SYNCHRONIZA TION OF OVULA TION USING CIDR FOR OV ARIAN CYSTS IN CA TTLE

The objective of this study was to document ovarian and endocrine responses associated with the treatment of ovarian cysts in dairy cattle, using controlled internal drug release (CIDR) regimens. Eight cattle of group-A were administered GnRH analogue (20µg Buserelin) on day 0, PGF 2α (500µg Cloprostenol) on day 7 and 2nd GnRH (20µg Buserelin) on day 9. In addition, CIDR (1.38g progesterone) was inserted on day 0 till day 7. Eight cows of group-B received PGF 2α (500µg Cloprostenol) on day 0, concurrent with the intravaginal placement of CIDR for 15 days followed by GnRH analogue (20µg Buserelin) on day 17. Cattle were inseminated (AI) at 72 hrs after CIDR removal. In group-A, following day 0 treatment, complete luteinization of cystic structure as well as of large follicle occurred in all cattle (p<0.05). In response to day 7 PGF 2α , all the cattle (p<0.05) exhibited a decrease (p<0.05) in plasma progesterone as well as regression (p<0.05) of ovarian cyst / luteinized follicle on day 10. In group-B, day 0 treatment, resulted in considerable decrease (p<0.05) in the diameter of cystic structure and the cyst was no longer detectable by day 15. In both groups, all the cattle (p<0.05) exhibited synchronous ovulation between 7296 hrs after CIDR removal (or 24-48 h after GnRH injection). Moreover, luteal profile on day 6 post-AI as well as conception rate (62.5%) was similar in both the groups. These data suggested that both the CIDR-based timed AI protocols were equally successful for the treatment of ovarian cyst and recruitment of a follicle for synchronous ovulation in dairy cattle with ovarian cysts.

[1]  A. Jain,et al.  Induction of ovulation of ovulatory size non-ovulatory follicles and initiation of ovarian cyclicity in summer anoestrous buffalo heifers (Bubalus bubalis) using melatonin implants. , 2008, Reproduction in domestic animals = Zuchthygiene.

[2]  D. Dadarwal,et al.  Synchronization of ovulation and subsequent fertility in buffaloes following PGF2αC-PGF2α protocol, with or without GnRH. , 2009 .

[3]  N. D. Singh,et al.  Investigations on dominant follicle development, estrus response, ovulation time and fertility in PRID-treated anestrous buffalo heifers. , 2009 .

[4]  G. S. Dhaliwal,et al.  Production of polyclonal antiserum against progesterone for radioimmunoassay. , 2009 .

[5]  Hyun-Gu Kang,et al.  A CIDR-based timed AI protocol can be effectively used for dairy cows with follicular cysts. , 2006, Animal reproduction science.

[6]  D. J. Ambrose,et al.  Ovarian and endocrine responses associated with the treatment of cystic ovarian follicles in dairy cows with gonadotropin releasing hormone and prostaglandin F2alpha, with or without exogenous progesterone. , 2004, The Canadian veterinary journal = La revue veterinaire canadienne.

[7]  A. T. Peter.  An update on cystic ovarian degeneration in cattle. , 2004, Reproduction in domestic animals = Zuchthygiene.

[8]  M. Wiltbank,et al.  An Alteration in the Hypothalamic Action of Estradiol Due to Lack of Progesterone Exposure Can Cause Follicular Cysts in Cattle1 , 2002, Biology of reproduction.

[9]  H. Kaneko,et al.  Restoring ovulation in beef donor cows with ovarian cysts by progesterone-releasing intravaginal silastic devices. , 2001, Theriogenology.

[10]  M. Lucy,et al.  Reproductive loss in high-producing dairy cattle: where will it end? , 2001, Journal of dairy science.

[11]  H. Dobson,et al.  Ultrasound diagnosis and treatment outcome of cystic ovaries in cattle , 2001, Veterinary Record.

[12]  H. Dobson,et al.  Comparison of different methods of diagnosis of cystic ovarian disease in cattle and an assessment of its treatment with a progesterone-releasing intravaginaI device , 2000, Veterinary Record.

[13]  C. Risco,et al.  Comparison of synchronization of ovulation and induction of estrus as therapeutic strategies for bovine ovarian cysts in the dairy cow. , 2000, Theriogenology.

[14]  H. Rodríguez-Martínez,et al.  Ultrastructure of Bovine Ovarian Follicles Induced to Extended Growth by Perioestrous Suprabasal Progesterone Levels , 1996, Acta Veterinaria Scandinavica.