Production of Protease Enzyme from Bacillus Clausii Sm 3

Soil was collected and serially diluted using sterile distilled water. Totally 5 bacterial colonies are isolated, all the 5 strains showed clear zone in protease plate assay, particularly the strain SM3 exhibited clear zone around the colony on skim agar plates. On amylase assay the activity was found to be 0.99 U/min/ml. Considering the macroscopic, microscopic, biochemical and physiological characteristics the strain was identified as Bacillus clausiii SM3. It was seen that protease produced by Bacillus clausii SM3 had high capability of removing the blood stain, which indicates its potential in detergent industries. From this study it was came to be know that the study organism (Bacillus clausiii SM3) isolated from soil can be used as an effective source for the production of protease enzyme.

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