Immunological characterization of mononuclear cells in peripheral blood and regional lymph nodes of breast cancer patients

Mononuclear cells from peripheral blood and draining lymph nodes of 40 patients with invasive locoregional breast cancer were examined for immunological cell surface markers (E, EAhuman, EAox, EAC, SIg pos.). Concomitantly, blood lymphocytes from 36 healthy women and axillary and mesenteric lymph‐nodes from patients without malignant diseases were tested as controls. In peripheral blood of tumor patients E rosette‐forming cells were slightly diminished as compared to the control group, whereas EAox and EAC rosetteforming cells were increased. These differences may be age‐dependent rather than tumor‐related. In the draining lymph nodes of breast cancer patients as well as in the control lymph nodes, the percentages of EAC rosette‐forming cells and SIg positive lymphocytes were significantly increased compared to peripheral blood, whereas E and EAhuman rosette values remained unchanged. Percentages of EAox rosettes on the other hand were strongly diminished in the draining lymph nodes, suggesting that the EAhuman and EAox rosetting techniques detect 2 types of Fc‐receptor bearing cells. No significant differences were found between the cell surface marker analysis of tumor‐free and metastatic lymph nodes of breast cancer patients and the control lymph nodes.

[1]  S. Argov,et al.  Natural cytotoxicity in man: activity of lymph node and tumor‐infiltrating lymphocytes , 1977, European journal of immunology.

[2]  J. Cesarini,et al.  Human peripheral Null lymphocytes I. Isolation, immunological and functional characterization , 1977, European journal of immunology.

[3]  R. Herberman,et al.  A functional comparison of human Fc-receptor-bearing lymphocytes active in natural cytotoxicity and antibody-dependent cellular cytotoxicity. , 1977, Journal of immunology.

[4]  E. Klein,et al.  Characterization of human lymphocyte subpopulations for cytotoxicity against tumor‐derived monolayer cultures , 1977, International journal of cancer.

[5]  A. Desplaces,et al.  Immunological study of axillary lymphnodes in patients with breast cancer. , 1977, European journal of cancer.

[6]  R. Whitehead,et al.  T AND B LYMPHOCYTES IN BREAST CANCER , 1977, The Lancet.

[7]  C. Naspitz,et al.  Cell‐mediated immunity in patients with carcinoma. Correlation between clinical stage and immunocompetence , 1976, Cancer.

[8]  H. Ioachim,et al.  Decreased T-lymphocytes in patients with mammary cancer. , 1976, American journal of clinical pathology.

[9]  J. Revillard,et al.  Distribution of Cells Binding Erythrocyte‐Antibody (EA) Complexes in Human Lymphoid Populations , 1976, Scandinavian journal of immunology.

[10]  C. Alexopoulos,et al.  AGE DEPENDENCE OF T LYMPHOCYTES , 1976, The Lancet.

[11]  F. Cockburn,et al.  BUFFER THERAPY AND INTRAVENTRICULAR HÆMORRHAGE , 1976, The Lancet.

[12]  H. Pross,et al.  Cytotoxic lymphocytes from normal donors. A functional marker of human non-T lymphocytes. , 1975, Clinical and experimental immunology.

[13]  W. Fridman,et al.  Cell-mediate cytotoxicity in vitro of human lymphocytes against a tissue culture melanoma cell line (igr3). , 1975, Journal of immunology.

[14]  R. Williams,et al.  AGE-RELATED CHANGES IN T AND B CELLS , 1975, The Lancet.

[15]  G. Wernick,et al.  Immunologic competence of regional lymph nodes in patients with breast cancer , 1975, Cancer.

[16]  R. Good,et al.  In vitro studies of axillary lymph node cells in patients with breast cancer. , 1975, Journal of the National Cancer Institute.

[17]  C. Potvin,et al.  Thymus-derived lymphocytes in patients with solid malignancies. , 1975, Clinical immunology and immunopathology.

[18]  M. Blacow,et al.  INDUCTION OF LABOUR , 1975, The Lancet.

[19]  H. Grey,et al.  Identification, Enumeration, and Isolation of B and T Lymphocytes from Human Peripheral Blood , 1974, Scandinavian journal of immunology.

[20]  T. Han,et al.  Cell-mediated immune status of breast cancer patients: evaluation by skin tests, lymphocyte stimulation, and counts of rosette-forming cells. , 1974, Journal of the National Cancer Institute.

