The multi-protein family of Arabidopsis sulphotransferases and their relatives in other plant species.

All members of the sulphotransferase (SOT, EC 2.8.2.-) protein family use 3'-phosphoadenosine 5'-phosphosulphate (PAPS) as the sulphuryl donor and transfer the sulphonate group to an appropriate hydroxyl group of several classes of substrates. These enzymes have highly conserved domains and can be found in eubacteria and eukaryotes. In mammals, sulphate conjugation catalysed by SOTs constitutes an important reaction in the transformation of xenobiotics, and in the modulation of the biological activity of steroid hormones and neurotransmitters. In plants, sulphate-conjugation reactions seem to play an important role in plant growth, development, and adaptation to stress. To date only a few plant SOTs have been characterized in detail. The flavonol 3- and 4'-SOTs from Flaveria species (Asteraceae), which catalyse the sulphonation of flavonol aglycones and flavonol 3-sulphates, respectively, were the first plant SOTs for which cDNA clones were isolated. The plasma membrane associated gallic acid SOT of Mimosa pudica L. pulvini cells may be intrinsic to signalling events that modify the seismonastic response. In Brassica napus L. a SOT catalyses the O-sulphonation of brassinosteroids and thereby abolishes specifically the biological activity of 24-epibrassinolide. The fully sequenced genome of Arabidopsis thaliana Heynh. contains in total 18 genes that are likely to encode SOT proteins based on sequence similarities of the translated products with an average identity of 51.1%. So far only one SOT from A. thaliana (At5g07000) was functionally characterized: the protein was shown to catalyse the sulphonation of 12-hydroxyjasmonate and thereby inactivate excess jasmonic acid in plants. The substrates and, therefore, the physiological roles of SOTs are very diverse. By using the numerous informative databases and methods available for the model plant A. thaliana, the elucidation of the functional role of the SOT protein family will be accelerated.

[1]  L. Pedersen,et al.  Crystal structure‐based studies of cytosolic sulfotransferase , 2001, Journal of biochemical and molecular toxicology.

[2]  Harry L. T. Mobley,et al.  Physiology and Molecular Biology , 2001 .

[3]  R. Kato,et al.  Structural similarity and diversity of sulfotransferases. , 1994, Chemico-biological interactions.

[4]  L. Pedersen,et al.  Structure and function of sulfotransferases. , 2001, Archives of biochemistry and biophysics.

[5]  Chau‐Ching Liu,et al.  Sulfation of hydroxychlorobiphenyls. Molecular cloning, expression, and functional characterization of zebrafish SULT1 sulfotransferases. , 2003, European Journal of Biochemistry.

[6]  G. Adam,et al.  Inactivation of Brassinosteroid Biological Activity by a Salicylate-inducible Steroid Sulfotransferase from Brassica napus * , 1999, The Journal of Biological Chemistry.

[7]  J. Ebinger.  Asparagus officinalis L. , 1969 .

[8]  K. Nakamura,et al.  Diversity of Arabidopsis genes encoding precursors for phytosulfokine, a peptide growth factor. , 2001, Plant physiology.

[9]  F. Marsolais,et al.  Recent developments in the study of the structure-function relationship of flavonol sulfotransferases. , 1998, Chemico-biological interactions.

[10]  E. Kempner,et al.  Purification of the Golgi adenosine 3'-phosphate 5'-phosphosulfate transporter, a homodimer within the membrane. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[11]  P. Lerouge,et al.  Sulphated lipo-oligosaccharide signals of Rhizobium meliloti elicit root nodule organogenesis in alfalfa , 1991, Nature.

[12]  C. Lillig,et al.  Molecular and catalytic properties of Arabidopsis thaliana adenylyl sulfate (APS)-kinase. , 2001, Archives of biochemistry and biophysics.

[13]  J. Lafontaine,et al.  The enzyme involved in sulfation of the turgorin, gallic acid 4-O-(beta-D-glucopyranosyl-6'-sulfate) is pulvini-localized in Mimosa pudica. , 1997, The Plant journal : for cell and molecular biology.

[14]  Jutta Papenbrock,et al.  Identification and characterization of single‐domain thiosulfate sulfurtransferases from Arabidopsis thaliana , 2002, FEBS letters.

[15]  K. Nakamura,et al.  Oryza sativa PSK gene encodes a precursor of phytosulfokine-alpha, a sulfated peptide growth factor found in plants. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[16]  J. Poulton,et al.  Partial Purification and Characterization of a 3'- Phosphoadenosine 5' -Phosphosulfate: Desulfoglucosinolate Sulfotransferase from Cress (Lepidium sativum). , 1990, Plant physiology.

[17]  G. Tennekoon,et al.  Reconstitution of adenosine 3'-phosphate 5'-phosphosulfate transporter from rat brain. , 1988, Biochemical and biophysical research communications.

[18]  J. Capasso,et al.  Effect of atractylosides, palmitoyl coenzyme A, and anion transport inhibitors on translocation of nucleotide sugars and nucleotide sulfate into Golgi vesicles. , 1984, The Journal of biological chemistry.

[19]  C. Wasternack,et al.  Biochemical and Molecular Characterization of a Hydroxyjasmonate Sulfotransferase from Arabidopsis thaliana * , 2003, The Journal of Biological Chemistry.

