Alkaline hydrolysis behavior of metal-organic frameworks NH2-MIL-53(Al) employed for sensitive immunoassay via releasing fluorescent molecules.

Nano-sized metal-organic frameworks (MOFs) NH2-MIL-53(Al) were synthesized from 2-aminoterephthalic acid (NH2·H2BDC) and AlCl3 by a facile hydrothermal method. The synthesized MOFs displayed good stability and uniform particle size in netural medium, and hydrolyzed in alkaline medium to release a large amount of fluorescent ligand NH2·H2BDC. Therefore they can act as large-capability nanovehicles to load signal molecules for investigating various biorecognition events. In this work, based on the alkaline hydrolysis behavior of MOFs NH2-MIL-53(Al), a sensitive immunoassay method was developed for the detection of aflatoxin B1 (AFB1) by employing they as the fluorescent signal probes. With a competitive immunoassay mode on microplate, AFB1 can be detected within a linear range of 0.05 ng mL-1 - 25 ng mL-1. The method was successfully employed to detect AFB1 spiked in Job Tears, Polygala Tenuifolia and with acceptable recovery values of 83.00% - 114.00%. The detection results for moldy Fructus Xanthii displayed acceptable agreement with those by HPLC method, with relative errors of -14.21% - 3.49%. With the merits of high sensitivity, facile manipulation and ideal reliability, the approach can also be extended to other areas such as aptasensor and receptor binding assay.

[1]  P. Bermejo-Barrera,et al.  Aflatoxins screening in non-dairy beverages by Mn-doped ZnS quantum dots - Molecularly imprinted polymer fluorescent probe. , 2019, Talanta.

[2]  Y. Liu,et al.  Electrochemical aptasensor for aflatoxin B1 based on smart host-guest recognition of β-cyclodextrin polymer. , 2019, Biosensors & bioelectronics.

[3]  Zhenzhong Yu,et al.  Paper Electrode-Based Flexible Pressure Sensor for Point-of-Care Immunoassay with Digital Multimeter. , 2019, Analytical chemistry.

[4]  D. Tang,et al.  Wet NH3-Triggered NH2-MIL-125(Ti) Structural Switch for Visible Fluorescence Immunoassay Impregnated on Paper. , 2018, Analytical chemistry.

[5]  Yulin Li,et al.  γ-Fe2O3/La-MOFs@SiO2 for magnetic resonance/fluorescence dual mode imaging and pH-drug delivery , 2018, Materials Letters.

[6]  Zhenzhong Yu,et al.  Metal-Polydopamine Framework: An Innovative Signal-Generation Tag for Colorimetric Immunoassay. , 2018, Analytical chemistry.

[7]  Zhongbin Luo,et al.  Near-Infrared Light-Excited Core-Core-Shell UCNP@Au@CdS Upconversion Nanospheres for Ultrasensitive Photoelectrochemical Enzyme Immunoassay. , 2018, Analytical chemistry.

[8]  C. Zhang,et al.  Controllable synthesis of up-conversion nanoparticles UCNPs@MIL-PEG for pH-responsive drug delivery and potential up-conversion luminescence/magnetic resonance dual-mode imaging , 2018, Journal of Alloys and Compounds.

[9]  Peng Li,et al.  Facile preparation of stable PEG-functionalized quantum dots with glycine-enhanced photoluminescence and their application for screening of aflatoxin B-1 in herbs , 2018 .

[10]  Ki‐Hyun Kim,et al.  Functional hybrid nanostructure materials: Advanced strategies for sensing applications toward volatile organic compounds , 2017 .

[11]  Zhi-Qi Zhang,et al.  An efficient ratiometric fluorescence sensor based on metal-organic frameworks and quantum dots for highly selective detection of 6-mercaptopurine. , 2017, Biosensors & bioelectronics.

[12]  Yang Song,et al.  Single-Site Cobalt Catalysts at New Zr12(μ3-O)8(μ3-OH)8(μ2-OH)6 Metal-Organic Framework Nodes for Highly Active Hydrogenation of Nitroarenes, Nitriles, and Isocyanides. , 2017, Journal of the American Chemical Society.

[13]  C. Nagaraja,et al.  Interpenetrated Metal–Organic Frameworks of Cobalt(II): Structural Diversity, Selective Capture, and Conversion of CO2 , 2017 .

[14]  R. Niessner,et al.  Signal-On Photoelectrochemical Immunoassay for Aflatoxin B1 Based on Enzymatic Product-Etching MnO2 Nanosheets for Dissociation of Carbon Dots. , 2017, Analytical chemistry.

