The Binding Specificity of OppA Determines the Selectivity of the Oligopeptide ATP-binding Cassette Transporter*

The purification and functional reconstitution of a five-component oligopeptide ATP-binding cassette transporter with a remarkably wide substrate specificity are described. High-affinity peptide uptake was dependent on liganded substrate-binding protein OppA, which interacts with the translocator OppBCDF with higher affinity than unliganded OppA. Transport screening with combinatorial peptide libraries revealed that (i) the Opp transporter is not selective with respect to amino acid side chains of the transported peptides; (ii) any peptide that can bind to OppA is transported via Opp, including very long peptides up to 35 residues long; and (iii) the binding specificity of OppA largely determines the overall transport selectivity.

[1]  R. Tampé,et al.  Peptides Induce ATP Hydrolysis at Both Subunits of the Transporter Associated with Antigen Processing* , 2003, Journal of Biological Chemistry.

[2]  B. Poolman,et al.  The ATP/Substrate Stoichiometry of the ATP-binding Cassette (ABC) Transporter OpuA* , 2003, Journal of Biological Chemistry.

[3]  V. Juillard,et al.  Charged casein-derived oligopeptides competitively inhibit the transport of a reporter oligopeptide by Lactococcus lactis. , 2003, Journal of applied microbiology.

[4]  V. Juillard,et al.  Diversity of Oligopeptide Transport Specificity in Lactococcus lactis Species , 2003, The Journal of Biological Chemistry.

[5]  Dirk-Jan Slotboom,et al.  Lactococcus lactis as host for overproduction of functional membrane proteins. , 2003, Biochimica et biophysica acta.

[6]  B. Poolman,et al.  ABC transporters: one, two or four extracytoplasmic substrate‐binding sites? , 2002, EMBO reports.

[7]  G. Robillard,et al.  Combined in-gel tryptic digestion and CNBr cleavage for the generation of peptide maps of an integral membrane protein with MALDI-TOF mass spectrometry. , 2002, Biochimica et biophysica acta.

[8]  Douglas C. Rees,et al.  The E. coli BtuCD Structure: A Framework for ABC Transporter Architecture and Mechanism , 2002, Science.

[9]  B. Poolman,et al.  Peptides and ATP binding cassette peptide transporters. , 2001, Research in microbiology.

[10]  C. Higgins,et al.  ABC transporters: physiology, structure and mechanism--an overview. , 2001, Research in microbiology.

[11]  R. Tampé,et al.  Combinatorial peptide libraries reveal the ligand-binding mechanism of the oligopeptide receptor OppA of Lactococcus lactis. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[12]  B. Poolman,et al.  On the binding mechanism of the peptide receptor of the oligopeptide transport system of Lactococcus lactis , 2000, The EMBO journal.

[13]  B. Poolman,et al.  Wil N. Konings and Bert Poolman of the Oligopeptide Transport System of Specificity Mutants of the Binding Protein , 1999 .

[14]  B. Poolman,et al.  Kinetics and consequences of binding of nona- and dodecapeptides to the oligopeptide binding protein (OppA) of Lactococcus lactis. , 1999, Biochemistry.

[15]  Edmund R. S. Kunji,et al.  Kinetics and specificity of peptide uptake by the oligopeptide transport system of Lactococcus lactis. , 1998, Biochemistry.

[16]  S Uebel,et al.  Recognition principle of the TAP transporter disclosed by combinatorial peptide libraries. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[17]  G. Venema,et al.  Fate of peptides in peptidase mutants of Lactococcus lactis , 1996, Molecular microbiology.

[18]  B. Poolman,et al.  Unidirectional Reconstitution into Detergent-destabilized Liposomes of the Purified Lactose Transport System of Streptococcus thermophilus* , 1996, The Journal of Biological Chemistry.

[19]  K. Nikaido,et al.  Liganded and Unliganded Receptors Interact with Equal Affinity with the Membrane Complex of Periplasmic Permeases, a Subfamily of Traffic ATPases* , 1996, The Journal of Biological Chemistry.

[20]  E. Bohl,et al.  The inhibition of maltose transport by the unliganded form of the maltose-binding protein of Escherichia coli: experimental findings and mathematical treatment. , 1995, Journal of theoretical biology.

[21]  G N Murshudov,et al.  The structural basis of sequence-independent peptide binding by OppA protein. , 1994, Science.

[22]  G. Venema,et al.  Genetic and biochemical characterization of the oligopeptide transport system of Lactococcus lactis , 1993, Journal of bacteriology.

[23]  W. D. de Vos,et al.  Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis. Requirement of expression of the nisA and nisI genes for development of immunity. , 1993, European journal of biochemistry.

[24]  H. Nikaido,et al.  Interaction between maltose‐binding protein and the membrane‐associated maltose transporter complex in Escherichia coli , 1992, Molecular microbiology.

[25]  G. Ames,et al.  Reconstitution of the histidine periplasmic transport system in membrane vesicles. Energy coupling and interaction between the binding protein and the membrane complex. , 1989, The Journal of biological chemistry.