Synthesis and antibacterial activity of colloidal silver prepared by electrochemical method

Abstract Study was conducted on the electrochemical method of preparing colloidal silver at low DC voltage. A natural stabilizer was used as a carob bean gum from the carob bean. Using a UV-Vis spectrophotometer and a transmission electron microscope (TEM), colloidal silver (silver nanoparticles) was characterized. It is possible to produce colloidal silver of satisfactory quality by means of this electrochemical method. Silver nanoparticles have exhibited surface plasmon resonance as determined by UV-vis analysis. Based on the TEM analysis, the silver nanoparticles appear to be spherical except in sample E1, where they appear rod-shaped and micrometer-sized. Other three samples had particle sizes ranging from 23–52 nm. In a qualitative analysis of colloidal silver solutions, two types of antibacterial activity were observed. At all dilutions, some colloidal solutions showed better antibacterial activity against gram-positive bacteria (S. aureus, S. pyogenes, E. faecalis) while others displayed better antibacterial activity against gram-negative bacteria (S. enteridis, P. aeruginosa, E. coli).

[1]  Jianbo Xiao,et al.  Nanotechnologies in Food Science: Applications, Recent Trends, and Future Perspectives , 2020, Nano-micro letters.

[2]  Damir Barbir,et al.  The use of PWHM and Mie methods in estimation of colloidal silver particle size obtained by chemical precipitation with sodium borohydride , 2019, Chemical Industry.

[3]  R. Duval,et al.  Limitations of Recent Studies Dealing with the Antibacterial Properties of Silver Nanoparticles: Fact and Opinion , 2019, Nanomaterials.

[4]  J. Rodriguez-Reyes,et al.  Synthesis of Highly Concentrated Suspensions of Silver Nanoparticles by Two Versions of the Chemical Reduction Method , 2018, Methods and protocols.

[5]  Manisha Pandey,et al.  An overview of application of silver nanoparticles for biomaterials in dentistry. , 2018, Materials science & engineering. C, Materials for biological applications.

[6]  F. Barras,et al.  Silver and Antibiotic, New Facts to an Old Story , 2018, Antibiotics.

[7]  S. Gurunathan,et al.  Antibacterial Efficacy of Silver Nanoparticles on Endometritis Caused by Prevotella melaninogenica and Arcanobacterum pyogenes in Dairy Cattle , 2018, International journal of molecular sciences.

[8]  Smita S. Kumar,et al.  An enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach , 2018, Environmental Science and Pollution Research.

[9]  Prateek Shrivastava,et al.  World health organization releases global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics , 2018 .

[10]  S. Muthupandian,et al.  Antibacterial efficacy of silver nanoparticles against multi-drug resistant clinical isolates from post-surgical wound infections. , 2017, Microbial pathogenesis.

[11]  Khalid Saeed,et al.  Nanoparticles: Properties, applications and toxicities , 2017, Arabian Journal of Chemistry.

[12]  A. Hamood,et al.  The ability of a colloidal silver gel wound dressing to kill bacteria in vitro and in vivo. , 2017, Journal of wound care.

[13]  M. D. Torres,et al.  Novel designed VmCT1 analogs with increased antimicrobial activity. , 2017, European journal of medicinal chemistry.

[14]  Stephan Harbarth,et al.  Will 10 Million People Die a Year due to Antimicrobial Resistance by 2050? , 2016, PLoS medicine.

[15]  Prepared Standard methods for the examination of water and wastewater , 2016 .

[16]  S. Ghasemi,et al.  Silver nanoparticles prepared in presence of ascorbic acid and gelatin, and their electrocatalytic application , 2014, Bulletin of Materials Science.

[17]  S. Gaisford,et al.  An in vitro test of the efficacy of silver-containing wound dressings against Staphylococcus aureus and Pseudomonas aeruginosa in simulated wound fluid. , 2014, International journal of pharmaceutics.

[18]  Ji-Min Wu,et al.  Applications of nanotechnology in biomedicine , 2013 .

[19]  Yogesha,et al.  An optical tweezer-based study of antimicrobial activity of silver nanoparticles , 2012, Bulletin of Materials Science.

[20]  Willy Verstraete,et al.  The antibacterial activity of biogenic silver and its mode of action , 2011, Applied Microbiology and Biotechnology.

[21]  Cristina Rodríguez Padilla,et al.  Bactericidal effect of silver nanoparticles against multidrug-resistant bacteria , 2010 .

[22]  T. Scheper,et al.  Electrochemical method for the synthesis of silver nanoparticles , 2009 .

[23]  G. Mostafa,et al.  Facile synthesis of silver nano particles with highly efficient anti-microbial property , 2007 .

[24]  Arnab Roy,et al.  Characterization of enhanced antibacterial effects of novel silver nanoparticles , 2007, Nanotechnology.

[25]  P. Tam,et al.  Silver nanoparticles: partial oxidation and antibacterial activities , 2007, JBIC Journal of Biological Inorganic Chemistry.

[26]  S. Solomon,et al.  Synthesis and Study of Silver Nanoparticles , 2007 .

[27]  L. Rodríguez-Sánchez,et al.  Electrochemical Synthesis of Silver Nanoparticles , 2000 .

[28]  A. Gupta,et al.  Molecular Genetics: Silver as a biocide: Will resistance become a problem? , 1998, Nature Biotechnology.

[29]  M. Jawaid,et al.  Nanomaterials for Healthcare, Energy and Environment , 2019, Advanced Structured Materials.

[30]  Nanoparticles in Pharmacotherapy , 2019 .

[31]  Kc,et al.  ANTIMICROBIAL ACTIVITY OF COLLOIDAL SILVER NANOPARTICLES PREPARED BY SOL-GEL METHOD , 2011 .

[32]  R. Epand,et al.  Lipid domains in bacterial membranes and the action of antimicrobial agents. , 2009, Biochimica et biophysica acta.

[33]  H. Terryn,et al.  The formation and characterisation of ultra-thin films containing Ag nanoparticles , 2008 .

[34]  V. Wagner,et al.  Efficacy of silver-coated fabric to prevent bacterial colonization and subsequent device-based biofilm formation. , 2000, Journal of biomedical materials research.

[35]  L. Xue Synthesis of Silver Nanoparticles by Electrochemical Method , 2000 .

[36]  Awwa,et al.  Standard Methods for the examination of water and wastewater , 1999 .