[21]  G. Brittinger,et al.  DECREASE OF T LYMPHOCYTES DURING AGEING , 1974 .

[22]  E. Carosella,et al.  Rosette-forming T cells in human peripheral blood at different ages. , 1974, Cellular immunology.

[23]  J. Kalden,et al.  Letter: Sézary syndrome. , 1974, Lancet.

[24]  H. Wigzell,et al.  Human Lymphocyte Subpopulations: Classification According to Surface Markers and/or Functional Characteristics , 1973, Transplantation reviews.

[25]  H. Wigzell,et al.  Lymphocyte Mediated Cytotoxicity in Vitro. Induction and Inhibition by Humoral Antibody and Nature of Effector Cells , 1972, Transplantation reviews.

[26]  I. Maclennan.  Antibody in the Induction and Inhibition of Lymphocyte Cytotoxicity , 1972, Transplantation reviews.

[27]  R. Leber,et al.  The Dubious Case for Conservative Operation in Operable Cancer of the Breast , 1972, Annals of surgery.

[28]  I. W. Wallace,et al.  Axillary nodes in breast cancer. , 1972, Lancet.

[29]  H. Bloom,et al.  Impact of tumor grade and host resistance on survival of women with breast cancer , 1971, Cancer.

[30]  J. R. Amerson,et al.  Immunologic Competence of Regional Lymph Nodes in Patients with Mammary Cancer , 1971, Annals of surgery.

[31]  V. Nussenzweig,et al.  A POPULATION OF LYMPHOCYTES BEARING A MEMBRANE RECEPTOR FOR ANTIGEN-ANTIBODY-COMPLEMENT COMPLEXES , 1970, The Journal of experimental medicine.

[32]  H. Bloom,et al.  Host Resistance and Survival in Carcinoma of Breast: A Study of 104 Cases of Medullary Carcinoma in a Series of 1,411 Cases of Breast Cancer Followed for 20 years* , 1970, British medical journal.

[33]  W. Hammond,et al.  Retained regional lymph nodes: Effect on metastases and recurrence after tumor removal , 1970, Cancer.

[34]  G. Crile,et al.  Possible role of uninvolved regional nodes in preventing metastasis from breast cancer , 1969, Cancer.

[35]  W. Hammond,et al.  The role of the regional lymph nodes in the immunity to a chemically induced sarcoma in C3H mice. , 1969, Cancer research.

[36]  G. Crile.  Results of Simple Mastectomy without Irradiation in the Treatment of Operative Stage I Cancer of the Breast , 1968, Annals of surgery.

[37]  A. Saxon,et al.  Human lymph node lymphocytes fail to effect lysis of antibody-coated target cells. , 1977, Clinical and experimental immunology.

[38]  O. Eremin,et al.  T and B lymphocyte populations in human normal lymph node, regional tumour lymph node and inflammatory lymph node. , 1976, International archives of allergy and applied immunology.

[39]  S. Frøland,et al.  Human Lymphocytes with Receptors for IgG , 1974 .

[40]  A. Stuart,et al.  Letter: T and B cells in human spleen. , 1974, Lancet.

[41]  T. Hallberg,et al.  Opsonic adherence of sensitized ox red cells to human lymphocytes as measured by rosette formation. , 1973, International archives of allergy and applied immunology.

[42]  K. Ramming,et al.  The role of the regional lymph nodes in the development of host immunological response to tumors. , 1971, The American journal of roentgenology, radium therapy, and nuclear medicine.

[43]  J. Stjernswärd,et al.  Tumor-distinctive cellular immunity to human sarcoma and carcinoma. , 1971, Israel journal of medical sciences.

[44]  G. Crile.  The effect on metastasis of removing or irradiating regional nodes of mice. , 1968, Surgery, gynecology & obstetrics.

[45]  P. Astrup,et al.  Oxygen affinity and acid-base status of human blood during exposure to hypoxia and carbon monoxide. , 1968, Scandinavian journal of clinical and laboratory investigation. Supplementum.

[46]  A. Böyum,et al.  Isolation of leucocytes from human blood. A two-phase system for removal of red cells with methylcellulose as erythrocyte-aggregating agent. , 1968, Scandinavian journal of clinical and laboratory investigation. Supplementum.