Toward the mapping of physiological and agronomic characters on a rice function map: QTL analysis and comparison between QTLs and expressed sequence tags

Abstract We have constructed a rice function map by collating the results on quantitative trait loci (QTLs) for 23 important physiological and agronomic characters (including 13 newly measured traits) obtained using backcross inbred lines of japonica Nipponbare×indica Kasalath. Using these materials, The Rice Genome project (RGP) has developed a high-density genetic map. QTLs controlling yield did not overlap with those controlling the morphological and physiological traits supposed to relate to yield, such as photosynthetic ability. This result suggests that these traits do not influence yield, at least in this genetic background and environment. QTLs controlling yield also did not overlap with the structural genes controlling carbon metabolism (rbcS, cytosolic or plastidic fructose-1,6-bisphosphate, R-enzyme, and sucrose synthase).The combination of a function map and results from the RGP can be advantageous. The utility of this map is discussed.

[1]  D. Arnon.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. , 1949, Plant physiology.

[2]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[3]  P. Rundel.  Stable Isotopes in Ecological Research , 1989, Ecological Studies.

[4]  P. J. Davies,et al.  Evidence from Polygene Mapping for a Causal Relationship between Potato Tuber Dormancy and Abscisic Acid Content , 1997, Plant physiology.

[5]  S. Lin,et al.  A 300 kilobase interval genetic map of rice including 883 expressed sequences , 1994, Nature Genetics.

[6]  S. Briggs,et al.  Plant genomics: more than food for thought. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[7]  A. Makino,et al.  Colorimetric Measurement of Protein Stained with Coomassie Brilliant Blue R on Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis by Eluting with Formamide , 1986 .

[8]  G. C. Yencho,et al.  Quantitative trait loci for polyamine content in an RFLP-mapped potato population and their relationship to tuberization , 1999 .

[9]  S. Tanksley.  Mapping polygenes. , 1993, Annual review of genetics.

[10]  A. Peeters,et al.  Genetic approaches in plant physiology , 1997 .

[11]  S. Lin,et al.  A high-density rice genetic linkage map with 2275 markers using a single F2 population. , 1998, Genetics.

[12]  S. Tsunoda,et al.  Curvilinear Regression of the Leaf Photosynthetic Rate on Leaf Nitrogen Content among Strains of Oryza Species , 1971 .

[13]  角田 重三郎,et al.  Curvilinear Regression of the Leaf Photosynthetic Rate on Leaf Nitrogen Content among Strains of Oryza Species , 1971 .

[14]  A. Osada,et al.  Studies on the Relationship between Photosynthesis and Varietal Adaptability for Heavy Manuring : 3. Effects of photosynthetic characteristics on dry-matter-production and ripening of rice varieties. , 1965 .

[15]  D. Robertson.  A possible technique for isolating genic DNA for quantitative traits in plants , 1985 .

[16]  K. Zheng,et al.  Analysis of QTL×environment interaction for yield components and plant height in rice , 1997, Theoretical and Applied Genetics.

[17]  Agricultural genomics comes of age , 1998, Nature Biotechnology.

[18]  L. T. Evans,et al.  Some physiological aspects of the domestication and improvement of rice (Oryza spp.) , 1983 .

[19]  T. Sasaki,et al.  The rice genome project in Japan. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[20]  J. Prioul,et al.  Dissecting complex physiological functions through the use of molecular quantitative genetics , 1997 .

[21]  S. Lin,et al.  Mapping quantitative trait loci controlling seed dormancy and heading date in rice, Oryza sativa L., using backcross inbred lines , 1998, Theoretical and Applied Genetics.