HLA-A2.1-associated peptides from a mutant cell line: a second pathway of antigen presentation.

Peptides extracted from HLA-A2.1 class I major histocompatibility complex (MHC) molecules expressed on the antigen processing mutant CEMx721.174.T2 were characterized by electrospray ionization-tandem mass spectrometry. Only seven dominant peptides were found, in contrast to over 200 associated with HLA-A2.1 on normal cells. These peptides were derived from the signal peptide domains of normal cellular proteins, were usually larger than nine residues, and were also associated with HLA-A2.1 in normal cells. These results suggest that proteolysis of signal peptide domains in the endoplasmic reticulum is a second mechanism for processing and presentation of peptides for association with class I molecules.

[1]  T. Braciale,et al.  Differences in antigen presentation to MHC class I-and class II- restricted influenza virus-specific cytolytic T lymphocyte clones , 1986, The Journal of experimental medicine.

[2]  J R Yates,et al.  Protein sequencing by tandem mass spectrometry. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[3]  R. Feinman,et al.  Molecular and biochemical characterization of a novel gamma-interferon-inducible protein. , 1988, The Journal of biological chemistry.

[4]  M. Bevan,et al.  Introduction of soluble protein into the class I pathway of antigen processing and presentation , 1988, Cell.

[5]  A. Townsend,et al.  Antigen recognition by class I-restricted T lymphocytes. , 1989, Annual review of immunology.

[6]  H. Ljunggren,et al.  Association of class I major histocompatibility heavy and light chains induced by viral peptides , 1989, Nature.

[7]  P. Cresswell,et al.  Presentation of viral antigen controlled by a gene in the major histocompatibility complex , 1990, Nature.

[8]  Hidde L. Ploegh,et al.  Direct binding of peptide to empty MHC class I molecules on intact cells and in vitro , 1990, Cell.

[9]  H. Rammensee,et al.  Identification of classical minor histocompatibility antigen as cell-derived peptide , 1990, Nature.

[10]  H. Rammensee,et al.  Characterization of naturally occurring minor histocompatibility peptides including H-4 and H-Y. , 1990, Science.

[11]  Hans-Georg Rammensee,et al.  Isolation and analysis of naturally processed viral peptides as recognized by cytotoxic T cells , 1990, Nature.

[12]  R. Demars,et al.  A gene in the human major histocompatibility complex class II region controlling the class I antigen presentation pathway , 1990, Nature.

[13]  T. Elliott,et al.  Assembly of MHC class I molecules analyzed in vitro , 1990, Cell.

[14]  Hidde L. Ploegh,et al.  Empty MHC class I molecules come out in the cold , 1990, Nature.

[15]  H. Grey,et al.  The minimal number of class II MHC-antigen complexes needed for T cell activation. , 1990, Science.

[16]  S. Nathenson,et al.  Isolation of an endogenously processed immunodominant viral peptide from the class I H–2Kb molecule , 1990, Nature.

[17]  Itoh,et al.  Molecular cloning, expression, and chromosome 19 localization of a human Ro/SS-A autoantigen. , 1990, The Journal of clinical investigation.

[18]  H. Rammensee,et al.  Identification of naturally processed viral nonapeptides allows their quantification in infected cells and suggests an allele-specific T cell epitope forecast , 1991, The Journal of experimental medicine.

[19]  O. Rötzschke,et al.  Naturally-occurring peptide antigens derived from the MHC class-I-restricted processing pathway. , 1991, Immunology today.

[20]  D. R. Madden,et al.  The structure of HLA-B27 reveals nonamer self-peptides bound in an extended conformation , 1991, Nature.

[21]  V. Engelhard,et al.  Influenza A-specific, HLA-A2.1-restricted cytotoxic T lymphocytes from HLA-A2.1 transgenic mice recognize fragments of the M1 protein. , 1991, Journal of immunology.

[22]  Hidde L. Ploegh,et al.  Peptide selection by MHC class I molecules , 1991, Nature.

[23]  Timothy E. Elliott,et al.  The binding affinity and dissociation rates of peptides for class I major histocompatibility complex molecules , 1991, European journal of immunology.

[24]  D. R. Madden,et al.  Identification of self peptides bound to purified HLA-B27 , 1991, Nature.

[25]  Robert DeMars,et al.  Restored expression of major histocompatibility class I molecules by gene transfer of a putative peptide transporter , 1991, Nature.

[26]  M. Luscher,et al.  Peptide binding to class I MHC on living cells and quantitation of complexes required for CTL lysis , 1991, Nature.

[27]  H. Rammensee,et al.  Allele-specific motifs revealed by sequencing of self-peptides eluted from MHC molecules , 1991, Nature.