Nutritional physiology of trichothecium roseum

Nutritional physiology of two isolates of Trichothecium roseum was investigated. Optimum temperature and pH for both the isolates were 28 C and 6.0, respectively. D-glucose was the the best substrate for both isolates. There was an increase in mycelial growth as the concentration of D-glucose was increased from 2.5 to 20 g/liter. The amount of growth varied with the nitrogen source, the best growth of the isolate from Mains was on L-asparagine and that of the isolate from Prunus on L-glutamic acid. There was an increase in mycelial growth as the concentration of L-asparagine was increased. There appeared to be no correlation between mycelial growth and the rate of assimilation of amino acid. The presence or absence of amino acids had no correlation with presence or absence of glucose in the medium. The best C/N concentration for maximum growth (D-glucose: L-asparagine) was found to be 30:10.4 g/liter.