Nanocapsule induced morphology and migration changes in single cell layers quantified with digital holographic microscopy

We applied quantitative phase imaging (QPI) for quantification of nanocapsule-induced morphology and migration changes in single cell layers. In time-lapse observations, cells were monitored with a Mach-Zehnder interferometerbased off-axis digital holographic microscopy (DHM) setup. For quantification of cell migration, single cells were tracked in the recorded series of quantitative DHM phase images. Moreover, QPI images were evaluated as novel stainfree assay to quantify the temporal course of global cellular morphology changes. The label-free acquired data shows that capsaicin-loaded and unloaded chitosan nanocapsules, and also free capsaicin, significantly influence the direction of cell migration and cellular motility.

[1]  Gabriel Popescu,et al.  Fourier phase microscopy for investigation of biological structures and dynamics. , 2004, Optics letters.

[2]  D. Dirksen,et al.  Autofocusing in digital holographic phase contrast microscopy on pure phase objects for live cell imaging. , 2008, Applied optics.

[3]  Huafeng Ding,et al.  Instantaneous Spatial Light Interference Microscopy. , 2010, Optics express.

[4]  C. Fang-Yen,et al.  Tomographic phase microscopy , 2008, Nature Methods.

[5]  Pinhas Girshovitz,et al.  Generalized cell morphological parameters based on interferometric phase microscopy and their application to cell life cycle characterization , 2012, Biomedical optics express.

[6]  Patrik Langehanenberg,et al.  Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy. , 2010, Journal of biomedical optics.

[7]  B. Wattellier,et al.  Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells. , 2009, Optics express.

[8]  O. Haeberlé,et al.  High-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples. , 2009, Optics letters.

[9]  Patrik Langehanenberg,et al.  Differential cytotoxic actions of Shiga toxin 1 and Shiga toxin 2 on microvascular and macrovascular endothelial cells , 2010, Thrombosis and Haemostasis.

[10]  Daniel Carl,et al.  Investigation of living pancreas tumor cells by digital holographic microscopy. , 2006, Journal of biomedical optics.

[11]  B. Kemper,et al.  Digital holographic microscopy for live cell applications and technical inspection. , 2008, Applied optics.

[12]  C. Gorzelanny,et al.  Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells , 2015, Scientific Reports.

[13]  B. Kemper,et al.  Survivin, a target to modulate the radiosensitivity of Ewing’s sarcoma , 2012, Strahlentherapie und Onkologie.

[14]  Universitätsklinikum Münster,et al.  Survivin, a target to modulate the radiosensitivity of Ewing's sarcoma , 2012 .

[15]  Christian Depeursinge,et al.  Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy. , 2009, Journal of biomedical optics.

[16]  Steffi Ketelhut,et al.  Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing. , 2016, Journal of visualized experiments : JoVE.

[17]  Baoli Yao,et al.  Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy , 2017, BiOS.

[18]  E. Cuche,et al.  Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. , 2005, Optics letters.

[19]  E. Cuche,et al.  Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. , 1999, Applied optics.

[20]  Steffi Ketelhut,et al.  Nanoencapsulated capsaicin changes migration behavior and morphology of madin darby canine kidney cell monolayers , 2017, PloS one.

[21]  Steffi Ketelhut,et al.  Enhanced quantitative phase imaging in self-interference digital holographic microscopy using an electrically focus tunable lens. , 2014, Biomedical optics express.

[22]  Chun-Min Lo,et al.  High-resolution quantitative phase-contrast microscopy by digital holography. , 2005, Optics express.

[23]  Natan T Shaked,et al.  Reflective interferometric chamber for quantitative phase imaging of biological sample dynamics. , 2010, Journal of biomedical optics.

[24]  Steffi Ketelhut,et al.  New approaches for the analysis of confluent cell layers with quantitative phase digital holographic microscopy , 2016, SPIE BiOS.

[25]  Daniel Carl,et al.  Modular digital holographic microscopy system for marker free quantitative phase contrast imaging of living cells , 2006, SPIE Photonics Europe.

[26]  Daniel Carl,et al.  Parameter-optimized digital holographic microscope for high-resolution living-cell analysis. , 2004, Applied optics.

[27]  Jong Chul Ye,et al.  Self-reference quantitative phase microscopy for microfluidic devices. , 2010, Optics letters.

[28]  R. Dasari,et al.  Diffraction phase microscopy for quantifying cell structure and dynamics. , 2006, Optics letters.

[29]  Steffi Ketelhut,et al.  Quantitative Stain-Free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy , 2014, PloS one.

[30]  Steffi Ketelhut,et al.  Multimodal label-free in vitro toxicity testing with digital holographic microscopy , 2014, Photonics Europe.

[31]  Gabriel Popescu,et al.  Hilbert phase microscopy for investigating fast dynamics in transparent systems. , 2005, Optics letters.

[32]  Gabriel Popescu,et al.  Optical imaging of cell mass and growth dynamics. , 2008, American journal of physiology. Cell physiology.

[33]  Natan T Shaked,et al.  Dual-interference-channel quantitative-phase microscopy of live cell dynamics. , 2009, Optics letters.

[34]  Jérôme Parent,et al.  Label-free cytotoxicity screening assay by digital holographic microscopy. , 2013, Assay and drug development technologies.

[35]  Tomasz Kozacki,et al.  Reconstruction of refractive-index distribution in off-axis digital holography optical diffraction tomographic system. , 2009, Optics express.