Hyperspectral optical diffraction tomography.

Here, we present a novel microscopic technique for measuring wavelength-dependent three-dimensional (3-D) distributions of the refractive indices (RIs) of microscopic samples in the visible wavelengths. Employing 3-D quantitative phase microscopy techniques with a wavelength-swept source, 3-D RI tomograms were obtained in the range of 450 - 700 nm with a spectral resolution of a few nanometers. The capability of the technique was demonstrated by measuring the hyperspectral 3-D RI tomograms of polystyrene beads, human red blood cells, and hepatocytes. The results demonstrate the potential for label-free molecular specific 3-D tomography of biological samples.

[1]  Gabriel Popescu,et al.  Diffraction phase and fluorescence microscopy. , 2006, Optics express.

[2]  V. Tuchin,et al.  The refractive index of human hemoglobin in the visible range , 2011, Physics in medicine and biology.

[3]  K. Tam,et al.  Tomographical imaging with limited-angle input , 1981 .

[4]  W. R. Wiley,et al.  Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering , 1999 .

[5]  R. Dasari,et al.  Diffraction phase microscopy for quantifying cell structure and dynamics. , 2006, Optics letters.

[6]  YongKeun Park,et al.  Biomedical applications of holographic microspectroscopy [invited]. , 2014, Applied optics.

[7]  Chulhee Choi,et al.  Hepatitis C virus infection enhances TNFα‐induced cell death via suppression of NF‐κB , 2012, Hepatology.

[8]  YongKeun Park,et al.  Real-time quantitative phase imaging with a spatial phase-shifting algorithm. , 2011, Optics letters.

[9]  S. Wold,et al.  Principal component analysis of multivariate images , 1989 .

[10]  YongKeun Park,et al.  Three-dimensional refractive index tomograms and deformability of individual human red blood cells from cord blood of newborn infants and maternal blood , 2015, Journal of biomedical optics.

[11]  P. So,et al.  Diffraction optical tomography using a quantitative phase imaging unit. , 2014, Optics letters.

[12]  Mor Habaza,et al.  Tomographic phase microscopy with 180° rotation of live cells in suspension by holographic optical tweezers. , 2015, Optics letters.

[13]  Francisco E. Robles,et al.  Molecular imaging true-colour spectroscopic optical coherence tomography. , 2011, Nature photonics.

[14]  J. Mansfield,et al.  Multispectral imaging in biology and medicine: Slices of life , 2006, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[15]  D. Brasaemle,et al.  Fixation Methods for the Study of Lipid Droplets by Immunofluorescence Microscopy , 2003, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[16]  C. Fang-Yen,et al.  Optical diffraction tomography for high resolution live cell imaging. , 2009, Optics express.

[17]  J. Paul Robinson,et al.  Loss of image quality in photobleaching during microscopic imaging of fluorescent probes bound to chromatin. , 2005, Journal of biomedical optics.

[18]  Yizheng Zhu,et al.  Quantitative phase spectroscopy , 2012, Biomedical optics express.

[19]  P. Marquet,et al.  Marker-free phase nanoscopy , 2013, Nature Photonics.

[20]  YongKeun Park,et al.  High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography , 2013, Journal of biomedical optics.

[21]  Kyoohyun Kim,et al.  High-Resolution 3-D Refractive Index Tomography and 2-D Synthetic Aperture Imaging of Live Phytoplankton , 2014 .

[22]  Björn Kemper,et al.  Tomographic phase microscopy of living three-dimensional cell cultures , 2014, Journal of biomedical optics.

[23]  Kyoohyun Kim,et al.  Label-free characterization of white blood cells by measuring 3D refractive index maps. , 2015, Biomedical optics express.

[24]  Zahid Yaqoob,et al.  Single-shot quantitative dispersion phase microscopy. , 2012, Applied physics letters.

[25]  Sung-Hee Hong,et al.  Characterizations of individual mouse red blood cells parasitized by Babesia microti using 3-D holographic microscopy , 2015, Scientific Reports.

[26]  Jaeduck Jang,et al.  Spectro-refractometry of individual microscopic objects using swept-source quantitative phase imaging. , 2013, Analytical chemistry.

[27]  YoungJu Jo,et al.  Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications , 2013, Sensors.

[28]  Jaeduck Jang,et al.  Dynamic spectroscopic phase microscopy for quantifying hemoglobin concentration and dynamic membrane fluctuation in red blood cells. , 2012, Optics express.

[29]  Jong Chul Ye,et al.  Real-time Visualization of 3-d Dynamic Microscopic Objects Using Optical Diffraction Tomography References and Links , 2022 .

[30]  G. Popescu.  Quantitative Phase Imaging of Cells and Tissues , 2011 .

[31]  Jong Chul Ye,et al.  Comparative study of iterative reconstruction algorithms for missing cone problems in optical diffraction tomography. , 2015, Optics express.

[32]  Ivan D. Nikolov,et al.  Dispersion Properties of Optical Polymers , 2009 .

[33]  YongKeun Park,et al.  Profiling individual human red blood cells using common-path diffraction optical tomography , 2014, Scientific Reports.

[34]  Youngchan Kim,et al.  Common-path diffraction optical tomography for investigation of three-dimensional structures and dynamics of biological cells. , 2014, Optics express.

[35]  Ram Dixit,et al.  Cell damage and reactive oxygen species production induced by fluorescence microscopy: effect on mitosis and guidelines for non-invasive fluorescence microscopy. , 2003, The Plant journal : for cell and molecular biology.

[36]  A. Devaney.  Inverse-scattering theory within the Rytov approximation. , 1981, Optics letters.

[37]  H. Pham,et al.  Spectroscopic diffraction phase microscopy. , 2012, Optics letters.

[38]  Yongjin Sung,et al.  Quantitative dispersion microscopy , 2010, Biomedical optics express.

[39]  V. Lauer.  New approach to optical diffraction tomography yielding a vector equation of diffraction tomography and a novel tomographic microscope , 2002, Journal of microscopy.

[40]  Ton G van Leeuwen,et al.  Oxygen saturation-dependent absorption and scattering of blood. , 2004, Physical review letters.

[41]  M. Friebel,et al.  Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250-1100 nm dependent on concentration. , 2006, Applied optics.

[42]  Francisco E. Robles,et al.  Nonlinear phase dispersion spectroscopy. , 2011, Optics letters.

[43]  YongKeun Park,et al.  Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells , 2009, BiOS.

[44]  Ivan D. Nikolov,et al.  Measuring the refractometric characteristics of optical plastics , 2003 .