Multitool Platform for Morphology and Nanomechanical Characterization of Biological Samples With Coordinated Self-Sensing Probes

Single-cell studies are extremely important in several fields of research in both molecular and cell biology. Current nanocharacterization techniques based on atomic force microscopy allow researchers to study cells and molecules in unprecedented detail. An important limitation of conventional equipment results from the use of a single probe to measure different parameters of the same sample. To avoid this, here we present a multitool platform based on coordinated self-sensing probes. A first tool, based on quartz tuning fork resonators, is used to image the surface. A second tool, based on a piezoresistive cantilever, is used to measure the nanomechanical properties of the sample. Specific instrumentation circuitry was developed to optimize imaging and force measurement with these probes. Different coordination strategies were studied and a solution was selected that coordinates the nanotools in the microworld and in the nanoworld. Finally, an experiment with biological samples was conducted: the tuning-fork-based tool measured the topography of Escherichia Coli bacterial membranes and the piezoresistive-cantilever-based tool measured their elastic properties.

[1]  Robert D. Grober,et al.  Piezo-electric tuning fork tip—sample distance control for near field optical microscopes , 1995 .

[2]  H. Hansma,et al.  Physical Morphology and Surface Properties of Unsaturated Pseudomonas putida Biofilms , 2000, Journal of bacteriology.

[3]  Gerber,et al.  Atomic Force Microscope , 2020, Definitions.

[4]  P. Dawson,et al.  Chemical etching of optical fibre tips--experiment and model. , 2000, Ultramicroscopy.

[5]  J. Samitier,et al.  Reduced Dimensions Autonomous AFM System for working in Microbiorobotics , 2006, The First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006. BioRob 2006..

[6]  Daniel Y. Abramovitch,et al.  Semi‐automatic tuning of PID gains for atomic force microscopes , 2009 .

[7]  Francesc Pérez-Murano,et al.  Crystalline silicon cantilevers for piezoresistive detection of biomolecular forces , 2008 .

[8]  Wataru Yashiro,et al.  A probe-positioning method with two-dimensional calibration pattern for micro-multi-point probes , 2003 .

[9]  Tip Motion Control and Scanning of a Reorientable Micromanipulator With Axially Located Tip , 2012, IEEE/ASME Transactions on Mechatronics.

[10]  Chia-Hsiang Menq,et al.  Control of a Two-Axis Micromanipulator-Based Scanning Probe System for 2.5-D Nanometrology , 2010, IEEE/ASME Transactions on Mechatronics.

[11]  Yang Gan,et al.  Atomic and subnanometer resolution in ambient conditions by atomic force microscopy , 2009 .

[12]  T. Kenny,et al.  1/f noise considerations for the design and process optimization of piezoresistive cantilevers , 2000, Journal of Microelectromechanical Systems.

[13]  Eileen M. Spain,et al.  Spring constants and adhesive properties of native bacterial biofilm cells measured by atomic force microscopy. , 2008, Colloids and surfaces. B, Biointerfaces.

[14]  M. Salerno,et al.  Tutorial: Mapping Adhesion Forces and Calculating Elasticity in Contact-Mode AFM , 2006 .

[15]  M. Puig-Vidal,et al.  Low-noise Instrumentation for the Measurement of Piezoresistive AFM Cantilever Deflection in Robotic Nanobiocharacterization Applications , 2008, 2008 IEEE Instrumentation and Measurement Technology Conference.

[16]  Sergej Fatikow,et al.  Microrobot System for Automatic Nanohandling Inside a Scanning Electron Microscope , 2007 .

[17]  K. Kendall,et al.  Surface energy and the contact of elastic solids , 1971, Proceedings of the Royal Society of London. A. Mathematical and Physical Sciences.

[18]  C D Woodworth,et al.  Atomic force microscopy detects differences in the surface brush of normal and cancerous cells. , 2009, Nature nanotechnology.

[19]  Santosh Devasia,et al.  A Survey of Control Issues in Nanopositioning , 2007, IEEE Transactions on Control Systems Technology.

