The morphological changes in lymphoid organs and peripheral blood indicators in rats after peroral administration of gold nanoparticles

The wide application of nanotechnologies in medicine requires the careful study of various aspects of their potential safety. The effects of prolonged peroral administration of gold nanoparticles on morphological changes in lymphoid organs and indicators of peripheral blood of laboratory animals were investigated in experiment. The gold nanospheres functionalized with thiolated polyethylene glycol sizes 2, 15 and 50 nm were administered orally for 15 days to outbred white rats at a dosage of 190 μg/kg of animal body weight. The standard histological and hematological staining were used for morphological study of lymphoid organs and bone marrow smears. The size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood, especially pronounced after administration of gold nanoparticles with size of 50 nm. The stimulation of myelocytic germ of hematopoiesis was recorded at morphological study of the bone marrow. The signs of strengthening of the processes of differentiation and maturation of cellular elements were found in lymph nodes, which were showed as the increasing number of immunoblasts and large lymphocytes. The quantitative changes of cellular component morphology of lymphoid organs due to activation of migration, proliferation and differentiation of immune cells indicate the presence of immunostimulation effect of gold nanoparticles.

[1]  Valery V Tuchin,et al.  Circulation and distribution of gold nanoparticles and induced alterations of tissue morphology at intravenous particle delivery , 2009, Journal of biophotonics.

[2]  Rebekah Drezek,et al.  In vivo biodistribution of nanoparticles. , 2011, Nanomedicine.

[3]  S. Hsu,et al.  Cytotoxicity and immunological response of gold and silver nanoparticles of different sizes. , 2009, Small.

[4]  G. Frens.  Controlled Nucleation for the Regulation of the Particle Size in Monodisperse Gold Suspensions , 1973 .

[5]  P. Turner,et al.  Administration of substances to laboratory animals: routes of administration and factors to consider. , 2011, Journal of the American Association for Laboratory Animal Science : JAALAS.

[6]  Petra Krystek,et al.  Particle size-dependent organ distribution of gold nanoparticles after intravenous administration. , 2008, Biomaterials.

[7]  Patrice Marche,et al.  Analysis of the toxicity of gold nano particles on the immune system: effect on dendritic cell functions , 2010, Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology.

[8]  Lev A. Dykman,et al.  Gold nanoparticles: preparation, functionalisation and applications in biochemistry and immunochemistry , 2007 .

[9]  Gwo-Bin Lee,et al.  Size-Dependent Attenuation of TLR9 Signaling by Gold Nanoparticles in Macrophages , 2012, The Journal of Immunology.

[10]  Hillyer,et al.  Correlative Instrumental Neutron Activation Analysis, Light Microscopy, Transmission Electron Microscopy, and X-ray Microanalysis for Qualitative and Quantitative Detection of Colloidal Gold Spheres in Biological Specimens , 1998, Microscopy and Microanalysis.

[11]  G. Oberdörster,et al.  Nanotoxicology: An Emerging Discipline Evolving from Studies of Ultrafine Particles , 2005, Environmental health perspectives.

[12]  R. Drezek,et al.  In vivo immune cell distribution of gold nanoparticles in naïve and tumor bearing mice. , 2014, Small.

[13]  Michael B. Cortie,et al.  In Vivo Study of Spherical Gold Nanoparticles: Inflammatory Effects and Distribution in Mice , 2013, PloS one.

[14]  Genesio Pacheco.  Estudos sobre a acção dos colloides metallicos na imunidade , 2022 .

[15]  Scott E McNeil,et al.  Nanotechnology for the biologist , 2005, Journal of leukocyte biology.

[16]  Y. Hung,et al.  Assessment of the In Vivo Toxicity of Gold Nanoparticles , 2009, Nanoscale research letters.

[17]  I. V. Vidyasheva,et al.  Effect of gold nanoparticles on the respiratory activity of peritoneal macrophages , 2009 .

[18]  Peter P. Edwards,et al.  A new hydrosol of gold clusters. 1. Formation and particle size variation , 1993 .

[19]  Pei-Xun Liu,et al.  Toxicologic effects of gold nanoparticles in vivo by different administration routes , 2010, International journal of nanomedicine.

[20]  Xiu Shen,et al.  Size-dependent in vivo toxicity of PEG-coated gold nanoparticles , 2011, International journal of nanomedicine.

[21]  J. Małaczewska.  The in vitro effect of commercially available noble metal nanocolloids on the splenocyte proliferative response and cytokine production in mice. , 2014, Polish journal of veterinary sciences.

[22]  R M Albrecht,et al.  Gastrointestinal persorption and tissue distribution of differently sized colloidal gold nanoparticles. , 2001, Journal of pharmaceutical sciences.

[23]  Hong Liu,et al.  Advances in Cancer Photothermal Therapy , 2010 .

[24]  Dan Peer,et al.  Nanoparticle hydrophobicity dictates immune response. , 2012, Journal of the American Chemical Society.

[25]  R. Drezek,et al.  Gold nanoparticle mediated cancer immunotherapy. , 2014, Nanomedicine : nanotechnology, biology, and medicine.

[26]  Luigi Calzolai,et al.  Gold nanoparticles downregulate interleukin-1β-induced pro-inflammatory responses. , 2013, Small.

[27]  Neus G Bastús,et al.  Peptides conjugated to gold nanoparticles induce macrophage activation. , 2009, Molecular immunology.