Rapid and high-efficient generation of mutant mice using freeze-thawed embryos of the C57BL/6J strain

[1]  Hideki Uosaki,et al.  Tbx6 Induces Nascent Mesoderm from Pluripotent Stem Cells and Temporally Controls Cardiac versus Somite Lineage Diversification. , 2018, Cell stem cell.

[2]  Takashi Yamamoto,et al.  Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation , 2018, Experimental animals.

[3]  K. C. K. Lloyd,et al.  Efficient mouse genome engineering by CRISPR-EZ technology , 2018, Nature Protocols.

[4]  B. Schermer,et al.  An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes , 2018, bioRxiv.

[5]  Y. Yamashita,et al.  Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse. , 2016, Developmental biology.

[6]  Takashi Yamamoto,et al.  Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice , 2016, Biology Open.

[7]  J. Swinnen,et al.  CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing , 2016, Scientific Reports.

[8]  T. Takeo,et al.  N-acetyl cysteine prolonged the developmental ability of mouse two-cell embryos against oxidative stress at refrigerated temperatures. , 2016, Cryobiology.

[9]  Lin He,et al.  Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes* , 2016, The Journal of Biological Chemistry.

[10]  T. Takemoto,et al.  Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing , 2015, Scientific Reports.

[11]  Wei Yan,et al.  Pervasive Genotypic Mosaicism in Founder Mice Derived from Genome Editing through Pronuclear Injection , 2015, PloS one.

[12]  M. Ohmuraya,et al.  Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes , 2015, BMC Biotechnology.

[13]  Ankit Malhotra,et al.  Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease , 2015, Genetics.

[14]  Hidemasa Bono,et al.  CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites , 2014, Bioinform..

[15]  J. Doudna,et al.  The new frontier of genome engineering with CRISPR-Cas9 , 2014, Science.

[16]  Takashi Yamamoto,et al.  Simple knockout by electroporation of engineered endonucleases into intact rat embryos , 2014, Scientific Reports.

[17]  Min Zhang,et al.  Somatic mosaicism and allele complexity induced by CRISPR/Cas9 RNA injections in mouse zygotes. , 2014, Developmental biology.

[18]  Daesik Kim,et al.  Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins , 2014, Genome research.

[19]  Satoru Takahashi,et al.  Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system , 2014, Mammalian Genome.

[20]  Yoshitaka Fujihara,et al.  Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA , 2013, Scientific Reports.

[21]  C. Barbas,et al.  ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. , 2013, Trends in biotechnology.

[22]  James E. DiCarlo,et al.  RNA-Guided Human Genome Engineering via Cas9 , 2013, Science.

[23]  Le Cong,et al.  Multiplex Genome Engineering Using CRISPR/Cas Systems , 2013, Science.

[24]  Yongyan Wu,et al.  Efficient Delivery of DNA and Morpholinos into Mouse Preimplantation Embryos by Electroporation , 2012, PloS one.

[25]  J. Doudna,et al.  A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity , 2012, Science.

[26]  T. Kondo,et al.  Short-term storage and transport at cold temperatures of 2-cell mouse embryos produced by cryopreserved sperm. , 2010, Journal of the American Association for Laboratory Animal Science : JAALAS.

[27]  N. Nakagata,et al.  Use of frozen-thawed oocytes for efficient production of normal offspring from cryopreserved mouse spermatozoa showing low fertility. , 2005, Comparative medicine.

[28]  R. Jaenisch,et al.  Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[29]  N. Nakagata.  High survival rate of unfertilized mouse oocytes after vitrification. , 1989, Journal of reproduction and fertility.

[30]  Y. Matsuda,et al.  Chromosomal analysis in mouse eggs fertilized in vitro with sperm exposed to ultraviolet light (UV) and methyl and ethyl methanesulfonate (MMS and EMS). , 1988, Mutation research.

[31]  H. Nishizono,et al.  Cleavage Speed and Blastomere Number in DBA/2J Compared with C57BL/6J Mouse Embryos. , 2017, Journal of the American Association for Laboratory Animal Science : JAALAS.