Application of microfluidic “lab-on-a-chip” for the detection of mycotoxins in foods

Abstract Background Various foods are susceptible to contamination and adulteration with mycotoxins, presenting serious health risks to humans. Microfluidic “lab-on-a-chip” devices could integrate and miniaturize versatile functions from sample preparation to detection, showing great potential in rapid, accurate, and high-throughput detection of mycotoxins. Scope and approach This review focuses on the application of microfluidic “lab-on-a-chip” platforms to detect mycotoxins in foods. Fabrication processes and major components of microfluidic devices, as well as separation and detection methods integrated with “lab-on-a-chip” systems are summarized and discussed. Finally, challenges and future research directions in the development of microfluidic devices to detect mycotoxins are highlighted. Key findings and conclusions Microfluidic “lab-on-a-chip” devices have a great potential for accurate and high-throughput detection of mycotoxins in agricultural and food products.

[1]  M. Zain Impact of mycotoxins on humans and animals , 2011 .

[2]  Alberto Escarpa,et al.  Integrated electrokinetic magnetic bead-based electrochemical immunoassay on microfluidic chips for reliable control of permitted levels of zearalenone in infant foods. , 2011, The Analyst.

[3]  L. Hood,et al.  Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood , 2008, Nature Biotechnology.

[4]  Jie Xu,et al.  Microfluidics “lab‐on‐a‐chip” system for food chemical hazard detection , 2014 .

[5]  Miriam M. Ngundi,et al.  Multiplexed detection of mycotoxins in foods with a regenerable array. , 2006, Journal of food protection.

[6]  Laura Anfossi,et al.  Development and application of a quantitative lateral flow immunoassay for fumonisins in maize. , 2010, Analytica chimica acta.

[7]  Weihong Tan,et al.  Aptamer-based microfluidic device for enrichment, sorting, and detection of multiple cancer cells. , 2009, Analytical chemistry.

[8]  Jie Xu,et al.  Oscillating bubbles in teardrop cavities for microflow control , 2013 .

[9]  Jie Xu,et al.  Use of a porous membrane for gas bubble removal in microfluidic channels: physical mechanisms and design criteria , 2010, 1005.0107.

[10]  A. Roda,et al.  Development and validation of a sensitive and fast chemiluminescent enzyme immunoassay for the detection of genetically modified maize , 2006, Analytical and bioanalytical chemistry.

[11]  Antje J. Baeumner,et al.  Micro-total analysis system for virus detection: microfluidic pre-concentration coupled to liposome-based detection , 2011, Analytical and Bioanalytical Chemistry.

[12]  Salvatore Fanali,et al.  Food analysis: a continuous challenge for miniaturized separation techniques. , 2009, Journal of separation science.

[13]  Tai Hyun Park,et al.  Cell-based microfluidic platform for mimicking human olfactory system. , 2015, Biosensors & bioelectronics.

[14]  Leidong Mao,et al.  Towards ferrofluidics for μ-TAS and lab on-a-chip applications , 2006, Nanotechnology.

[15]  Shuichi Takayama,et al.  Computerized microfluidic cell culture using elastomeric channels and Braille displays. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[16]  G. Palleschi,et al.  Electrochemical immunosensor array using a 96-well screen-printed microplate for aflatoxin B1 detection. , 2007, Biosensors & bioelectronics.

[17]  Robert J. Messinger,et al.  Making it stick: convection, reaction and diffusion in surface-based biosensors , 2008, Nature Biotechnology.

[18]  Britta Ottosson,et al.  LTCC interconnects in microsystems , 2006 .

[19]  Bart Nicolai,et al.  Microfluidic analytical systems for food analysis , 2011 .

[20]  Rosangela Marchelli,et al.  Recent advances in mycotoxin determination in food and feed by hyphenated chromatographic techniques/mass spectrometry. , 2006, Mass spectrometry reviews.

[21]  Chien-Chong Hong,et al.  A disposable microfluidic biochip with on-chip molecularly imprinted biosensors for optical detection of anesthetic propofol. , 2010, Biosensors & bioelectronics.

[22]  S. Quake,et al.  Microfluidics: Fluid physics at the nanoliter scale , 2005 .

[23]  Sungyon Lee,et al.  Manipulation of biological objects using acoustic bubbles: a review. , 2014, Integrative and comparative biology.

[24]  Wei Ting Chen,et al.  Development of an immunoassay based on impedance measurements utilizing an antibody-nanosilver probe, silver enhancement, and electro-microchip , 2009 .

[25]  Juan G. Santiago,et al.  A review of micropumps , 2004 .