[15]  Sumant Saini,et al.  Nanoporous metal organic frameworks as hybrid polymer-metal composites for drug delivery and biomedical applications. , 2017, Drug discovery today.

[16]  Wenqiang Lai,et al.  Enzyme-controlled dissolution of MnO2 nanoflakes with enzyme cascade amplification for colorimetric immunoassay. , 2017, Biosensors & bioelectronics.

[17]  E. González-Peñas,et al.  An LC-MS/MS method for multi-mycotoxin quantification in cow milk. , 2017, Food chemistry.

[18]  R. Niessner,et al.  Silver Nanolabels-Assisted Ion-Exchange Reaction with CdTe Quantum Dots Mediated Exciton Trapping for Signal-On Photoelectrochemical Immunoassay of Mycotoxins. , 2016, Analytical chemistry.

[19]  Lichun Zhang,et al.  Amino-Functionalized Metal-Organic Frameworks Nanoplates-Based Energy Transfer Probe for Highly Selective Fluorescence Detection of Free Chlorine. , 2016, Analytical chemistry.

[20]  M. Nemati,et al.  Aflatoxin B1 in eggs and chicken livers by dispersive liquid–liquid microextraction and HPLC , 2015, Food additives & contaminants. Part B, Surveillance.

[21]  R. Niessner,et al.  Enzymatic hydrolysate-induced displacement reaction with multifunctional silica beads doped with horseradish peroxidase-thionine conjugate for ultrasensitive electrochemical immunoassay. , 2015, Analytical chemistry.

[22]  K. Tomar,et al.  Stable Multiresponsive Luminescent MOF for Colorimetric Detection of Small Molecules in Selective and Reversible Manner , 2015 .

[23]  Li Wang,et al.  A sensitive fluorescent assay for thiamine based on metal-organic frameworks with intrinsic peroxidase-like activity. , 2015, Analytica chimica acta.

[24]  Chang Ming Li,et al.  Multifunctionalized reduced graphene oxide-doped polypyrrole/pyrrolepropylic acid nanocomposite impedimetric immunosensor to ultra-sensitively detect small molecular aflatoxin B₁. , 2015, Biosensors & bioelectronics.

[25]  S. Saeger,et al.  Novel multiplex fluorescent immunoassays based on quantum dot nanolabels for mycotoxins determination. , 2014, Biosensors & bioelectronics.

[26]  R. Niessner,et al.  Low-cost and highly sensitive immunosensing platform for aflatoxins using one-step competitive displacement reaction mode and portable glucometer-based detection. , 2014, Analytical chemistry.

[27]  D. Drunkler,et al.  Aflatoxin B₁ and M₁ in milk. , 2014, Analytica chimica acta.

[28]  B. Liu,et al.  Two-dimensional metal-organic framework with wide channels and responsive turn-on fluorescence for the chemical sensing of volatile organic compounds. , 2014, Journal of the American Chemical Society.

[29]  Xinwen Guo,et al.  Size- and morphology-controlled NH2-MIL-53(Al) prepared in DMF-water mixed solvents. , 2013, Dalton transactions.

[30]  Yves J. Chabal,et al.  Selective detection of olefins using a luminescent silver-functionalized metal organic framework, RPM3 , 2013 .

[31]  Abhijeet K. Chaudhari,et al.  A Continuous π-Stacked Starfish Array of Two-Dimensional Luminescent MOF for Detection of Nitro Explosives , 2013 .

[32]  Sanjaya D. Perera,et al.  Fabrication of oriented silver-functionalized RPM3 films for the selective detection of olefins. , 2013, Langmuir : the ACS journal of surfaces and colloids.

[33]  S. Rastelli,et al.  Evaluation and Improvement of Extraction Methods for the Analysis of Aflatoxins B1, B2, G1 and G2 from Naturally Contaminated Maize , 2012, Food Analytical Methods.

[34]  J. Long,et al.  Introduction to metal-organic frameworks. , 2012, Chemical reviews.

[35]  Yan Liu,et al.  Global Burden of Aflatoxin-Induced Hepatocellular Carcinoma: A Risk Assessment , 2010, Environmental health perspectives.

[36]  J. Ho,et al.  Disposable electrochemical immunosensor for carcinoembryonic antigen using ferrocene liposomes and MWCNT screen-printed electrode. , 2009, Biosensors & bioelectronics.

[37]  Daniel Gunzelmann,et al.  Synthesis and modification of a functionalized 3D open-framework structure with MIL-53 topology. , 2009, Inorganic chemistry.