The Genetic Basis of High Resistance to Rice Yellow Mottle Virus (RYMV) in Cultivars of Two Cultivated Rice Species.

Three cultivars of Oryza sativa (IR64, Azucena, and Gigante) and four cultivars of O. glaberrima (Tog5681, Tog5673, CG14, and SG329) were evaluated for their resistance to two isolates of rice yellow mottle virus (RYMV) by enzyme-linked immunosorbent assay (ELISA) and symptomatology. Cultivars Tog5681 and Gigante were highly resistant, and no symptoms were observed when either virus isolate was inoculated at 10 or 20 days postgermination and assayed by ELISA at 7, 14, 22, 35, 50, or 64 days postinoculation. Azucena showed a partial resistance, whereas the other cultivars were susceptible. Symptom appearance was associated with increase in ELISA absorbance in the systemically infected leaves. The best discrimination among the cultivars occurred when the plants were inoculated at 10 days postgermination. Crosses were made between the highly resistant (Gigante and Tog5681) and the susceptible (IR64) cultivars to determine the genetic basis of resistance to RYMV. Evaluation of F1 hybrids and interspecific progenies, as well as the segregation of resistance in F2 and F3 lines of the IR64 × Gigante cross, provided results consistent with the presence of a single recessive resistance gene common to Tog5681 and Gigante.

[1]  N. Ahmadi,et al.  Genetic basis and mapping of the resistance to rice yellow mottle virus. I. QTLs identification and relationship between resistance and plant morphology , 1998, Theoretical and Applied Genetics.

[2]  N. Ahmadi,et al.  Genetic basis and mapping of the resistance to rice yellow mottle virus. II. Evidence of a complementary epistasis between two QTLs , 1998, Theoretical and Applied Genetics.

[3]  M. Yeager,et al.  Movement of rice yellow mottle virus between xylem cells through pit membranes. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[4]  M. Abo,et al.  Rice yellow mottle virus (RYMV) in Africa: evolution, distribution, economic significance on sustainable rice production and management strategies. , 1997 .

[5]  N. Ahmadi,et al.  A major quantitative trait locus for rice yellow mottle virus resistance maps to a cluster of blast resistance genes on chromosome 12. , 1997, Phytopathology.

[6]  S. Mccouch,et al.  Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.) , 1997, Theoretical and Applied Genetics.

[7]  C. Fauquet,et al.  Nucleotide sequence and genome characterization of rice yellow mottle virus RNA. , 1994, The Journal of general virology.

[8]  G. Thottappilly.  Plant virus diseases of importance to African agriculture , 1992 .

[9]  R. Michelmore,et al.  Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[10]  A. N. Adams,et al.  Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. , 1977, The Journal of general virology.

[11]  O. Traoré,et al.  Characterization of rice yellow mottle virus isolatesin Sudano-Sahelian areas , 1997, Archives of Virology.

[12]  C. P. Paul,et al.  Diallel analysis of resistance to rice yellow mottle virus in African rice Oryza glaberrima Steud. , 1995 .

[13]  G. Thottappilly,et al.  Evaluation of resistance to rice yellow mottle virus in Oryza species. , 1993 .

[14]  R. Hull.  The Sobemovirus Group , 1988 .

[15]  C. W. Kuhn,et al.  Resistance responses in cowpea to southern bean mosaic virus based on virus accumulation and symptomatology , 1986 .

[16]  E. M. Ball,et al.  Factors influencing virus titer in maize dwarf mosaic virus-infected sorghum. , 1985 .

[17]  C. Fatokun,et al.  Reaction of Oryza glaberrina accessions to rice yellow mottle virus. , 1983 .

[18]  C. Fauquet,et al.  Isolation of the rice yellow mottle virus in Ivory Coast. , 1977 .