Localization of transforming growth factor-beta 1 in mitochondria of murine heart and liver.

Using both electron microscopic immunohistochemistry and cell fractionation techniques, we show that transforming growth factor-beta 1 (TGF-beta 1) is found in mitochondria of rat and mouse cardiac myocytes and rat hepatocytes. Four different polyclonal antibodies, raised against various epitopes encompassing the mature portion of the TGF-beta 1 molecule as well as the pro-region of its precursor, were used for the electron microscopy studies. The localization of TGF-beta 1 in mitochondria was confirmed by detection of the native peptide in mitochondria isolated from rat heart and liver; the majority of native TGF-beta 1 found in liver homogenates was recovered in highly pure mitochondrial fractions. The functional role of TGF-beta in the mitochondrion is unknown at present.

[1]  M. Sporn,et al.  Localization and actions of transforming growth factor-beta s in the embryonic nervous system. , 1991, Development.

[2]  M. Sporn,et al.  Recombinant latent transforming growth factor beta 1 has a longer plasma half-life in rats than active transforming growth factor beta 1, and a different tissue distribution. , 1990, The Journal of clinical investigation.

[3]  R. Schreiber,et al.  Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death , 1990, Nature.

[4]  B. Dahlbäck,et al.  Novel type of very high affinity calcium-binding sites in beta-hydroxyasparagine-containing epidermal growth factor-like domains in vitamin K-dependent protein S. , 1990, The Journal of biological chemistry.

[5]  K. Miyazono,et al.  TGF-β1 binding protein: A component of the large latent complex of TGF-β1 with multiple repeat sequences , 1990, Cell.

[6]  M. Sporn,et al.  Evidence for Differential Regulation of TGFβ1 and TGFβ2 Expression in Vivo by Sandwich Enzyme‐linked Immunosorbent Assays , 1990 .

[7]  M. Sporn,et al.  Expression of transforming growth factor-beta 1 in specific cells and tissues of adult and neonatal mice , 1989, The Journal of cell biology.

[8]  M. Sporn,et al.  Transforming growth factor-beta 1: histochemical localization with antibodies to different epitopes , 1989, The Journal of cell biology.

[9]  Sporn,et al.  Latent transforming growth factor-beta from human platelets. A high molecular weight complex containing precursor sequences. , 1988, The Journal of biological chemistry.

[10]  M. Sporn,et al.  Antibodies to peptide determinants in transforming growth factor beta and their applications. , 1988, Biochemistry.

[11]  M. Sporn,et al.  Role of transforming growth factor-beta in the development of the mouse embryo , 1987, The Journal of cell biology.

[12]  R. Coffey,et al.  Hepatic processing of transforming growth factor beta in the rat. Uptake, metabolism, and biliary excretion. , 1987, The Journal of clinical investigation.

[13]  A. Steiner,et al.  A new technique for removal of amorphous phase tissue water without ice crystal damage: a preparative method for ultrastructural analysis and immunoelectron microscopy. , 1986, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[14]  M. Sporn,et al.  Characterization of a membrane receptor for transforming growth factor-beta in normal rat kidney fibroblasts. , 1984, The Journal of biological chemistry.

[15]  J. Goding.  Use of staphylococcal protein A as an immunological reagent. , 1978, Journal of immunological methods.

[16]  A. Dietz,et al.  Colorimetric determination of serum cholinesterase and its genetic variants by the propionylthiocholine-dithiobis(nitrobenzoic acid)procedure. , 1973, Clinical chemistry.

[17]  W. Dingman,et al.  THE ISOLATION OF CELL NUCLEI FROM RAT BRAIN , 1962, The Journal of cell biology.

[18]  A. Lazarow,et al.  A microspectrophotometric method for the determination of cytochrome oxidase. , 1951, The Journal of biological chemistry.

[19]  M. Sporn,et al.  Antibodies to transforming growth factor-β2 peptides: Specific detection of TGF-β2 in immunoassays , 1990 .

[20]  M. Sporn,et al.  Sandwich Enzyme-Linked Immunosorbent Assays (Selisas) Quantitate and Distinguish Two Forms of Transforming Growth Factor-Beta (TGF-β1 and TGF-β2) in Complex Biological Fluids , 1989 .

[21]  M. Sporn,et al.  Transforming growth factor beta-1 in acute myocardial infarction in rats. , 1988, Growth factors.

[22]  T. Phillips,et al.  Liquid nitrogen‐based quick freezing: Experiences with bounce‐free delivery of cholinergic nerve terminals to a metal surface , 1984 .

[23]  L. Mela,et al.  Isolation of mitochondria with emphasis on heart mitochondria from small amounts of tissue. , 1979, Methods in enzymology.

[24]  A. Spurr.  A low-viscosity epoxy resin embedding medium for electron microscopy. , 1969, Journal of ultrastructure research.

[25]  H. Lardy,et al.  [15] Isolation of liver or kidney mitochondria , 1967 .