Evaluation of vascular effects after photodynamic and photothermal therapies using benzoporphyrin derivative monoacid ring A on a rodent dorsal skinfold model

Background and Objectives: Pulsed dye laser (PDL) irradiation is the standard clinical treatment for vascular lesions. However, PDL treatment of port wine stain birthmarks (PWS) is variable and unpredictable. Photodynamic therapy (PDT) using benzoporphyrin derivative monoacid ring A (BPD) and yellow light may induce substantial vascular effects and potentially offer a more effective treatment. In this study, we utilize a rodent dorsal skinfold model to evaluate the vascular effects of BPD-PDT at 576 nm as compared to PDL. Study Design/Materials and Methods: A dorsal skinfold window was created on the backs of female Sprague-Dawley rats, allowing epidermal and subdermal irradiation and subdermal imaging. One mg/kg BPD was administered intravenously via a jugular venous catheter. Study groups were: control (no BPD, no light), PDL (585 nm, τp 1.5 ms, 10 J/cm2), and PDT (BPD + continuous wave irradiation (CW) at 576nm, τp 16 min, 96 J/cm2). Vessels were imaged and assessed for damage using laser speckle imaging (LSI) before, immediately after, and 18 hours post-intervention. Results: Epidermal irradiation was accomplished without blistering, scabbing or ulceration. PDL and PDT resulted in similar reductions in vascular perfusion 18 hours post-intervention (34.6% and 33.4%, respectively). Conclusions: BPD-PDT can achieve safe and selective vascular effects and may offer an alternative therapeutic option for treatment of hypervascular skin lesions including PWS birthmarks.

[1]  Bernard Choi,et al.  Laser speckle imaging for monitoring blood flow dynamics in the in vivo rodent dorsal skin fold model. , 2004, Microvascular research.

[2]  Sol Kimel,et al.  Combined photodynamic and photothermal induced injury enhances damage to in vivo model blood vessels , 2004, Lasers in Surgery and Medicine.

[3]  B. Majaron,et al.  Computational model to evaluate port wine stain depth profiling using pulsed photothermal radiometry. , 2004, Journal of biomedical optics.

[4]  J. Houle,et al.  DURATION OF SKIN PHOTOSENSITIVITY AND INCIDENCE OF PHOTOSENSITIVITY REACTIONS AFTER ADMINISTRATION OF VERTEPORFIN , 2002, Retina.

[5]  A. Ros,et al.  Flashlamp pulsed dye laser and argon‐pumped dye laser in the treatment of port‐wine stains: a clinical and histological comparison , 2002, The British journal of dermatology.

[6]  S. Marcus.  Lasers in Photodynamic Therapy , 2001 .

[7]  J. Briers,et al.  Laser Doppler, speckle and related techniques for blood perfusion mapping and imaging. , 2001, Physiological measurement.

[8]  A J Welch,et al.  Use of osmotically active agents to alter optical properties of tissue: Effects on the detected fluorescence signal measured through skin , 2001, Lasers in surgery and medicine.

[9]  M. Moskowitz,et al.  Dynamic Imaging of Cerebral Blood Flow Using Laser Speckle , 2001, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[10]  A. Kauvar,et al.  High-fluence modified pulsed dye laser photocoagulation with dynamic cooling of port-wine stains in infancy. , 2000, Archives of dermatology.

[11]  N. Bressler,et al.  A preliminary study of photodynamic therapy using verteporfin for choroidal neovascularization in pathologic myopia, ocular histoplasmosis syndrome, angioid streaks, and idiopathic causes. , 2000, Archives of ophthalmology.

[12]  S. González,et al.  Wavelength and fluence effect on vascular damage with photodynamic therapy on skin. , 2000, The Journal of investigative dermatology.

[13]  Gracie Vargas,et al.  Laser Fluence for Permanent Damage of Cutaneous Blood Vessels , 1999, Photochemistry and photobiology.

[14]  Peter K. Kik,et al.  Analysis of acute vascular damage after photodynamic therapy using benzoporphyrin derivative (BPD) , 1999, British Journal of Cancer.

[15]  G. Lin,et al.  Skin Necrosis due to Photodynamic Action of Benzoporphyrin Depends on Circulating Rather than Tissue Drug Levels: Implications for Control of Photodynamic Therapy , 1998, Photochemistry and photobiology.

[16]  J. Nelson,et al.  Photodynamic therapy of actinic keratosis with topical 5-aminolevulinic acid. A pilot dose-ranging study. , 1997, Archives of dermatology.

[17]  R. Anderson,et al.  Predictive dosimetry for threshold phototoxicity in photodynamic therapy on normal skin: red wavelengths produce more extensive damage than blue at equal threshold doses. , 1997, The Journal of investigative dermatology.

[18]  K. Arndt,et al.  Lasers in cutaneous and aesthetic surgery , 1997 .

[19]  Michael W. Berns,et al.  Epidermal melanin absorption in human skin , 1996, European Conference on Biomedical Optics.

[20]  A.J. Welch,et al.  Measurements of argon laser light attenuation in the skin 'in vivo' using a unique animal model , 1989, Images of the Twenty-First Century. Proceedings of the Annual International Engineering in Medicine and Biology Society,.