SCENITH: A Flow Cytometry-Based Method to Functionally Profile Energy Metabolism with Single-Cell Resolution.

[1]  Maxim N. Artyomov,et al.  Immunometabolism in the Single-Cell Era. , 2020, Cell metabolism.

[2]  Sean C. Bendall,et al.  Single-cell metabolic profiling of human cytotoxic T cells , 2020, Nature biotechnology.

[3]  B. Au,et al.  Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations , 2020, Communications Biology.

[4]  Ranen Aviner The science of puromycin: From studies of ribosome function to applications in biotechnology , 2020, Computational and structural biotechnology journal.

[5]  B. Au,et al.  A novel strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations , 2020, bioRxiv.

[6]  Kamir J. Hiam,et al.  Single-cell metabolic analysis by mass cytometry reveals distinct transitional states of CD8 T cell differentiation , 2020, The Journal of Immunology.

[7]  Kamir J. Hiam,et al.  Single-cell metabolic dynamics of early activated CD8 T cells during the primary immune response to infection , 2020, bioRxiv.

[8]  Sean C. Bendall,et al.  Multiplexed Single-cell Metabolic Profiles Organize the Spectrum of Cytotoxic Human T Cells , 2020, bioRxiv.

[9]  L. Galluzzi,et al.  Macrophages and Metabolism in the Tumor Microenvironment. , 2019, Cell metabolism.

[10]  Paul J. Hoffman,et al.  Comprehensive Integration of Single-Cell Data , 2018, Cell.

[11]  E. Pearce,et al.  Metabolic interventions in the immune response to cancer , 2019, Nature Reviews Immunology.

[12]  D. Sancho,et al.  Metabolic Control of Dendritic Cell Functions: Digesting Information , 2019, Front. Immunol..

[13]  B. VanderVen,et al.  Immunometabolism at the interface between macrophages and pathogens , 2019, Nature Reviews Immunology.

[14]  R. Signer,et al.  Cell-type-specific quantification of protein synthesis in vivo , 2019, Nature Protocols.

[15]  E. Schuman,et al.  Spatially Stable Mitochondrial Compartments Fuel Local Translation during Plasticity , 2019, Cell.

[16]  D. Planchard,et al.  Overall Survival with Durvalumab after Chemoradiotherapy in Stage III NSCLC , 2018, The New England journal of medicine.

[17]  S. A. van de Pavert,et al.  SunRiSE – measuring translation elongation at single-cell resolution by means of flow cytometry , 2018, Journal of Cell Science.

[18]  Richard A. Muscat,et al.  Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding , 2018, Science.

[19]  G. Patti,et al.  Sorting cells alters their redox state and cellular metabolome , 2018, Redox biology.

[20]  I. Topisirovic,et al.  Cross-talk between protein synthesis, energy metabolism and autophagy in cancer. , 2018, Current opinion in genetics & development.

[21]  M. Bergmann,et al.  Exploring Metabolic Configurations of Single Cells within Complex Tissue Microenvironments. , 2017, Cell metabolism.

[22]  S. Biswas,et al.  Metabolic regulation of macrophage phenotype and function , 2017, Immunological reviews.

[23]  D. Schadendorf,et al.  Overall Survival with Combined Nivolumab and Ipilimumab in Advanced Melanoma , 2017, The New England journal of medicine.

[24]  Shuqiang Li,et al.  A practical solution for preserving single cells for RNA sequencing , 2017, Scientific Reports.

[25]  N. Hacohen,et al.  Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors , 2017, Science.

[26]  Michael Becker,et al.  FDR-controlled metabolite annotation for high-resolution imaging mass spectrometry , 2016, Nature Methods.

[27]  J. Yewdell,et al.  Protein Translation Activity: A New Measure of Host Immune Cell Activation , 2016, The Journal of Immunology.

[28]  Monika S. Kowalczyk,et al.  Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells , 2015, Genome research.

[29]  R. Schreiber,et al.  Metabolic Competition in the Tumor Microenvironment Is a Driver of Cancer Progression , 2015, Cell.

[30]  M. Buck,et al.  T cell metabolism drives immunity , 2015, The Journal of experimental medicine.

[31]  Evan Z. Macosko,et al.  Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets , 2015, Cell.

[32]  A. Regev,et al.  Spatial reconstruction of single-cell gene expression , 2015, Nature Biotechnology.

[33]  B. Faubert,et al.  CD8 memory T cells have a bioenergetic advantage that underlies their rapid recall ability , 2013, Proceedings of the National Academy of Sciences.

[34]  Irah L. King,et al.  Inhibition of Mechanistic Target of Rapamycin Promotes Dendritic Cell Activation and Enhances Therapeutic Autologous Vaccination in Mice , 2012, The Journal of Immunology.

[35]  K. Yarasheski,et al.  Commitment to glycolysis sustains survival of NO-producing inflammatory dendritic cells. , 2012, Blood.

[36]  M. Teitell,et al.  Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells , 2012, Nature Protocols.

[37]  G. V. D. van der Windt,et al.  Mitochondrial respiratory capacity is a critical regulator of CD8+ T cell memory development. , 2012, Immunity.

[38]  T. Holowka,et al.  Toll-like receptor-induced changes in glycolytic metabolism regulate dendritic cell activation. , 2010, Blood.

[39]  Hadley Wickham,et al.  ggplot2 - Elegant Graphics for Data Analysis (2nd Edition) , 2017 .

[40]  Russell G. Jones,et al.  Enhancing CD8 T-cell memory by modulating fatty acid metabolism , 2009, Nature.

[41]  Philippe Pierre,et al.  SUnSET, a nonradioactive method to monitor protein synthesis , 2009, Nature Methods.

[42]  J. Rathmell,et al.  Cytokine stimulation promotes glucose uptake via phosphatidylinositol-3 kinase/Akt regulation of Glut1 activity and trafficking. , 2007, Molecular biology of the cell.

[43]  Z. Trajanoski,et al.  Type, Density, and Location of Immune Cells Within Human Colorectal Tumors Predict Clinical Outcome , 2006, Science.

[44]  H. Yanagawa,et al.  Specific bonding of puromycin to full-length protein at the C-terminus. , 2000, Nucleic acids research.

[45]  E. Miyamoto-Sato,et al.  Fluorescence labeling of the C‐terminus of proteins with a puromycin analogue in cell‐free translation systems , 1999, FEBS letters.

[46]  F. Buttgereit,et al.  A hierarchy of ATP-consuming processes in mammalian cells. , 1995, The Biochemical journal.

[47]  P. Schimmel GTP hydrolysis in protein synthesis: two for Tu? , 1993, Science.

[48]  D. Roos,et al.  Changes in the carbohydrate metabolism of mitogenically stimulated human peripheral lymphocytes. II. Relative importance of glycolysis and oxidative phosphorylation on phytohaemagglutinin stimulation. , 1973, Experimental cell research.

[49]  J. Tata,et al.  Protein synthesis by membrane-bound and free ribosomes of secretory and non-secretory tissues. , 1971, The Biochemical journal.

[50]  K Kurihara,et al.  Determination of the number of active muscle ribosomes: effect of diabetes and insulin. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[51]  O. Warburg,et al.  Stoffwechsel der weißen Blutzellen , 1958 .

[52]  Thomas R. Gingeras,et al.  STAR: ultrafast universal RNA-seq aligner , 2013, Bioinform..

[53]  Cedric E. Ginestet ggplot2: Elegant Graphics for Data Analysis , 2011 .