In vivo studies on the utilization of mono-L-aspartyl chlorin (NPe6) for photodynamic therapy.

The in vivo photosensitizing efficacy of mono-L-aspartyl chlorin has been studied by determining the percentage of BALB/c mice cured at varying doses of drug. Using an EMT-6 tumor model, animals received i.p. injections of mono-L-aspartyl chlorin (0.5-100 mg/kg) and then were subsequently exposed to light at 664 nm. Tumor biopsies were taken from selected animals sacrificed at 24 h after treatment and routine histopathological sections made. The other animals remained in the dark for a period of 6 weeks to determine the cure rate. Our results show that mono-L-aspartyl chlorin is an effective tumor localizer that brings about the selective degradation of tumor tissue following light exposure.

[1]  T. Dougherty,et al.  Aspects of the cellular uptake and retention of hematoporphyrin derivative and their correlation with the biological response to PRT in vitro. , 1983, Advances in experimental medicine and biology.

[2]  T. Dougherty,et al.  Photoradiation therapy--clinical and drug advances. , 1983, Advances in experimental medicine and biology.

[3]  J E Kaufman,et al.  Photoradiation therapy for the treatment of malignant tumors. , 1978, Cancer research.

[4]  M. Berns,et al.  Laser photoradiation therapy of cancer following hematoporphyrin sensitization , 1982, Lasers in surgery and medicine.

[5]  M W Berns,et al.  Laser photoradiation therapy of cancer. , 1986, Cancer research.

[6]  T J Dougherty,et al.  Photoradiation in the treatment of recurrent breast carcinoma. , 1979, Journal of the National Cancer Institute.

[7]  J. H. Kinsey,et al.  Endoscopic management of lung cancer with hematoporphyrin derivative phototherapy. , 1982, Mayo Clinic proceedings.

[8]  M. Berns,et al.  Gynecologic uses of photoradiation therapy. , 1984, Progress in clinical and biological research.

[9]  H. Kato,et al.  Hematoporphyrin derivative and laser photoradiation in the treatment of lung cancer. , 1982, Chest.

[10]  M. Berns,et al.  Uptake and localization of HPD and "active fraction" in tissue culture and in serially biopsied human tumors. , 1984, Progress in clinical and biological research.

[11]  Svaasand Lo,et al.  Optical dosimetry for direct and interstitial photoradiation therapy of malignant tumors. , 1984 .

[12]  J. H. Kinsey,et al.  Detection and localization of In situ carcinoma of the bladder with hematoporphyrin derivative. , 1982, Mayo Clinic proceedings.

[13]  T. Dougherty.  Photodynamic therapy (PDT) of malignant tumors. , 1984, Critical reviews in oncology/hematology.