.

[17]  Wei Zheng,et al.  Optical diagnosis and characterization of dental caries with polarization-resolved hyperspectral stimulated Raman scattering microscopy. , 2016, Biomedical optics express.

[18]  Wei Zheng,et al.  Polarization-resolved hyperspectral stimulated Raman scattering microscopy for label-free biomolecular imaging of the tooth , 2016 .

[19]  K. S. Krishnan,et al.  A New Type of Secondary Radiation , 1928, Nature.

[20]  Peter Williams,et al.  Ex vivo detection and characterization of early dental caries by optical coherence tomography and Raman spectroscopy. , 2005, Journal of biomedical optics.

[21]  M. Teh,et al.  Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy improves in vivo diagnosis of esophageal squamous cell carcinoma at endoscopy , 2015, Scientific Reports.

[22]  J. Fujimoto,et al.  High-precision, high-accuracy ultralong-range swept-source optical coherence tomography using vertical cavity surface emitting laser light source. , 2013, Optics letters.

[23]  Mads S. Bergholt,et al.  Development of a beveled fiber-optic confocal Raman probe for enhancing in vivo epithelial tissue Raman measurements at endoscopy. , 2013, Optics letters.