Detection of Salmonella enterica Serovar Rissen in Slaughter Pigs in Northern Ireland

Salmonella enterica serovar Rissen has been recognised as a common serovar in humans and pigs around the world. This study investigated S. Rissen prevalence in pigs slaughtered in Northern Ireland additionally looking at antibiotic susceptibility profiles, genetic profiles and plasmid profiles to provide information on an emerging non-typhoid Salmonella serotype with the potential to cause disease in humans. S. Rissen were isolated on five separate sampling occasions from both the boning hall and slaughter line of a randomly selected single pig abattoir in Northern Ireland (NI). Following antibiotic susceptibility testing against 16 antibiotics, all S. Rissen isolates were identified as susceptible to 15 antibiotics but resistant to tetracycline (R-type: Te). Of the 29 S. Rissen, 27 were isolated from pigs originating in NI and two S. Rissen were isolated from pigs originating in the Republic of Ireland (RoI). The combined results of the PFGE and plasmid profiling analyses were capable of subdividing the S. Rissen isolates into three distinct groups. The data suggests that S. Rissen is an emerging serovar in Northern Ireland and continued surveillance of this serovar is warranted as it has the potential to cause disease in humans.

[1]  P. Patchanee,et al.  Comparative Phenotypic and Genotypic Characterization of Salmonella spp. in Pig Farms and Slaughterhouses in Two Provinces in Northern Thailand , 2015, PloS one.

[2]  L. Peixe,et al.  Clinically relevant multidrug resistant Salmonella enterica in swine and meat handlers at the abattoir. , 2014, Veterinary microbiology.

[3]  H. Argüello,et al.  Prevalence and serovars of Salmonella enterica on pig carcasses, slaughtered pigs and the environment of four Spanish slaughterhouses , 2012 .

[4]  S. Shackelford,et al.  Prevalence, Enumeration, Serotypes, and Antimicrobial Resistance Phenotypes of Salmonella enterica Isolates from Carcasses at Two Large United States Pork Processing Plants , 2012, Applied and Environmental Microbiology.

[5]  A. V. van Hoek,et al.  A quantitative approach towards a better understanding of the dynamics of Salmonella spp. in a pork slaughter-line. , 2012, International journal of food microbiology.

[6]  F. Piras,et al.  Investigation of Salmonella enterica in Sardinian slaughter pigs: prevalence, serotype and genotype characterization. , 2011, International journal of food microbiology.

[7]  R. Porter,et al.  Detection of high serological prevalence and comparison of different tests for Salmonella in pigs in Northern Ireland , 2011, Veterinary Record.

[8]  J. Dewulf,et al.  Detection and characterization of Salmonella in lairage, on pig carcasses and intestines in five slaughterhouses. , 2011, International journal of food microbiology.

[9]  R. Davies,et al.  Longitudinal survey of the occurrence of Salmonella in pigs and the environment of nucleus breeder and multiplier pig herds in England , 2009, Veterinary Record.

[10]  A. Carvajal,et al.  Herd-level risk factors for faecal shedding of Salmonella enterica in Spanish fattening pigs. , 2009, Preventive veterinary medicine.

[11]  F. Aarestrup,et al.  Antimicrobial resistance and molecular epidemiology of Salmonella Rissen from animals, food products, and patients in Thailand and Denmark. , 2008, Foodborne pathogens and disease.

[12]  A. Carvajal,et al.  Antimicrobial Resistance of Salmonella enterica Isolates from Apparently Healthy and Clinically Ill Finishing Pigs in Spain , 2008, Zoonoses and public health.

[13]  A. Vidal,et al.  Salmonella enterica Infections in Spanish Swine Fattening Units , 2007, Zoonoses and public health.

[14]  R. Astorga,et al.  Surveillance and antimicrobial resistance of Salmonella strains isolated from slaughtered pigs in Spain. , 2007, Journal of food protection.

[15]  J. Casal,et al.  Epidemiology of salmonella infections in pig units and antimicrobial susceptibility profiles of the strains of Salmonella species isolated , 2006, Veterinary Record.

[16]  Madalena Vieira-Pinto,et al.  Unveiling contamination sources and dissemination routes of Salmonella sp. in pigs at a Portuguese slaughterhouse through macrorestriction profiling by pulsed-field gel electrophoresis. , 2006, International journal of food microbiology.

[17]  B. Swaminathan,et al.  Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. , 2006, Foodborne pathogens and disease.

[18]  L. Herman,et al.  Phenotypic and Molecular Typing of Salmonella Strains Reveals Different Contamination Sources in Two Commercial Pig Slaughterhouses , 2004, Applied and Environmental Microbiology.

[19]  D H Persing,et al.  Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing , 1995, Journal of clinical microbiology.

[20]  S. T. Liu,et al.  Rapid procedure for detection and isolation of large and small plasmids , 1981, Journal of bacteriology.

[21]  Efsa Journal,et al.  European Food Safety Authority, European Centre for Disease Prevention and Control; The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 2009 , 2011 .