[26]  Jan Roelof van der Meer,et al.  Development of a Set of Simple Bacterial Biosensors for Quantitative and Rapid Measurements of Arsenite and Arsenate in Potable Water. , 2004, Environmental Science and Technology.

[27]  Sarah De Saeger,et al.  Development of a multi-mycotoxin liquid chromatography/tandem mass spectrometry method for sweet pepper analysis. , 2009, Rapid communications in mass spectrometry : RCM.

[28]  Andres W. Martinez,et al.  Fully enclosed microfluidic paper-based analytical devices. , 2012, Analytical chemistry.

[29]  Tamás Nepusz,et al.  Network analysis of the RASFF database: a mycotoxin perspective , 2011 .

[30]  Dmitriy A. Khodakov,et al.  Surface modification for PDMS‐based microfluidic devices , 2012, Electrophoresis.

[31]  Guocheng Shao,et al.  Microfabricated renewable beads-trapping/releasing flow cell for rapid antigen-antibody reaction in chemiluminescent immunoassay. , 2011, Analytical chemistry.

[32]  A. Folch Introduction to BioMEMS , 2012 .

[33]  G. Whitesides,et al.  Diagnostics for the developing world: microfluidic paper-based analytical devices. , 2010, Analytical chemistry.

[34]  T. Bayraktar,et al.  Characterization of liquid flows in microfluidic systems , 2006 .

[35]  Eun Kyu Lee,et al.  Continuous dynamic flow micropumps for microfluid manipulation , 2007 .

[36]  J. Richard,et al.  Some major mycotoxins and their mycotoxicoses--an overview. , 2007, International journal of food microbiology.

[37]  P Novo,et al.  On-chip sample preparation and analyte quantification using a microfluidic aqueous two-phase extraction coupled with an immunoassay. , 2014, Lab on a chip.

[38]  Reinhard Niessner,et al.  Surface-enhanced Raman scattering-based label-free microarray readout for the detection of microorganisms. , 2010, Analytical chemistry.

[39]  Jianping Fu,et al.  Elastomeric microposts integrated into microfluidics for flow-mediated endothelial mechanotransduction analysis. , 2012, Lab on a chip.

[40]  Olof Ramström,et al.  The Emerging Technique of Molecular Imprinting and Its Future Impact on Biotechnology , 1996, Bio/Technology.

[41]  Baohong Liu,et al.  Microfluidic immunosensor based on stable antibody-patterned surface in PMMA microchip , 2008 .

[42]  Michael G. Roper,et al.  Recent advances in microfluidic detection systems. , 2009, Bioanalysis.

[43]  M. Zheng,et al.  A Review of Rapid Methods for the Analysis of Mycotoxins , 2006, Mycopathologia.

[44]  S Pennathur,et al.  Flow control in microfluidics: are the workhorse flows adequate? , 2008, Lab on a chip.

[45]  Daniel Ahmed,et al.  A millisecond micromixer via single-bubble-based acoustic streaming. , 2009, Lab on a chip.

[46]  Shu-Ling Lin,et al.  Microfluidic chip-based nano-liquid chromatography tandem mass spectrometry for quantification of aflatoxins in peanut products. , 2013, Talanta.

[47]  Zhaowei Zhang,et al.  Current development of microfluidic immunosensing approaches for mycotoxin detection via capillary electromigration and lateral flow technology , 2012, Electrophoresis.

[48]  François Lagugné-Labarthet,et al.  Microfluidic channel with embedded SERS 2D platform for the aptamer detection of ochratoxin A , 2013, Analytical and Bioanalytical Chemistry.

[49]  Shuichi Takayama,et al.  Handheld recirculation system and customized media for microfluidic cell culture. , 2006, Lab on a chip.

[50]  Greg E Collins,et al.  Microchip micellar electrokinetic chromatography separation of alkaloids with UV‐absorbance spectral detection , 2008, Electrophoresis.

[51]  D. Beebe,et al.  Physics and applications of microfluidics in biology. , 2002, Annual review of biomedical engineering.

[52]  Julio Raba,et al.  Citrinin (CIT) determination in rice samples using a micro fluidic electrochemical immunosensor. , 2011, Talanta.

[53]  Gwo-Bin Lee,et al.  A microfluidic system with integrated molecular imprinting polymer films for surface plasmon resonance detection , 2006 .

[54]  Alberto Escarpa,et al.  Electrochemical immunosensing on board microfluidic chip platforms , 2012 .

[55]  Nam-Trung Nguyen,et al.  Micromixers?a review , 2005 .

[56]  Hans P. van Egmond,et al.  Regulations relating to mycotoxins in food , 2007 .

[57]  M Castegnaro,et al.  Balkan endemic nephropathy and associated urinary tract tumours: a review on aetiological causes and the potential role of mycotoxins , 2002, Food additives and contaminants.

