BIOCHEMICAL STUDIES ON BLACK GRAM (PHASEOLUS MUNGO): I. Solubilization and Electrophoretic Characterization of The Proteins.

Protein content of 60 mesh, dehydrated, defatted black gram (Phaseolus mungo) meal was 28.5%. Sodium carbonate (0.5–1.0%), te trasodium pyrophosphate (0.5%), and sodium dodecyl sulfate (0.5–5%) extracted more than 76 gram Lowry's protein per 100 gram Kjeldahl protein. On the considerations of contaminating residue in the final product and disruption of native structure of the proteins these chemical agents were unsuitable. Sodium sulfate at 10% level was judged to be the best protein solubilizer. The phenol-acetic acid-mercaptoethanol-urea (PAMU) system in polyacrylamide gel electrophoresis was more efficient in resolving protein subunits than the sodium dodecyl sulfate (SDS) gel system. PAMU dissociated total black gram proteins in one major and 7–8 other sharp bands. Proteins separated on PAMU gel were run on the flat bed polyacrylamide gel containing SDS system. The proteins were then separated in 13 subunits, the molecular weight of the major one was 55,000. Defatting with n-hexane improved protein extraction by 6%. Solubilized proteins contained 81% globulins, 13% albumins, 4% ethanol soluble proteins, and 2% acetic acid soluble proteins.

[1]  L. Thompson,et al.  Isolation of Rapeseed Protein Using Sodium Hexametaphosphate , 1976 .

[2]  D. Boulter,et al.  Legumin and vicilin, storage proteins of legume seeds , 1976 .

[3]  T. Hall,et al.  Solubility characteristics of globulins from Phaseolus seeds in regard to their isolation and characterization. , 1975, Journal of agricultural and food chemistry.

[4]  R. Breidenbach,et al.  Proteins of soybean seeds: I. Isolation and characterization of the major components. , 1974, Plant physiology.

[5]  J. Kruseman,et al.  Recovery of whey proteins with sodium hexametaphosphate , 1973 .

[6]  S. Mizrahi,et al.  Effect of processing conditions on isolation of cottonseed protein by sodium hexametaphosphate extraction method , 1973 .

[7]  J. C. Woodard,et al.  Toxicity of alkali-treated soyprotein in rats. , 1973, The Journal of nutrition.

[8]  D. Boulter,et al.  A comparison of acid extracted globulin fractions and vicilin and legumin of Vicia faba , 1973 .

[9]  T. Hall,et al.  Comparison of globulin proteins from Phaseolus vulgaris with those from Vicia faba , 1973 .

[10]  B. Koury,et al.  Phosphate complexes of soluble fish proteins. Their formation and possible uses. , 1970, Journal of agricultural and food chemistry.

[11]  W. Bushuk,et al.  NATURE OF PROTEINS IN TRITICALE AND ITS PARENTAL SPECIES: II. GEL FILTRATION AND DISC ELECTROPHORESIS RESULTS , 1970 .

[12]  W. Bushuk,et al.  NATURE OF PROTEINS IN TRITICALE AND ITS PARENTAL SPECIES I. SOLUBILITY CHARACTERISTICS AND AMINO ACID COMPOSITION OF ENDOSPERM PROTEINS , 1970 .

[13]  K. Weber,et al.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. , 1969, The Journal of biological chemistry.

[14]  E. W. Meyer,et al.  Protein subunits in dormant and germinating soybean seeds. , 1968, Biochimica et biophysica acta.

[15]  J V Maizel,et al.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. , 1967, Biochemical and biophysical research communications.

[16]  G. Odstrchel,et al.  Kinetic studies on the alkaline decomposition of cystine derivatives and peptides. , 1967, Journal of the American Chemical Society.

[17]  K. Takayama,et al.  Studies on the electron transfer system. LXVII. Polyacrylamide gel electrophoresis of the mitochondrial electron transfer complexes. , 1966, Archives of biochemistry and biophysics.

[18]  A. Pusztai.  Interactions of proteins with other polyelectrolytes in a two-phase system containing phenol and aqueous buffers at various pH values. , 1966, The Biochemical journal.

[19]  E. G. Cole,et al.  Cyanate formation and electrophoretic behavior of proteins in gels containing urea. , 1966, Analytical biochemistry.

[20]  P. Duesberg,et al.  Preparative zone electrophoresis of proteins on polyacrylamide gels in 8 M urea. , 1965, Analytical biochemistry.

[21]  B. Davis.  DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS * , 1964, Annals of the New York Academy of Sciences.

[22]  V. Patwardhan.  Pulses and beans in human nutrition. , 1962, The American journal of clinical nutrition.

[23]  F. R. Earle,et al.  Seed Protein Solubility, Comparison of Solubility Characteristics of Selected Seed Proteins , 1959 .

[24]  C. D. Ball,et al.  The isolation, fractionation and electrophoretic characterization of the globulins of mung bean (Phaseolus aureus). , 1952, Archives of biochemistry and biophysics.

[25]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.