In situ simultaneous detection of hepatitis C virus RNA and hepatitis C virus‐related antigens in hepatocellular carcinoma

The overwhelming evidence that chronic infection with the hepatitis C virus (HCV) is an important cause of hepatocellular carcinoma (HCC) is based on epidemiologic, case‐control, and cohort studies as well as laboratory investigations. To address better the pathogenesis of HCV infection at the single‐cell level, the authors developed a specific reproducible method for the simultaneous detection of HCV specific sequences and antigens in liver tissue, using a combination of nonradioactive in situ hybridization and immunohistochemistry.

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