Trypan Blue Exclusion Test of Cell Viability

The protocol described in this appendix allows for light microscopic quantitation of cell viability. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cells). © 2015 by John Wiley & Sons, Inc.

[1]  C. Chung,et al.  Comparison of the automated fluorescence microscopic viability test with the conventional and flow cytometry methods , 2011, Journal of clinical laboratory analysis.

[2]  W. Strober,et al.  Monitoring cell growth. , 2001, Current protocols in immunology.

[3]  O. Martins-Filho,et al.  Trypan blue exclusion assay by flow cytometry , 2014, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[4]  G. Otten,et al.  Flow cytometry analysis using the Becton Dickinson FACS Calibur. , 2002, Current protocols in immunology.