[58]  Adam T. Melvin,et al.  Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field. , 2013, Analytical chemistry.

[59]  Weihong Tan,et al.  Aptamer-enabled efficient isolation of cancer cells from whole blood using a microfluidic device. , 2012, Analytical chemistry.

[60]  Meng Hua,et al.  Nanograssed Micropyramidal Architectures for Continuous Dropwise Condensation , 2011 .

[61]  Youjun Deng,et al.  Microfluidic Smectite-Polymer Nanocomposite Strip Sensor for Aflatoxin Detection , 2013, IEEE Sensors Journal.

[62]  Shenguang Ge,et al.  A disposable immunosensor device for point-of-care test of tumor marker based on copper-mediated amplification. , 2013, Biosensors & bioelectronics.

[63]  Chao Lin,et al.  Development of a one-step test strip for rapid screening of fumonisins B1, B2 and B3 in maize , 2012 .

[64]  Wei Duan,et al.  Lab-on-a-chip: a component view , 2010 .

[65]  D. Di Carlo Inertial microfluidics. , 2009, Lab on a chip.

[66]  S. Quake,et al.  Monolithic microfabricated valves and pumps by multilayer soft lithography. , 2000, Science.

[67]  Ioannis S. Arvanitoyannis,et al.  Mycotoxins in Food: Detection and Control , 2005 .

[68]  Chong H. Ahn,et al.  Institute of Physics Publishing Journal of Micromechanics and Microengineering a Review of Microvalves , 2022 .

[69]  Daniel Ahmed,et al.  Tunable, pulsatile chemical gradient generation via acoustically driven oscillating bubbles. , 2013, Lab on a chip.

[70]  A. Lee,et al.  Droplet microfluidics. , 2008, Lab on a chip.

[71]  Miriam M. Ngundi,et al.  Array biosensor for detection of ochratoxin A in cereals and beverages. , 2005, Analytical chemistry.

[72]  A. deMello Control and detection of chemical reactions in microfluidic systems , 2006, Nature.

[73]  Howard A. Stone,et al.  ENGINEERING FLOWS IN SMALL DEVICES , 2004 .

[74]  Chayma Bouaziz,et al.  The in vitro effects of zearalenone and T-2 toxins on Vero cells. , 2013, Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie.

[75]  L. Locascio,et al.  Integrated plastic microfluidic devices with ESI-MS for drug screening and residue analysis. , 2001, Analytical chemistry.

[76]  S. Herminghaus,et al.  Droplet based microfluidics , 2012, Reports on progress in physics. Physical Society.

[77]  Yordan Kostov,et al.  An automated point-of-care system for immunodetection of staphylococcal enterotoxin B. , 2011, Analytical biochemistry.

[78]  Reinhard Niessner,et al.  Regenerable immuno-biochip for screening ochratoxin A in green coffee extract using an automated microarray chip reader with chemiluminescence detection. , 2011, Analytica chimica acta.

[79]  João Pedro Conde,et al.  Lab-on-a-Chip Ochratoxin A Detection Using Competitive ELISA in Microfluidics with Integrated Photodiode Signal Acquisition , 2011 .

[80]  Theodore P. Labuza,et al.  Aptamer-based surface-enhanced Raman scattering detection of ricin in liquid foods , 2011 .

[81]  N. Voelcker,et al.  Recent developments in PDMS surface modification for microfluidic devices , 2010, Electrophoresis.

[82]  I. Tothill,et al.  Electrochemical immunochip sensor for aflatoxin M1 detection. , 2009, Analytical chemistry.

[83]  Alejandro Garcia-Uribe,et al.  Layer-by-layer assembled smectite-polymer nanocomposite film for rapid fluorometric detection of aflatoxin B1 , 2012 .

[84]  G. Whitesides The origins and the future of microfluidics , 2006, Nature.

[85]  H. S. Hussein,et al.  Toxicity, metabolism, and impact of mycotoxins on humans and animals. , 2001, Toxicology.

[86]  M. A. Jonker,et al.  Worldwide regulations for mycotoxins in food and feed in 2003 , 2004 .

[87]  Achim Wixforth,et al.  Acoustic mixing at low Reynold's numbers , 2006 .

[88]  George M. Whitesides,et al.  Fabrication of Low-Cost Paper-Based Microfluidic Devices by Embossing or Cut-and-Stack Methods , 2014 .

[89]  R. Zengerle,et al.  Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications. , 2010, Chemical Society reviews.

[90]  A. Manz,et al.  Micro total analysis systems. Latest advancements and trends. , 2006, Analytical chemistry.