[20]  Giorgio Ferrari,et al.  Quantitative nanoscale dielectric microscopy of single-layer supported biomembranes. , 2009, Nano letters.

[21]  Manhee Lee,et al.  Active Q control in tuning-fork-based atomic force microscopy , 2007 .

[22]  J. Loos,et al.  The Art of SPM: Scanning Probe Microscopy in Materials Science , 2005 .

[23]  Saeid Bashash,et al.  Development, analysis and control of a high-speed laser-free atomic force microscope. , 2010, The Review of scientific instruments.

[24]  M. Sitti.  Atomic force microscope probe based controlled pushing for nanotribological characterization , 2004, IEEE/ASME Transactions on Mechatronics.

[25]  J. Sader,et al.  Calibration of rectangular atomic force microscope cantilevers , 1999 .

[26]  Hui Xie,et al.  A versatile atomic force microscope for three-dimensional nanomanipulation and nanoassembly , 2009, Nanotechnology.

[27]  Paolo Facci,et al.  AFM: a versatile tool in biophysics , 2005 .

[28]  P. Lutz,et al.  Development, Modeling, and Control of a Micro-/Nanopositioning 2-DOF Stick–Slip Device , 2009, IEEE/ASME Transactions on Mechatronics.

[29]  Ning Xi,et al.  Probing membrane proteins using atomic force microscopy , 2006, Journal of cellular biochemistry.

[30]  A. Limanskii.  Functionalization of amino-modified probes for atomic force microscopy , 2006 .

[31]  Peter Eaton,et al.  Atomic force microscopy study of the antibacterial effects of chitosans on Escherichia coli and Staphylococcus aureus. , 2008, Ultramicroscopy.

[32]  K Youcef-Toumi,et al.  Use of self-actuating and self-sensing cantilevers for imaging biological samples in fluid , 2009, Nanotechnology.

[33]  Y. Lyubchenko,et al.  Atomic force microscopy of DNA and protein-DNA complexes using functionalized mica substrates. , 2001, Methods in molecular biology.

[34]  H. You,et al.  Atomic force microscopy imaging of living cells: progress, problems and prospects. , 1999, Methods in cell science : an official journal of the Society for In Vitro Biology.

[35]  S. O. R. Moheimani,et al.  Minimizing Scanning Errors in Piezoelectric Stack-Actuated Nanopositioning Platforms , 2008 .

[36]  Josep Samitier,et al.  Implementation of a Multirobot System using Self-sensing Probes for Cell and Molecular Biology Experimental Research , 2009 .

[37]  J Y Peng,et al.  Modeling of Piezoelectric-Driven Stick–Slip Actuators , 2011, IEEE/ASME Transactions on Mechatronics.

[38]  P. Sandoz,et al.  Vibration amplitude of a tip-loaded quartz tuning fork during shear force microscopy scanning. , 2008, The Review of scientific instruments.

[39]  Alicia Casals,et al.  Micro-to-nano optical resolution in a multirobot nanobiocharacterization station , 2009, 2009 IEEE/RSJ International Conference on Intelligent Robots and Systems.

[40]  Manel Puig-Vidal,et al.  Electronic driver with amplitude and quality factor control to adjust the response of quartz tuning fork sensors in atomic force microscopy applications , 2012 .

[41]  Susheng Tan,et al.  Nanoscale compression of polymer microspheres by atomic force microscopy. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[42]  Martin Stark,et al.  A simple and accurate method for calibrating the oscillation amplitude of tuning-fork based AFM sensors. , 2008, Ultramicroscopy.

[43]  D. Pink,et al.  Thickness and Elasticity of Gram-Negative Murein Sacculi Measured by Atomic Force Microscopy , 1999, Journal of bacteriology.

[44]  Ning Xi,et al.  CAD-guided automated nanoassembly using atomic force microscopy-based nonrobotics , 2006, IEEE Trans Autom. Sci. Eng..

[45]  Hui Xie,et al.  Development of a Flexible Robotic System for Multiscale Applications of Micro/Nanoscale Manipulation and Assembly , 2011, IEEE/ASME Transactions on Mechatronics.