Adherence of dermatophyte microconidia and arthroconidia to human keratinocytes in vitro.

The early interaction, adherence, between dermatophyte conidia and human keratinocytes has been studied in vitro. Two spore forms were used: microconidia and arthroconidia produced in vitro. The adherence of spores from three dermatophyte species, Trichophyton rubrum, T interdigitale, and T quinckeanum, was investigated using keratinocyte suspensions from different skin sites. Time-dependent adherence was demonstrated for all fungi studied with maximum adherence occurring between 3 and 4 h. There were no significant differences in adherence rates between the organisms studied. An order of affinity was established between keratinocytes from different sites and significant differences were demonstrated in adherence of microconidia to skin cells derived from sole versus knee. No differences in adherence rates were demonstrated in atopics versus patients with chronic dermatophytosis and normals. Adherence was inhibited, but not abolished, by subinhibitory concentrations of ketoconazole, itraconazole, and griseofulvin. The interaction between microconidia, arthroconidia, and keratinocytes was verified with scanning and transmission electron microscopy.

[1]  J. F. Ryley,et al.  A comparison of phospholipase activity, cellular adherence and pathogenicity of yeasts. , 1985, Journal of general microbiology.

[2]  C. Vroey.  The epidemiology of ringworm (dermatophytosis) , 1985 .

[3]  K. Digre,et al.  Adherence of Candida species to human epidermal corneocytes and buccal mucosal cells: correlation with cutaneous pathogenicity. , 1984, The Journal of investigative dermatology.

[4]  L. J. Douglas,et al.  Relationship between cell surface composition, adherence, and virulence of Candida albicans , 1984, Infection and immunity.

[5]  A. Polak,et al.  Mucormycotic infection in mice following prolonged incubation of spores in vivo and the role of spore agglutinating antibodies on spore germination. , 1984, Sabouraudia.

[6]  S. Klotz,et al.  Adherence and penetration of vascular endothelium by Candida yeasts , 1983, Infection and immunity.

[7]  R. D. King,et al.  Characterization of Candida albicans adherence to human vaginal epithelial cells in vitro , 1983, Infection and immunity.

[8]  C. K. Campbell,et al.  A comparative study of dermatophytosis in coal miners and dermatological outpatients. , 1983, British journal of industrial medicine.

[9]  C. P. Davis,et al.  Modulation of Candida albicans attachment to human epithelial cells by bacteria and carbohydrates , 1983, Infection and immunity.

[10]  L. Samaranayake,et al.  The effect of dietary carbohydrates on the in-vitro adhesion of Candida albicans to epithelial cells. , 1982, Journal of medical microbiology.

[11]  L. Samaranayake,et al.  Factors affecting the in-vitro adherence of the fungal oral pathogen Candida albicans to epithelial cells of human origin. , 1982, Archives of oral biology.

[12]  J. Sobel,et al.  Adherence of Candida albicans to human vaginal and buccal epithelial cells. , 1981, The Journal of infectious diseases.

[13]  R. D. King,et al.  Adherence of Candida albicans and other Candida species to mucosal epithelial cells , 1980, Infection and immunity.

[14]  E. Weigl,et al.  Differentiation of Trichophyton mentagrophytes Arthrospores Controlled by Physical Factors , 1979, Mykosen.

[15]  I. Olsen,et al.  Initiation and aggravation of denture stomatitis by sucrose rinses. , 1976, Scandinavian journal of dental research.

[16]  Z. Hubálek,et al.  Survival of dermatophytes in human skin scales. , 1968, Archives of dermatology.

[17]  F. Fekety,et al.  Application of microtitration techniques to bacteriostatic and bactericidal antibiotic susceptibility testing. , 1968, The Journal of laboratory and clinical medicine.

[18]  S. Rothman,et al.  Defense mechanisms of the skin. , 1963, Annual review of medicine.

[19]  A. Kligman.  The Pathogenesis of Tinea Capitis Due to Microsporum Audouini and Microsporum Canis1 , 1952 .

[20]  A. Kligman.  The pathogenesis of Tinea capitis due to Microsporum audouini and Microsporum canis. I. Gross observations following the inoculation of humans. , 1952, The Journal of investigative dermatology.