Laboratory-based x-ray micro-tomography with submicron resolution

X-ray Microtomography bridges the 3D analysis gap between conventional x-ray tomography and TEM tomography. The use of a laboratory-based microfocus source opens up the opportunity to gain additional benefits from in-line phase contrast for enhancing the visibility of fine features, cracks, voids and boundaries in individual views. Coupled with phase retrieval methods, such images can be used as input to conventional reconstruction algorithms for three dimensional visualization. Working at high resolution brings challenges of physical stability of the system. Software approaches to overcoming these difficulties have enabled submicron resolution 3D reconstructions.

[1]  Sasov,et al.  Desktop X‐ray microscopy and microtomography , 1998, Journal of microscopy.

[2]  Chris Jacobsen,et al.  X-ray holographic microscopy using photoresists , 1990, Annual Meeting Optical Society of America.

[3]  T E Gureyev,et al.  Quantitative X-ray projection microscopy: phase-contrast and multi-spectral imaging. , 2002, Journal of microscopy.

[4]  H. R. F. Horn,et al.  How to obtain and use X-Ray projection microscopy in the SEM† , 1978 .

[5]  P. Kirkpatrick,et al.  Formation of optical images by X-rays. , 1948, Journal of the Optical Society of America.

[6]  S. Wilkins,et al.  X-ray phase-contrast microscopy and microtomography. , 2003, Optics express.

[7]  H Yoshimura,et al.  Application of SEM-modified X-ray microscope to entomology and histology, and effects of X-ray coherence in imaging. , 2000, Journal of electron microscopy.

[8]  S. Wilkins,et al.  Phase-contrast imaging using polychromatic hard X-rays , 1996, Nature.

[9]  M. L. Le Gros,et al.  X-ray tomography generates 3-D reconstructions of the yeast, saccharomyces cerevisiae, at 60-nm resolution. , 2003, Molecular biology of the cell.

[10]  P. Cloetens,et al.  Hard X-ray microscopy with reflecting mirrors status and perspectives of the ESRF technology , 2003 .

[11]  S. Wilkins,et al.  Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object , 2002, Journal of microscopy.

[12]  A. Engström,et al.  X-Ray Microscopy , 1961, Nature.

[13]  J. Kirz,et al.  Soft X-ray microscopes and their biological applications , 1995, Quarterly Reviews of Biophysics.

[14]  B. Lengeler,et al.  A microscope for hard x rays based on parabolic compound refractive lenses , 1999 .

[15]  A. Snigirev,et al.  On the possibilities of x-ray phase contrast microimaging by coherent high-energy synchrotron radiation , 1995 .

[16]  D. Gabor A New Microscopic Principle , 1948, Nature.