TOXICIDADE AGUDA E HISTOPATOLOGIA DO FÍGADO DE LARVAS DE TRAIRÃO (Hoplias lacerdae) EXPOSTAS À SOLUÇÃO AQUOSA DE FORMALDEÍDO A 10

O objetivo deste trabalho foi estimar a concentracao letal 50% (CL (I) 50-96h) da solucao aquosa de formaldeido a 10% para larvas de trairao (Hoplias lacerdae) e avaliar os aspectos histopatologicos no figado das larvas expostas a concentracoes subagudas. Para tanto, realizou-se experimento com seis concentracoes crescentes (0,0; 0,5; 1,0; 1,5; 2,0 e 2,5 mL) de formaldeido. Apos 96 horas de exposicao foram coletadas as larvas sobreviventes de cada tratamento. As larvas foram fixadas em solucao de bouin e incluidas em parafina plastica para a obtencao dos cortes histologicos de 5 µm e coloracao em hematoxilina/eosina. A CL (I) 50-96h para larvas de trairao foi de 2,021 mL/L e a mortalidade de 5%, 10%, 10% e 20% para os tratamentos com 0,5, 1,0, 1,5 e 2,0 mL/L, respectivamente, apos 96 horas de exposicao e de 100% para o tratamento com 2,5 mL/L apos 24 h. Nos tratamentos com 1,0; 1,5 e 2,0 mL/L verificou-se desarranjo cordonal dos hepatocitos, fusao celular e congestao nos sinusoides. A solucao aquosa de formaldeido a 10% pode ser utilizada para terapeutica ou profilaxia em larvas de trairao, em concentracoes de 0,5; 1,0 e 1,5 mL/L, devido a baixa mortalidade dos peixes, porem e necessario alguns cuidados com sua utilizacao devido sua moderada toxicidade aguda.

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