论文信息 - Paternal mitochondria from an rmd-2, rmd-3, rmd-6 triple mutant are properly positioned in the C. elegans zygote

Paternal mitochondria from an rmd-2, rmd-3, rmd-6 triple mutant are properly positioned in the C. elegans zygote

RMD-1,2,3,6 (regulator of microtubule dynamics) is a family of homologous proteins conserved between humans and C. elegans. Human RMD-3/PTPIP51 is a mitochondrial protein that tethers mitochondria to the endoplasmic reticulum. C. elegans RMD-2, 3, and 6 are expressed in sperm. To test whether paternal RMD-2, 3, 6 might redundantly tether paternal mitochondria to maternal ER at fertilization, we generated an rmd-2, rmd-3, rmd-6 triple mutant. Paternal mitochondria derived from control or triple mutant worms were concentrated in a cloud around the paternal DNA at the future posterior end of zygotes during meiosis. No significant difference was detected in the position of paternal mitochondria within the zygote nor in the position of paternal mitochondria relative to paternal DNA within the zygote. There was also no reduction in progeny viability between control and triple mutant worms.

J. McCarthy | F. McNally | Ting Gong | Iris Y Juanico | Christina M Meyer

[1] T. Foster,et al. Mitochondrial Reactive Oxygen Species Generated at the Complex-II Matrix or Intermembrane Space Microdomain Have Distinct Effects on Redox Signaling and Stress Sensitivity in Caenorhabditis elegans. , 2019, Antioxidants & redox signaling.

[2] Andrew W. Folkmann,et al. High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes , 2015, Genetics.

[3] L. Petrucelli,et al. ER–mitochondria associations are regulated by the VAPB–PTPIP51 interaction and are disrupted by ALS/FTD-associated TDP-43 , 2014, Nature Communications.

[4] Fuquan Yang,et al. Characterisation of Caenorhabditis elegans sperm transcriptome and proteome , 2014, BMC Genomics.

[5] C. Shaw,et al. VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis , 2011, Human molecular genetics.

[6] Miyuki Sato,et al. Degradation of Paternal Mitochondria by Fertilization-Triggered Autophagy in C. elegans Embryos , 2011, Science.

[7] H. Okano,et al. RMD-1, a novel microtubule-associated protein, functions in chromosome segregation in Caenorhabditis elegans , 2007, The Journal of cell biology.