Yeast YAP1 encodes a novel form of the jun family of transcriptional activator proteins.

The jun family of transcriptional activators includes mammalian AP-1 as well as the yeast regulatory protein GCN4. Recently, an additional transcriptional activator has been found in yeast that recognizes the TGACTCA sequence element common in GCN4/AP-1 sites. This factor was designated yAP-1. The structural gene for yAP-1 has now been isolated and characterized. The deduced amino acid sequence predicts a protein of 650 residues, considerably larger than GCN4 or c-Jun. The amino terminus of yAP-1 is homologous to the carboxy-terminal DNA-binding domains of GCN4 and c-Jun. Disruption of the YAP1 gene demonstrates this gene is not essential but is required for AP-1 recognition element-dependent transcriptional activation. DNA-affinity blots of proteins from YAP1 cells suggest the presence of additional TGACTCA-binding proteins other than GCN4 and yAP-1. Furthermore, expression of at least one of these related DNA-binding proteins appears to be under control of yAP-1.

[1]  C. S. Parker,et al.  Isolation of the gene encoding the S. cerevisiae heat shock transcription factor , 1988, Cell.

[2]  A. Sentenac,et al.  A yeast activity can substitute for the HeLa cell TATA box factor , 1988, Nature.

[3]  S. McKnight,et al.  Isolation of a recombinant copy of the gene encoding C/EBP. , 1988, Genes & development.

[4]  S. McKnight,et al.  The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. , 1988, Science.

[5]  R. Tjian,et al.  Fos-associated protein p39 is the product of the jun proto-oncogene. , 1988, Science.

[6]  C. S. Parker,et al.  Transcriptional activation by the SV40 AP-1 recognition element in yeast is mediated by a factor similar to AP-1 that is distinct from GCN4 , 1988, Cell.

[7]  P. Sharp,et al.  Human CCAAT-binding proteins have heterologous subunits , 1988, Cell.

[8]  P. Sharp,et al.  A yeast and a human CCAAT-binding protein have heterologous subunits that are functionally interchangeable , 1988, Cell.

[9]  T. Hunter,et al.  Oncogene jun encodes a sequence-specific trans- activator similar to AP-1 , 1988, Nature.

[10]  B. Franza,et al.  The Fos complex and Fos-related antigens recognize sequence elements that contain AP-1 binding sites. , 1988, Science.

[11]  L. Lau,et al.  A gene activated by growth factors is related to the oncogene v-jun. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[12]  P. Rigby,et al.  Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses. , 1988, Genes & development.

[13]  R. Tjian,et al.  Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1. , 1987, Science.

[14]  Kim Nasmyth,et al.  Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements , 1987, Cell.

[15]  R. Tjian,et al.  Positive and negative regulation of transcription in vitro: Enhancer-binding protein AP-2 is inhibited by SV40 T antigen , 1987, Cell.

[16]  C. Benoist,et al.  A multiplicity of CCAAT box-binding proteins , 1987, Cell.

[17]  K. Struhl.  The DNA-binding domains of the jun oncoprotein and the yeast GCN4 transcriptional activator protein are functionally homologous , 1987, Cell.

[18]  M. Karin,et al.  Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor , 1987, Cell.

[19]  R. Tjian,et al.  Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements , 1987, Cell.

[20]  R. Doolittle,et al.  Homology between the DNA-binding domain of the GCN4 regulatory protein of yeast and the carboxyl-terminal region of a protein coded for by the oncogene jun. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[21]  C. S. Parker,et al.  The saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties , 1987, Cell.

[22]  R. Tjian,et al.  Activation of transcription by two factors that bind promoter and enhancer sequences of the human metallothionein gene and SV40 , 1987, Nature.

[23]  Robert Tjian,et al.  A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication , 1987, Cell.

[24]  R. Young,et al.  [51] Gene isolation by screening λgt11 libraries with antibodies , 1987 .

[25]  M. Rosenberg,et al.  Expression, identification, and characterization of recombinant gene products in Escherichia coli. , 1987, Methods in enzymology.

[26]  K. Struhl,et al.  Saturation mutagenesis of the yeast his3 regulatory site: requirements for transcriptional induction and for binding by GCN4 activator protein. , 1986, Science.

[27]  S. McKnight,et al.  Homologous recognition of a promoter domain common to the MSV LTR and the HSV tk gene , 1986, Cell.

[28]  S. Emr,et al.  The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal , 1986, The Journal of cell biology.

[29]  K. Struhl.  Nucleotide sequence and transcriptional mapping of the yeast pet56-his3-ded1 gene region. , 1985, Nucleic acids research.

[30]  K. Struhl,et al.  GCN4 protein, synthesize in vitro, binds HIS3 regulatory sequences: Implications for general control of amino acid biosynthetic genes in yeast , 1985, Cell.

[31]  F. Ruddle,et al.  Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[32]  C. Yanisch-Perron,et al.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. , 1985, Gene.

[33]  R. Losson,et al.  Yeast regulatory gene PPR1. I. Nucleotide sequence, restriction map and codon usage. , 1984, Journal of molecular biology.

[34]  A. Hinnebusch.  Evidence for translational regulation of the activator of general amino acid control in yeast. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[35]  R. W. Davis,et al.  Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae , 1984, Molecular and cellular biology.

[36]  G. Thireos,et al.  5' untranslated sequences are required for the translational control of a yeast regulatory gene. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[37]  M. Kozak,et al.  Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin. , 1984, Nucleic acids research.

[38]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[39]  G. Hong,et al.  Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[40]  G. Fink,et al.  Repeated DNA sequences upstream from HIS1 also occur at several other co-regulated genes in Saccharomyces cerevisiae. , 1983, The Journal of biological chemistry.

[41]  R. W. Davis,et al.  Efficient isolation of genes by using antibody probes. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[42]  K. Murata,et al.  Transformation of intact yeast cells treated with alkali cations , 1983 .

[43]  G. Fink,et al.  Eviction and transplacement of mutant genes in yeast. , 1983, Methods in enzymology.

[44]  R. Rothstein.  One-step gene disruption in yeast. , 1983, Methods in enzymology.

[45]  J Messing,et al.  A system for shotgun DNA sequencing. , 1981, Nucleic acids research.

[46]  W. Gilbert,et al.  Sequencing end-labeled DNA with base-specific chemical cleavages. , 1980, Methods in enzymology.

[47]  G. Fink,et al.  Methods in yeast genetics , 1979 .

[48]  J. Beggs.  Transformation of yeast by a replicating hybrid plasmid , 1978, Nature.

[49]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.