PELDOR spectroscopy distance fingerprinting of the octameric outer-membrane protein Wza from Escherichia coli.

Distance fingerprinting: Pulsed electron-electron double resonance spectroscopy (PELDOR) is applied to the octameric membrane protein complex Wza of E. coli. The data yielded a detailed distance fingerprint of its periplasmic region that compares favorably to the crystal structure. These results provide the foundation to study conformation changes from interaction with partner proteins.

[1]  C. Whitfield,et al.  Periplasmic export machines for outer membrane assembly. , 2008, Current opinion in structural biology.

[2]  T. Prisner,et al.  Counting the monomers in nanometer-sized oligomers by pulsed electron-electron double resonance. , 2007, Journal of the American Chemical Society.

[3]  T. Prisner,et al.  Long-range distance determinations in biomacromolecules by EPR spectroscopy , 2007, Quarterly Reviews of Biophysics.

[4]  E. Padan,et al.  High-resolution structure of a Na+/H+ antiporter dimer obtained by pulsed electron paramagnetic resonance distance measurements. , 2007, Biophysical journal.

[5]  Richard A Stein,et al.  Dipolar coupling between nitroxide spin labels: the development and application of a tether-in-a-cone model. , 2006, Biophysical journal.

[6]  K. J. Oh,et al.  Crystal structures of spin labeled T4 lysozyme mutants: implications for the interpretation of EPR spectra in terms of structure. , 2000, Biochemistry.

[7]  Gunnar Jeschke,et al.  Determination of the nanostructure of polymer materials by electron paramagnetic resonance spectroscopy , 2002 .

[8]  A. Milov,et al.  Pulsed electron double resonance (PELDOR) and its applications in free-radicals research , 1998 .

[9]  A. Milov,et al.  Electron-electron double resonance in electron spin echo: Model biradical systems and the sensitized photolysis of decalin , 1984 .

[10]  C. Whitfield.  Biosynthesis and assembly of capsular polysaccharides in Escherichia coli. , 2006, Annual review of biochemistry.

[11]  H. Steinhoff,et al.  A comparative electron paramagnetic resonance study of the nucleotide-binding domains' catalytic cycle in the assembled maltose ATP-binding cassette importer. , 2008, Biophysical journal.

[12]  Duilio Cascio,et al.  Structural determinants of nitroxide motion in spin‐labeled proteins: Solvent‐exposed sites in helix B of T4 lysozyme , 2008, Protein science : a publication of the Protein Society.

[13]  James H. Naismith,et al.  Wza the translocon for E. coli capsular polysaccharides defines a new class of membrane protein , 2006, Nature.