Effect of Cold Plasma Treatment on the Functional Properties of Fresh-Cut Apples.

Atmospheric double-barrier discharge (DBD) plasma technology is a promising tool in the food industry as an alternative to traditional food preservation methods. However, the effect of the reactive species generated during the treatment on the content of bioactive compounds in food is still little studied, and there are no data concerning potential deleterious effects of DBD-treated foods on human cells. Some functional properties of DBD-treated minimally processed Pink Lady apples were evaluated in comparison with untreated samples through different in vitro and ex vivo tests. Plasma treatment caused only a slight reduction of antioxidant content and antioxidant capacity (up to 10%), mainly limited to the amphiphilic fraction. Noteworthy, treated apple polyphenol extracts did not reduce cell viability and did not suppress the beneficial physiological cell response to oxidative stress in terms of reactive oxygen species production and phase II enzyme activation in human cultured colonocytes.

[1]  C. Riponi,et al.  Improved HPLC determination of phenolic compounds in cv. golden delicious apples using a monolithic column. , 2004, Journal of agricultural and food chemistry.

[2]  Silvia Tappi,et al.  Effect of cold plasma treatment on physico-chemical parameters and antioxidant activity of minimally processed kiwifruit , 2015 .

[3]  Brendan A. Niemira,et al.  Cold plasma decontamination of foods. , 2012, Annual review of food science and technology.

[4]  G. Duthie,et al.  Role of dietary pro-oxidants in the maintenance of health and resilience to oxidative stress. , 2015, Molecular nutrition & food research.

[5]  Young Jun Kim,et al.  Major phenolics in apple and their contribution to the total antioxidant capacity. , 2003, Journal of agricultural and food chemistry.

[6]  F. Stampar,et al.  Phenolic compounds in some apple (Malus domestica Borkh) cultivars of organic and integrated production , 2005 .

[7]  J. Roth,et al.  An overview of research using the one atmosphere uniform glow discharge plasma (OAUGDP) for sterilization of surfaces and materials , 2000 .

[8]  H. Babich,et al.  Research Strategies in the Study of the Pro-Oxidant Nature of Polyphenol Nutraceuticals , 2011, Journal of toxicology.

[9]  P. Cullen,et al.  Kinetics of tomato peroxidase inactivation by atmospheric pressure cold plasma based on dielectric barrier discharge , 2013 .

[10]  A. Lampen,et al.  Glutathione S-transferase expression and isoenzyme composition during cell differentiation of Caco-2 cells. , 2009, Toxicology.

[11]  L. Stivala,et al.  Flavonoids uptake and their effect on cell cycle of human colon adenocarcinoma cells (Caco2) , 2002, British Journal of Cancer.

[12]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[13]  W. Berger,et al.  EGCG-meditated cyto- and genotoxicity in HaCat keratinocytes is impaired by cell-mediated clearance of auto-oxidation-derived H2O2: an algorithm for experimental setting correction. , 2011, Toxicology letters.

[14]  L. Goya,et al.  Comparative effects of dietary flavanols on antioxidant defences and their response to oxidant-induced stress on Caco2 cells , 2011, European journal of nutrition.

[15]  M. Rosa,et al.  Influence of processing and storage on the antioxidant activity of apple derivatives , 2008 .

[16]  D. Townsend,et al.  Glutathione-s-transferases as determinants of cell survival and death. , 2012, Antioxidants & redox signaling.

[17]  R. Leak,et al.  Emerging roles of Nrf2 and phase II antioxidant enzymes in neuroprotection , 2013, Progress in Neurobiology.

[18]  Ettore Novellino,et al.  Simulated gastrointestinal digestion, intestinal permeation and plasma protein interaction of white, green, and black tea polyphenols. , 2015, Food chemistry.

[19]  R. Prior,et al.  Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. , 2005, Journal of agricultural and food chemistry.

[20]  António A. Vicente,et al.  Environmental impact of novel thermal and non-thermal technologies in food processing , 2010 .

[21]  C Roland Wolf,et al.  Generation of a stable antioxidant response element-driven reporter gene cell line and its use to show redox-dependent activation of nrf2 by cancer chemotherapeutic agents. , 2006, Cancer research.

[22]  F. Rizzi,et al.  Polyphenon E(R), a standardized green tea extract, induces endoplasmic reticulum stress, leading to death of immortalized PNT1a cells by anoikis and tumorigenic PC3 by necroptosis. , 2014, Carcinogenesis.

[23]  G. Eisenbrand,et al.  Polyphenolic apple extracts: effects of raw material and production method on antioxidant effectiveness and reduction of DNA damage in Caco-2 cells. , 2010, Journal of Agricultural and Food Chemistry.

[24]  R. Hurrell,et al.  Identification of apples rich in health-promoting flavan-3-ols and phenolic acids by measuring the polyphenol profile , 2012 .

[25]  Adriano Guarnieri,et al.  Atmospheric gas plasma treatment of fresh-cut apples , 2014 .

[26]  D. Knorr,et al.  Interactions of Non-Thermal Atmospheric Pressure Plasma with Solid and Liquid Food Systems: A Review , 2015, Food Engineering Reviews.

[27]  D. Alberts,et al.  Phase I pharmacokinetic study of tea polyphenols following single-dose administration of epigallocatechin gallate and polyphenon E. , 2001, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[28]  A. Bendini,et al.  Radical scavenging activities of peels and pulps from cv. Golden Delicious apples as related to their phenolic composition. , 2004, Journal of agricultural and food chemistry.

[29]  Michael G. Kong,et al.  Protein destruction by atmospheric pressure glow discharges , 2007 .

[30]  N. Habermann,et al.  Apple polyphenols modulate expression of selected genes related to toxicological defence and stress response in human colon adenoma cells , 2008, International journal of cancer.

[31]  D. Knorr,et al.  Cold plasma effects on enzyme activity in a model food system , 2013 .

[32]  J. Ehlbeck,et al.  Treating lamb's lettuce with a cold plasma ― Influence of atmospheric pressure Ar plasma immanent species on the phenolic profile of Valerianella locusta , 2011 .

[33]  L. Goya,et al.  Dietary flavanols exert different effects on antioxidant defenses and apoptosis/proliferation in Caco-2 and SW480 colon cancer cells. , 2011, Toxicology in Vitro.

[34]  A. Porter,et al.  Nitric Oxide-induced Transcriptional Up-regulation of Protective Genes by Nrf2 via the Antioxidant Response Element Counteracts Apoptosis of Neuroblastoma Cells* , 2004, Journal of Biological Chemistry.

[35]  Jie Yang,et al.  Chemoprevention of dietary digitoflavone on colitis-associated colon tumorigenesis through inducing Nrf2 signaling pathway and inhibition of inflammation , 2014, Molecular Cancer.

[36]  C. Montanari,et al.  Non-thermal atmospheric gas plasma device for surface decontamination of shell eggs , 2010 .

[37]  Shuji Ohno,et al.  Determination of mRNA Expression of Human UDP-Glucuronosyltransferases and Application for Localization in Various Human Tissues by Real-Time Reverse Transcriptase-Polymerase Chain Reaction , 2009, Drug Metabolism and Disposition.

[38]  D. Alberts,et al.  Effects of Dosing Condition on the Oral Bioavailability of Green Tea Catechins after Single-Dose Administration of Polyphenon E in Healthy Individuals , 2005, Clinical Cancer Research.

[39]  E. Takai,et al.  Protein Inactivation by Low‐temperature Atmospheric Pressure Plasma in Aqueous Solution , 2012 .