Headspace solid-phase microextraction (HS-SPME) and liquid-liquid extraction (LLE): comparison of the performance in classification of ecstasy tablets. Part 2.

Headspace solid-phase microextraction (HS-SPME) is assessed as an alternative to liquid-liquid extraction (LLE) currently used for 3,4-methylenedioxymethampethamine (MDMA) profiling. Both methods were compared evaluating their performance in discriminating and classifying samples. For this purpose 62 different seizures were analysed using both extraction techniques followed by gas chromatography-mass spectroscopy (GC-MS). A previously validated method provided data for HS-SPME, whereas LLE data were collected applying a harmonized methodology developed and used in the European project CHAMP. After suitable pre-treatment, similarities between sample pairs were studied using the Pearson correlation. Both methods enable to distinguish between samples coming from the same pre-tabletting batches and samples coming from different pre-tabletting batches. This finding emphasizes the use of HS-SPME as an effective alternative to LLE, with additional advantages such as sample preparation and a solvent-free process.

[1]  C. Koester,et al.  Optimum methamphetamine profiling with sample preparation by solid-phase microextraction. , 2002, Journal of forensic sciences.

[2]  P Hayoz,et al.  Establishment of an operational system for drug profiling: a Swiss experience. , 2005, Bulletin on narcotics.

[3]  Hiroyuki Inoue,et al.  Comparison and classification of methamphetamine seized in Japan and Thailand using gas chromatography with liquid-liquid extraction and solid-phase microextraction. , 2008, Forensic science international.

[4]  Fabien Palhol,et al.  Impurity profiling of seized MDMA tablets by capillary gas chromatography , 2002, Analytical and bioanalytical chemistry.

[5]  J. Coumbaros,et al.  Application of solid-phase microextraction to the profiling of an illicit drug: manufacturing impurities in illicit 4-methoxyamphetamine. , 1999, Journal of forensic sciences.

[6]  Michael D Cole,et al.  Development of a harmonised method for the profiling of amphetamines: IV. Optimisation of sample preparation. , 2007, Forensic science international.

[7]  M M van Deursen,et al.  Organic impurity profiling of 3,4-methylenedioxymethamphetamine (MDMA) tablets seized in The Netherlands. , 2006, Science & justice : journal of the Forensic Science Society.

[8]  J. Almirall,et al.  Analysis of gamma-hydroxybutyric acid (GHB) in spiked water and beverage samples using solid phase microextraction (SPME) on fiber derivatization/gas chromatography-mass spectrometry (GC/MS). , 2005, Journal of forensic sciences.

[9]  L. Dujourdy,et al.  A study of impurities in intermediates and 3,4-methylenedioxymethamphetamine (MDMA) samples produced via reductive amination routes. , 2005, Forensic science international.

[10]  Stacy D. Brown,et al.  A validated SPME-GC-MS method for simultaneous quantification of club drugs in human urine. , 2007, Forensic science international.

[11]  Laura Aalberg,et al.  Drug intelligence based on MDMA tablets data I. Organic impurities profiling. , 2008, Forensic science international.

[12]  Tom Fawcett,et al.  An introduction to ROC analysis , 2006, Pattern Recognit. Lett..

[13]  F Taroni,et al.  A methodology for illicit heroin seizures comparison in a drug intelligence perspective using large databases. , 2003, Forensic science international.

[14]  Pierre Margot,et al.  Optimization of HS-SPME/GC-MS analysis and its use in the profiling of illicit ecstasy tablets (Part 1). , 2009, Forensic science international.

[15]  Laura Aalberg,et al.  Drug intelligence based on MDMA tablets data: 2. Physical characteristics profiling. , 2008, Forensic science international.

[16]  Hiroyuki Inoue,et al.  Identification of impurities and the statistical classification of methamphetamine using headspace solid phase microextraction and gas chromatography-mass spectrometry. , 2006, Forensic science international.