Effect of dietary Lippia citriodora extract on reproductive and productive performance and plasma biochemical parameters in rabbit does

Two doses of natural extract from Lippia citriodora (titrated in verbascoside) were assessed in New Zealand White rabbit does evaluating selected reproductive, productive and plasma biochemical parameters. After 1 week of adaptation period, the trial on 45 rabbit does for three consecutive reproductive cycles was conducted; does were divided into three groups of 15 animals each, homogenous by age (1 year ± 2 weeks), bodyweight (4.77 ± 0.21 kg) and parity (2 ± 1). A control group (CON) did not receive the dietary supplement in the feed and the other two groups received 1 g of natural extract supplement in the feed (5 mg verbascoside/kg feed; LNE) and 2 g of natural extract (10 mg verbascoside/kg feed; HNE). The use of a dietary NE supplement improved kit bodyweight at weaning (934 vs 1104 g; P < 0.001), and average daily weight gain from birth to weaning (24.7 vs 29.7 g/day; P < 0.001), with no NE dose effect. In the LNE and HNE groups serum triglycerides, total cholesterol, low density lipoprotein cholesterol, bilirubin, and activities of alkaline phosphatase, alanine aminotransferase (P < 0.05) and aspartate aminotransferase (P < 0.01) decreased and high density lipoprotein cholesterol (P < 0.01) increased, according to the cycle effect. The dietary supplement also improved blood oxidative status markers in the experimental groups due to an increase in the concentrations of plasma vitamin A and E (P < 0.01) and a decrease in plasma reactive oxygen metabolites and thiobarbituric acid reactive substances values (P < 0.01). In conclusion, the dietary Lippia NE supplement improved selected productive and reproductive parameters and the animal welfare of does, expressed by a general improvement of blood profile, with no effect of the dose.

[1]  M. Palazzo,et al.  Effect of dietary phenylpropanoid glycoside-based natural extracts on blood parameters and productive performance in intensively-reared young hares , 2018 .

[2]  R. Rossi,et al.  Influence of Lippia citriodora verbascoside on growth performance, antioxidant status, and serum immunoglobulins content in piglets. , 2018 .

[3]  C. Costagliola,et al.  Effects of Verbascoside-Based Diet on Blood and Plasma Constituents of Rabbits , 2013, Journal of the American College of Nutrition.

[4]  M. Palazzo,et al.  The effects of lemon verbena (Lippia citriodora) verbascoside on the productive performance, plasma oxidative status, and some blood metabolites in suckling lambs , 2013 .

[5]  K. Abdoun,et al.  Effects of dietary seaweed (Ulva lactuca) supplementation on the reproductive performance of buck and doe rabbits , 2013 .

[6]  M. Palazzo,et al.  Effects of verbascoside on plasma oxidative status and blood and milk production parameters during the peripartum period in Lacaune ewes , 2012 .

[7]  M. Palazzo,et al.  Assessment of a natural dietary extract, titrated in phenylpropanoid glycosides, on blood parameters and plasma oxidative status in intensively reared Italian hares (Lepus corsicanus). , 2011, Animal : an international journal of animal bioscience.

[8]  Y. Eid Dietary grape pomace affects lipid peroxidation and antioxidative status in rabbit semen. , 2010 .

[9]  E. Mazzon,et al.  PPAR-α Contributes to the Anti-Inflammatory Activity of Verbascoside in a Model of Inflammatory Bowel Disease in Mice , 2010, PPAR research.

[10]  A. Segura‐Carretero,et al.  Correlation between plasma antioxidant capacity and verbascoside levels in rats after oral administration of lemon verbena extract , 2009 .

[11]  P. Lekeux,et al.  The oxidant/antioxidant equilibrium in horses. , 2008, Veterinary journal.

[12]  F. Farrokhi,et al.  Investigation the Effects of Curcumin on Serum Hepatic Enzymes Activity in a Rheumatoid Arthritis Model , 2008 .

