Changes in electrical impedance of biological matter due to the application of ultrashort high voltage pulses

Time domain-based impedance measurements were used to study the changes in electrical parameters of biological samples following the application of ultrashort high voltage (HV) pulses. Pulses with very short duration (300 ns) caused a significant drop in post pulse resistance of the plasma membrane only at high field strengths in excess of 20 kV/cm. The conductivity of the plasma membrane returned to almost pre-pulse values within less than 10 ms after the field was applied. Further steps of recovery, attributed to pore shrinking and resealing with an exponential decay of the conductivity, as is expected in electroporation, were not observed. An increase in medium conductance, as recorded minutes after the pulse, arises mainly from cell damage. Although pore formation is a possible effect of the high electric field, our results suggest further disturbance of the membrane-like micelle formation or even the creation of large defects, forced by mechanical tension within the cell membrane.

[1]  H. Schwan.  Electrical properties of tissue and cell suspensions. , 1957, Advances in biological and medical physics.

[2]  R. Cole,et al.  Time domain reflectometry. , 1977, Annual review of physical chemistry.

[3]  L. Mir,et al.  Electrochemotherapy, a new antitumor treatment. First clinical phase I‐II trial , 1993, Cancer.

[4]  R. Cole,et al.  Evaluation of dielectric behavior by time domain spectroscopy. 3. Precision difference methods , 1980 .

[5]  S. Bone,et al.  Time domain reflectrometry: the difference method applied to conductive aqueous solutions. , 1988, Biochimica et biophysica acta.

[6]  Michael R Murphy,et al.  Plasma membrane permeabilization by 60‐ and 600‐ns electric pulses is determined by the absorbed dose , 2009, Bioelectromagnetics.

[7]  J. Weaver,et al.  Theory of electroporation: A review , 1996 .

[8]  E. Neumann,et al.  Electroporation and Electrofusion in Cell Biology , 1989, Springer US.

[9]  L. Chernomordik,et al.  Reversible electrical breakdown of lipid bilayers: formation and evolution of pores. , 1988, Biochimica et biophysica acta.

[10]  H. Pauly,et al.  Über die Impedanz einer Suspension von kugelförmigen Teilchen mit einer Schale , 1959 .

[11]  Juergen F Kolb,et al.  Nanosecond pulsed electric field generators for the study of subcellular effects , 2006, Bioelectromagnetics.

[12]  Alan E Mark,et al.  Simulation studies of pore and domain formation in a phospholipid monolayer. , 2005, The Journal of chemical physics.

[13]  L. Mir,et al.  [Electrochemotherapy, a new antitumor treatment: first clinical trial]. , 1991, Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie.

[14]  E. Neumann,et al.  Gene transfer into mouse lyoma cells by electroporation in high electric fields. , 1982, The EMBO journal.

[15]  A. Mark,et al.  Simulation of the spontaneous aggregation of phospholipids into bilayers. , 2001, Journal of the American Chemical Society.

[16]  R. Cole,et al.  Evaluation of dielectric behavior by time domain spectroscopy. II. Complex permittivity , 1975 .

[17]  K. Schoenbach,et al.  Self-consistent simulations of electroporation dynamics in biological cells subjected to ultrashort electrical pulses. , 2001, Physical review. E, Statistical, nonlinear, and soft matter physics.

[18]  J. Weaver,et al.  Electroporation of mammalian skin: a mechanism to enhance transdermal drug delivery. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[19]  K. Schoenbach,et al.  High electrical field effects on cell membranes. , 2007, Bioelectrochemistry.

[20]  Siewert J Marrink,et al.  Molecular dynamics simulation of the spontaneous formation of a small DPPC vesicle in water in atomistic detail. , 2004, Journal of the American Chemical Society.