Characterization of Cell Response on Patterned Stiffness Substrate by AFAM

The stiffness of extracellular matrix strongly influences cells behavior, but characterization of substrate stiffness patterns is a challenge. In our study, atomic force acoustic microscopy (AFAM) was used to study the cell responses on substrate stiffness patterns. The nano-stripe stiffness pattern was imprinted on SU-8 film. Our results show that AFAM acoustic images can detect both the cell surfaces and the stiffness pattern on the SU-8 substrate. We find that the cells significant deformation on the patterned stiffness substrate. Moreover, when using cytochalasin-D to interfere actin filaments, there is a significantly decrease in cell area for cells seeded on the patterned stiffness substrate. This study provides new information for the design and characterization of extracellular matrix interfaces, and contributes to the understanding of cell-substate interactions.

[1]  Zuobin Wang,et al.  Single-cell patterning regulation by physically modified silicon nanostructures. , 2022, Analytical methods : advancing methods and applications.

[2]  Jinju Chen,et al.  Simultaneous Measurement of Single-Cell Mechanics and Cell-to-Materials Adhesion Using Fluidic Force Microscopy. , 2022, Langmuir : the ACS journal of surfaces and colloids.

[3]  Yuhang Chen,et al.  Measurement of Film-Elastomer Interface Adhesion by Continuous Buckling. , 2021, ACS applied materials & interfaces.

[4]  J. Toca-Herrera,et al.  Substrate stiffness modulates the viscoelastic properties of MCF-7 cells. , 2021, Journal of the mechanical behavior of biomedical materials.

[5]  Ying Wang,et al.  Cell spreading behaviors on hybrid nanopillar and nanohole arrays , 2021, Nanotechnology.

[6]  J. Nakanishi,et al.  Viscoelastically tunable substrates elucidate the interface-relaxation-dependent adhesion and assembly behaviors of epithelial cells. , 2021, Biomaterials.

[7]  Zuobin Wang,et al.  Durotaxis behavior of bEnd.3 cells on soft substrate with patterned platinum nanoparticle array , 2020, Applied Nanoscience.

[8]  Liguo Tian,et al.  Investigating effects of silicon nanowire and nanohole arrays on fibroblasts via AFAM , 2020, Applied Nanoscience.

[9]  Yuhang Chen,et al.  Subsurface imaging of rigid particles buried in a polymer matrix based on atomic force microscopy mechanical sensing. , 2019, Ultramicroscopy.

[10]  Ying Wang,et al.  Atomic force acoustic microscopy reveals the influence of substrate stiffness and topography on cell behavior , 2019, Beilstein journal of nanotechnology.

[11]  P. Janmey,et al.  Stiffness Sensing in Cells and Tissues. , 2019, Physiological reviews.

[12]  Mingyue Ding,et al.  Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM) , 2019, Cells.

[13]  Han-Sung Jung,et al.  Artificial cellular nano-environment composed of collagen-based nanofilm promotes osteogenic differentiation of mesenchymal stem cells. , 2019, Acta biomaterialia.

[14]  H. Kim,et al.  Effect of Topographical Feature Size on the Trend of Cell Behaviors , 2018, IEEE Transactions on Nanotechnology.

[15]  S. Dudek,et al.  Development of ultrasound bioprobe for biological imaging , 2017, Science Advances.

[16]  A. Corvin,et al.  Characterization of SH-SY5Y human neuroblastoma cell growth over glass and SU-8 substrates. , 2017, Journal of biomedical materials research. Part A.

[17]  Zuobin Wang,et al.  Response of MG63 osteoblast cells to surface modification of Ti-6Al-4V implant alloy by laser interference lithography , 2017 .

[18]  Anish Kumar,et al.  Elasticity mapping of precipitates in nickel-base superalloys using atomic force acoustic microscopy , 2016, Journal of Materials Science.

[19]  J. Burdick,et al.  Hydrogels with differential and patterned mechanics to study stiffness-mediated myofibroblastic differentiation of hepatic stellate cells. , 2014, Journal of the mechanical behavior of biomedical materials.

[20]  D. Nisbet,et al.  Specific control of cell–material interactions: Targeting cell receptors using ligand-functionalized polymer substrates , 2014 .

[21]  Barjor Gimi,et al.  In vitro and in vivo evaluation of SU-8 biocompatibility. , 2013, Materials science & engineering. C, Materials for biological applications.

[22]  D. Weihs,et al.  Intracellular Mechanics and Activity of Breast Cancer Cells Correlate with Metastatic Potential , 2012, Cell Biochemistry and Biophysics.

[23]  F. Grinnell,et al.  Microtubule function in fibroblast spreading is modulated according to the tension state of cell–matrix interactions , 2007, Proceedings of the National Academy of Sciences.

[24]  Amelio,et al.  Quantitative determination of contact stiffness using atomic force acoustic microscopy , 2000, Ultrasonics.

[25]  Ute Rabe,et al.  Acoustic microscopy by atomic force microscopy , 1994 .