Mode of action studies of the acaricidal agent, epingaione

Summary Activity directed isolation studies of the ethanol extract of Bontia daphnoides L (Myoporaceae) leaves and stems led to the isolation of epingaione, a sesquiterpene furan. This compound was previously described by Chinnock et al. (1987) from the same plant. The compound showed growth regulatory activities on gravid adult female Boophilus microplus Canestrini (the southern cattle tick). The dose required for inhibiting the hatching of B. microplus eggs by 50% (Fid50)was 0.4±0.06 mg/g of the tick body weight. The Fid50 dose inhibited the sequestration of protein into eggs oviposited by 80%. These eggs were non-agglutinated and wrinkled. Histological examination of ovarian sections from the treated ticks revealed significant degeneration of funicle cells and reduction in yolk content.

[1]  A. Mansingh,et al.  Pesticidal Potential of Tropical Plants—II. Acaricidal Activity of Crude Extracts of Several Jamaican Plants , 1998 .

[2]  L. Williams.  Adverse effects of extracts of Artocarpus altilis Park, and Azadirachta indica (A. Juss) on the reproductive physiology of the adult female tick, Boophilus microplus (Canest.) , 1993 .

[3]  H. Wilps,et al.  The effects of neem oil and azadirachtin on mortality, flight activity, and energy metabolism of Schistocerca gregaria forskal—A comparison between laboratory and field locusts , 1992 .

[4]  L. Williams,et al.  Acaricidal and insecticidal activities of three synthetic oxazolidinones , 1991 .

[5]  E. Ghisalberti,et al.  Epingaione from Bontia daphnoides , 1987 .

[6]  L. Brattsten.  Cytochrome P-450 Involvement in the Interactions Between Plant Terpenes and Insect Herbivores , 1983 .

[7]  H. Rembold,et al.  The effects of azadirachtin on the endocrine control of moulting in Locusta migratoria , 1983 .

[8]  S. Rawlins,et al.  Inhibition of oviposition in the cattle tick Boophilus microplus by certain acaricides , 1979 .

[9]  Fumio Matsumura,et al.  Toxicology of Insecticides , 1975, Springer US.

[10]  Sutherland,et al.  Terpenoid chemistry. XXI. (–)-Epingaione, (–)-Dehydrongaione, (–)-Dehydroepingaione, and (–)-Deisopropylngaione, toxic furanoid sesquiterpenoid ketones from Myoporum deserti , 1973 .

[11]  J. Busvine.  A critical review of the techniques for testing insecticides , 1971 .

[12]  R. O'brien.  Mode of action of insecticides. , 1966, Annual review of entomology.

[13]  Ennis Layne,et al.  SPECTROPHOTOMETRIC AND TURBIDIMETRIC METHODS FOR MEASURING PROTEINS , 1957 .