[91]  Reinhard Niessner,et al.  Automated analytical microarrays: a critical review , 2008, Analytical and bioanalytical chemistry.

[92]  A. Heeger,et al.  Micromagnetic selection of aptamers in microfluidic channels , 2009, Proceedings of the National Academy of Sciences.

[93]  J. Lammertyn,et al.  Biofunctionalization of electrowetting-on-dielectric digital microfluidic chips for miniaturized cell-based applications. , 2011, Lab on a chip.

[94]  Chunsheng Wu,et al.  Cell-based biosensors and their application in biomedicine. , 2014, Chemical reviews.

[95]  林炳承,et al.  Patterned Paper as a Low-cost, Flexible Substrate for Rapid Prototyping PDMS Microdevices via “liquid molding” , 2011 .

[96]  J. Voldman,et al.  An integrated liquid mixer/valve , 2000, Journal of Microelectromechanical Systems.

[97]  V. Lattanzio,et al.  Simultaneous determination of aflatoxins, ochratoxin A and Fusarium toxins in maize by liquid chromatography/tandem mass spectrometry after multitoxin immunoaffinity cleanup. , 2007, Rapid communications in mass spectrometry : RCM.

[98]  Wei-Ming Yeh,et al.  A microfluidic system utilizing molecularly imprinted polymer films for amperometric detection of morphine , 2007 .

[99]  V. Chu,et al.  Detection of ochratoxin A in wine and beer by chemiluminescence-based ELISA in microfluidics with integrated photodiodes , 2013 .

[100]  Won-Bo Shim,et al.  One-step simultaneous immunochromatographic strip test for multianalysis of ochratoxin a and zearalenone. , 2009, Journal of microbiology and biotechnology.

[101]  Michael V. Pishko,et al.  Cell-based bioassays in microfluidic systems , 2004, SPIE Optics East.

[102]  Amy Rachel Betz,et al.  Microfluidic formation of monodispersed spherical microgels composed of triple‐network crosslinking , 2011 .

[103]  Jie Xu,et al.  Microbubble array for on-chip worm processing , 2013 .

[104]  D. Dietrich,et al.  Ochratoxin A: The Continuing Enigma , 2005, Critical reviews in toxicology.

[105]  Albert Folch,et al.  Microvalves and Micropumps for BioMEMS , 2011, Micromachines.

[106]  Jie Xu,et al.  Drop on demand in a microfluidic chip , 2008, 0912.2905.

[107]  M. A. Jonker,et al.  Regulations relating to mycotoxins in food: perspectives in a global and European context. , 2007, Analytical and bioanalytical chemistry.

[108]  A. T. Giannitsis,et al.  Fabrication methods for microfluidic lab-on-chips , 2010, 2010 12th Biennial Baltic Electronics Conference.

[109]  U. Karst,et al.  Quantitative analysis by microchip capillary electrophoresis: current limitations and problem-solving strategies. , 2008, The Analyst.

[110]  Steven J. Lehotay,et al.  Application of gas chromatography in food analysis , 2002 .

[111]  R. Manderville,et al.  Structure-activity relationships for the fluorescence of ochratoxin A: insight for detection of ochratoxin A metabolites. , 2008, Analytica chimica acta.

[112]  Jianping Fu,et al.  Photolithographic surface micromachining of polydimethylsiloxane (PDMS). , 2012, Lab on a chip.

[113]  Leonard Stoloff,et al.  Occurrence of Mycotoxins in Foods and Feeds , 1976 .

[114]  Jie Xu,et al.  On the Quantification of Mixing in Microfluidics , 2014, Journal of laboratory automation.

[115]  Robert T Kennedy,et al.  Fully integrated microfluidic separations systems for biochemical analysis. , 2007, Journal of chromatography. A.

[116]  D. Beebe,et al.  The present and future role of microfluidics in biomedical research , 2014, Nature.

[117]  Ibtisam E. Tothill,et al.  Emerging bio-sensing methods for mycotoxin analysis , 2011 .

[118]  David J. Beebe,et al.  A microscale neuron and Schwann cell coculture model for increasing detection sensitivity of botulinum neurotoxin type A. , 2013, Toxicological sciences : an official journal of the Society of Toxicology.

[119]  Babak Ziaie,et al.  A magnetically driven PDMS micropump with ball check-valves , 2005 .

[120]  Nicolas Marquestaut,et al.  Plasmonic properties of Fischer's patterns: polarization effects. , 2010, Physical chemistry chemical physics : PCCP.

[121]  Zuankai Wang,et al.  Nanostructured silver nanowires-graphene hybrids for enhanced electrochemical detection of hydrogen peroxide , 2013 .