[13]  P. Roach,et al.  A Green Tea Catechin Extract Upregulates the Hepatic Low-Density Lipoprotein Receptor in Rats , 2007, Lipids.

[14]  D. Gatsing,et al.  The Effects of Boswellia dalzielii (Burseraceae) Aqueous Bark Extract on Rat Liver Function , 2007 .

[15]  M. Kerntopf,et al.  Chemical composition, toxicological aspects and antifungal activity of essential oil from Lippia sidoides Cham. , 2007, The Journal of antimicrobial chemotherapy.

[16]  J. Lykkesfeldt,et al.  Oxidants and antioxidants in disease: oxidative stress in farm animals. , 2007, Veterinary journal.

[17]  H. Mo,et al.  Induction of geranyl pyrophosphate pyrophosphatase activity by cholesterol-suppressive isoprenoids , 1995, Lipids.

[18]  N. Kamiloğlu,et al.  Alteration in antioxidant status and lipid peroxidation of sheep previously treated with vitamin A and beta-carotene during breeding and periparturient period , 2006 .

[19]  G. Stradaioli,et al.  Dietary grape poliphenols modulate oxidative stress in ageing rabbits , 2005 .

[20]  B. P. Kota,et al.  Herbal or natural medicines as modulators of peroxisome proliferator-activated receptors and related nuclear receptors for therapy of metabolic syndrome. , 2005, Basic & clinical pharmacology & toxicology.

[21]  V. Fogliano,et al.  Dietary Antioxidant Compounds and Liver Health , 2004, Critical reviews in food science and nutrition.

[22]  S. Passi,et al.  Phenolic constituents and antioxidant activity of Wendita calysina leaves (Burrito), a folk Paraguayan tea. , 2004, Journal of agricultural and food chemistry.

[23]  S. Moochhala,et al.  Simultaneous determination of vitamins C, E and beta-carotene in human plasma by high-performance liquid chromatography with photodiode-array detection. , 2004, Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques.

[24]  G. Norata,et al.  [Peroxisome proliferator activated receptors and cardiovascular disorders]. , 2003, Italian heart journal. Supplement : official journal of the Italian Federation of Cardiology.

[25]  E. Fernandes,et al.  Studies on the antioxidant activity of Lippia citriodora infusion: scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid. , 2002, Biological & pharmaceutical bulletin.

[26]  R. Reiter,et al.  Causes of Oxidative Stress in the Pre- and Perinatal Period , 2002, Neonatology.

[27]  F. Carvalho,et al.  Antioxidant activity of Centaurium erythraea infusion evidenced by its superoxide radical scavenging and xanthine oxidase inhibitory activity. , 2001, Journal of agricultural and food chemistry.

[28]  H. Seguchi,et al.  Evaluation of the process for superoxide production by NADPH oxidase in human neutrophils: evidence for cytoplasmic origin of superoxide , 2001, Redox report : communications in free radical research.

[29]  S. Handa,et al.  Antiinflammatory activity and chemical composition of extracts of Verbena officinalis , 2000, Phytotherapy research : PTR.

[30]  A. Nicolaides,et al.  A simple test to monitor oxidative stress. , 1999, International angiology : a journal of the International Union of Angiology.

[31]  Yu Huang,et al.  Regeneration of α-Tocopherol in Human Low-Density Lipoprotein by Green Tea Catechin , 1999 .

[32]  K. Hase,et al.  Hepatoprotective activity of phenylethanoids from Cistanche deserticola. , 1998, Planta medica.

[33]  H. Esterbauer,et al.  Methods for determination of aldehydic lipid peroxidation products. , 1989, Free radical biology & medicine.

[34]  M. Albert-Puleo Fennel and anise as estrogenic agents. , 1980, Journal of ethnopharmacology.

[35]  F. Vane,et al.  Influence of dietary vitamin E on prostaglandin biosynthesis in rat blood. , 1975, Prostaglandins.