Establishment of a new cell line from a woodchuck hepatocellular carcinoma

A new cell line derived from a woodchuck hepatitis surface antigen‐positive woodchuck hepatocellular carcinoma has been established and named T3‐HEP‐W1. This new cell line was established directly from a primary woodchuck hepatocellular carcinoma. Adaptation of the cells to the in vitro culture condition was completed after 3 months, with the doubling time of 24 hr. The morphologic features of the cell by light microscopy were of an epithelial type. The modal chromosome number was 100. Ornithine and tyrosine aminotransferase activities were detected. Production of albumin was negative. Integration of woodchuck hepatitis virus DNA was demonstrated by Southern blot analysis, although the secretion of woodchuck hepatitis surface antigen was not detected. T3‐HEP‐W1 is quite different from the previously reported WH257GE10 cell line and provides another in vitro model for the study of human hepatocellular carcinoma related to hepatitis B virus.

[1]  S. Kaneko,et al.  Stable integration of woodchuck hepatitis virus DNA in transplanted tumors and established tissue culture cells derived from a woodchuck primary hepatocellular carcinoma. , 1986, Cancer research.

[2]  H. Popper.  History of the American association for the study of liver diseases , 1982, Hepatology.

[3]  R. Purcell,et al.  Woodchuck hepatitis and hepatocellular carcinoma: Correlation of histologic with virologic observations , 1981, Hepatology.

[4]  W. Robinson.  GENETIC VARIATION AMONG HEPATITIS B AND RELATED VIRUSES * , 1980, Annals of the New York Academy of Sciences.

[5]  S. Sherlock,et al.  Hepatitis B surface antigen and alpha-fetoprotein secreting human primary liver cell cancer in athymic mice , 1980 .

[6]  Ivan Damjanov,et al.  Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line , 1979, Nature.

[7]  J. Summers,et al.  A virus similar to human hepatitis B virus associated with hepatitis and hepatoma in woodchucks. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[8]  E W Geddes,et al.  Establishment of a continuously growing cell line from primary carcinoma of the liver. , 1976, South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde.

[9]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[10]  C. Milstein,et al.  Continuous cultures of fused cells secreting antibody of predefined specificity , 1975, Nature.

[11]  D. Granner,et al.  [80] Tyrosine aminotransferase (rat liver)☆☆☆ , 1970 .

[12]  N. Katunuma,et al.  Crystallization of ornithine transaminase and its properties. , 1968, Biochemical and biophysical research communications.

[13]  K. Benirschke,et al.  The Somatic Chromosomes of the Northeastern American Woodchuck, Marmota monax , 1963 .

[14]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.