[20]  I. J. Faulkner,et al.  Flavonoids and flavonoid sulphates as probes of auxin-transport regulation in Cucurbita pepo hypocotyl segments and vesicles , 1992, Planta.

[21]  D. Scheel,et al.  Physiology and Molecular Biology of Phenylpropanoid Metabolism , 1989 .

[22]  J. Papenbrock,et al.  Intracellular Localization of Arabidopsis Sulfurtransferases1 , 2004, Plant Physiology.

[23]  O. Bagasra,et al.  Proceedings of the National Academy of Sciences , 1914, Science.

[24]  B. Halkier,et al.  Biosynthesis and metabolic engineering of glucosinolates , 2002, Amino Acids.

[25]  W. Huttner,et al.  Tyrosine-O-sulfated proteins of PC12 pheochromocytoma cells and their sulfation by a tyrosylprotein sulfotransferase. , 1983, The Journal of biological chemistry.

[26]  N. Brisson,et al.  Chimeric Flavonol Sulfotransferases Define a Domain Responsible for Substrate and Position Specificities (*) , 1995, The Journal of Biological Chemistry.

[27]  The Arabidopsis Genome Initiative.  Analysis of the genome sequence of the flowering plant Arabidopsis thaliana , 2000, Nature.

[28]  N. Taniguchi,et al.  Sulfotransferases and sulfated oligosaccharides , 2002, Medicinal research reviews.

[29]  F. Marsolais,et al.  Biochemistry and molecular biology of plant sulfotransferases , 1997, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[30]  P. von Ballmoos,et al.  Adenosine 5′-Phosphosulfate Sulfotransferase and Adenosine 5′-Phosphosulfate Reductase Are Identical Enzymes* , 2000, The Journal of Biological Chemistry.

[31]  R. Weinshilboum,et al.  Sulfotransferase molecular biology: cDNAs and genes , 1997, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[32]  C. Niehrs,et al.  Protein tyrosine sulfation, 1993--an update. , 1994, Chemico-biological interactions.

[33]  R. Ibrahim,et al.  Partial Purification and Characterization of Three Flavonol-Specific Sulfotransferases from Flaveria chloraefolia. , 1989, Plant physiology.

[34]  A. Superti-Furga.  A defect in the metabolic activation of sulfate in a patient with achondrogenesis type IB. , 1994, American journal of human genetics.

[35]  O Habuchi,et al.  Diversity and functions of glycosaminoglycan sulfotransferases. , 2000, Biochimica et biophysica acta.

[36]  F. Marsolais,et al.  Identification of Amino Acid Residues Critical for Catalysis and Cosubstrate Binding in the Flavonol 3-Sulfotransferase (*) , 1995, The Journal of Biological Chemistry.

[37]  M. Kanehisa,et al.  A knowledge base for predicting protein localization sites in eukaryotic cells , 1992, Genomics.

[38]  R. Mendel,et al.  Fluorescent proteins in poplar: a useful tool to study promoter function and protein localization. , 2004, Plant biology.

[39]  A. Hanson,et al.  Choline-O-Sulfate Biosynthesis in Plants (Identification and Partial Characterization of a Salinity-Inducible Choline Sulfotransferase from Species of Limonium (Plumbaginaceae) , 1994, Plant physiology.

[40]  T. Leustek,et al.  Differential subcellular localization and expression of ATP sulfurylase and 5'-adenylylsulfate reductase during ontogenesis of Arabidopsis leaves indicates that cytosolic and plastid forms of ATP sulfurylase may have specialized functions. , 2000, Plant physiology.

[41]  Heping Yang,et al.  Existence of a plant tyrosylprotein sulfotransferase: novel plant enzyme catalyzing tyrosine O‐sulfation of preprophytosulfokine variants in vitro , 2000, FEBS letters.

[42]  F. Marsolais,et al.  Mutational analysis of domain II of flavonol 3-sulfotransferase. , 1997, European journal of biochemistry.

[43]  C. Klaassen,et al.  The importance of 3‘‐phosphoadenosine 5‘‐phosphosulfate (PAPS) in the regulation of sulfation , 1997, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[44]  R. Mithen.  Glucosinolates – biochemistry, genetics and biological activity , 2001, Plant Growth Regulation.

[45]  H. Koshino,et al.  Structure of a Tuber-inducing Stimulus from Potato Leaves (Solanum tuberosum L.) , 1989 .

[46]  D. Roby,et al.  Molecular cloning of a sulfotransferase in Arabidopsis thaliana and regulation during development and in response to infection with pathogenic bacteria , 1996, Plant Molecular Biology.

[47]  S. Gidda,et al.  Chapter Fourteen Plant soluble sulfotransferases: Structural and functional similarity with mammalian enzymes , 2000 .

[48]  N. Brisson,et al.  Molecular characterization of two plant flavonol sulfotransferases. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[49]  S. Brunak,et al.  Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. , 2000, Journal of molecular biology.

[50]  J. Tokuhisa,et al.  Benzoic acid glucosinolate esters and other glucosinolates from Arabidopsis thaliana. , 2002, Phytochemistry.