The two-exon gene of the human forkhead transcription factor FREAC-2 (FKHL6) is located at 6p25.3.

The gene for the human transcription factor forkhead related activator 2 (FREAC-2; HGMW-approved symbol FKHL6) has been characterized and found to consist of two exons separated by an intron of 3.6 kb. The first exon encodes the forkhead DNA-binding domain and one of the transcriptional activation domains, AD2. The second exon contains the coding sequence corresponding to the C-terminal activation domain AD1. The full-length FREAC-2 protein is predicted to be 444 amino acids, which adds 39 amino acids to the previously published partial cDNA sequence. A 2-kb CG island is centered around the 5' end of the FREAC-2 gene. Fluorescence in situ hybridization was used to localize the human FREAC-2 gene to chromosomal position 6p24-p25, and the localization was further refined by radiation hybrid mapping to 6p25.3.

[1]  P. Carlsson,et al.  FREAC-1 contains a cell-type-specific transcriptional activation domain and is expressed in epithelial-mesenchymal interfaces. , 1998, Developmental biology.

[2]  P. Carlsson,et al.  The Human Forkhead Protein FREAC-2 Contains Two Functionally Redundant Activation Domains and Interacts with TBP and TFIIB* , 1998, The Journal of Biological Chemistry.

[3]  C. Larsson,et al.  Cloning and characterization of freac-9 (FKHL17), a novel kidney-expressed human forkhead gene that maps to chromosome 1p32-p34. , 1997, Genomics.

[4]  M. Hulander,et al.  Characterization of the Human Forkhead Gene FREAC-4 , 1996, The Journal of Biological Chemistry.

[5]  W. Knöchel,et al.  Five years on the wings of fork head , 1996, Mechanisms of Development.

[6]  Cécile Fizames,et al.  A comprehensive genetic map of the human genome based on 5,264 microsatellites , 1996, Nature.

[7]  P. Carlsson,et al.  Differential Activation of Lung-specific Genes by Two Forkhead Proteins, FREAC-1 and FREAC-2 (*) , 1996, The Journal of Biological Chemistry.

[8]  C. Larsson,et al.  Chromosomal localization of six human forkhead genes, freac-1 (FKHL5), -3 (FKHL7), -4 (FKHL8), -5 (FKHL9), -6 (FKHL10), and -8 (FKHL12). , 1995, Genomics.

[9]  P. Carlsson,et al.  Selection of high-affinity binding sites for sequence-specific, DNA binding proteins from random sequence oligonucleotides. , 1995, Analytical biochemistry.

[10]  P. Carlsson,et al.  Cloning and characterization of seven human forkhead proteins: binding site specificity and DNA bending. , 1994, The EMBO journal.

[11]  Yalin H. Wei,et al.  Cloning and characterization of the t(X;II) breakpoint from a leukemic cell line identify a new member of the forkhead gene family , 1994, Genes, chromosomes & cancer.

[12]  J. Downing,et al.  Fusion of PAX3 to a member of the forkhead family of transcription factors in human alveolar rhabdomyosarcoma. , 1993, Cancer research.

[13]  B. Dutrillaux,et al.  A simple method for simultaneous R- or G-banding and fluorescence in situ hybridization of small single-copy genes. , 1992, Cytogenetics and cell genetics.

[14]  P. Sharp,et al.  Splicing of messenger RNA precursors. , 1987, Science.