miR-140 promotes proliferation and cartilage differentiation of bone mesenchymal stem cells through inhibition of FGF9

Objective: This paper aimed to investigate miR-140 in promoting bone mesenchymal stem cells (BMSCs) to proliferate via targeting FGF9. Methods: Sixty-six bone marrow specimens from patients with osteoarthritis in the orthopedics department of our hospital from January 2018 to October 2019 were collected. A further 66 bone marrow specimens from healthy people during the same period were selected as controls. qPCR was adopted for detecting miR-140 expression in the two groups of bone marrow specimens. miR-140 overexpression vectors were constructed and then transfected into the BMSCs, whose proliferation, invasion, migration, and apoptosis were observed. Western blot was adopted for detecting cartilage differentiation markers (Sox9 and Has2). The binding site of miR-140 and FGF9 was predicted, and their targeted relationship was detected. Results: miR-140 expression downregulated in osteoarthritis (P<0.05), and its overexpression inhibited BMSCs to proliferate and promoted their apoptosis (P<0.05). Sox9 and Has2 were inhibited (P<0.05). According to dual luciferase reporter gene assay, luciferase activity remarkably reduced when miR-140 bound to FGF9 (P<0.05). Conclusion: miR-140 promotes the proliferation and cartilage differentiation of BMSCs via inhibiting FGF9.

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