LDDI 32(5).book(LDDI_A_152985.fm)

ABSTRACT A dry extract of Stereum hirsutum exhibited a significant inhibitory activity on thrombin (34%). A bioassay oriented fractionation of the extract of Stereum hirsutum has led to the isolation of two active fractions. On the basis of spectroscopic data, chemical reactions, and GC-MS analysis, complex mixtures of diacylglycerophospholipids (DAGPs) and diacylglycerols (DGs) have been isolated and identified. Docking studies suggest that some isolated compounds could bind to the thrombin active site in a similar manner as previously reported phosphonate thrombin inhibitors.

[1]  P. Larignon,et al.  Fungi associated with esca disease in grapevine , 1997, European Journal of Plant Pathology.

[2]  W. Wienen,et al.  Serine proteases as targets for antithrombotic therapy , 2003 .

[3]  Yangrae Cho,et al.  New tricyclic sesquiterpenes from the fermentation broth of Stereum hirsutum. , 2002, Journal of natural products.

[4]  Yangrae Cho,et al.  Sterins A and B, new antioxidative compounds from Stereum hirsutum. , 2002, The Journal of antibiotics.

[5]  E. Skordalakes,et al.  Inhibition of human alpha-thrombin by a phosphonate tripeptide proceeds via a metastable pentacoordinated phosphorus intermediate. , 2001, Journal of molecular biology.

[6]  C. Coburn.  Small-molecule direct thrombin inhibitors: 1997 - 2000 , 2001 .

[7]  B. Strukelj,et al.  Screening for selective thrombin inhibitors in mushrooms , 2001, Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis.

[8]  R. Tabacchi,et al.  Acetylenic aromatic compounds from Stereum hirsutum. , 2000, Phytochemistry.

[9]  M. Isemura,et al.  The inhibitory properties and primary structure of a novel serine proteinase inhibitor from the fruiting body of the basidiomycete, Lentinus edodes. , 1999, European journal of biochemistry.

[10]  K. Schröder,et al.  Trypsininhibitoraktivität in Basidiomyceten , 1992 .

[11]  Liping Li,et al.  Isolation and identification of two new metabolites from silver leaf fungus Stereum purpureum , 1992 .

[12]  Kenneth Helrich,et al.  Official methods of analysis of the Association of Official Analytical Chemists , 1990 .

[13]  H. Pilgrim,et al.  Untersuchungen zur inhibitorischen Wirkung von wässrigen Extrakten aus Basidiomyceten auf Trypsin , 1985 .

[14]  M. Anchel,et al.  Frustulosinol, an antibiotic metabolite of Stereum frustulosum: Revised structure of frustulosin , 1977 .

[15]  J. Bu’lock,et al.  New benzofurans from Stereum subpileatum, their biosynthesis, and related processes of aromatic aminoacid metabolism in a basidiomycete. , 1971 .